{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021390564470528.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3390/ijpb15010004"}},{"identifier":{"@type":"URI","@value":"https://www.mdpi.com/2037-0164/15/1/4/pdf"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"The tgd5 Mutation Affects Plastid Structure and Causes Giant Lipid Droplet Formation in Trichomes of Arabidopsis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Trichomes, epidermal protrusions in terrestrial plants, play diverse roles in plant defense, metabolism, and development. Arabidopsis thaliana, a model plant with single-celled and non-glandular trichomes, is a valuable system for studying cell differentiation in plants. However, organelle biology in Arabidopsis trichomes is relatively underexplored. Using light and transmission electron microscopy, we investigated the phenotypes of intracellular structures in Arabidopsis trichomes caused by tgd5 mutations, which are known to disrupt lipid transfer from the endoplasmic reticulum to plastids and have a large impact on chloroplast morphology in pavement and guard cells. Significant phenotypic changes in the plastid structure were observed in tgd5 trichome cells, including the absence of plastoglobuli, the emergence of clusters of electron-dense particles in the stroma, and the possibly cup-shaped morphology of plastids. Additionally, the tgd5 mutations triggered the formation of giant, up to 15 µm in diameter, neutral lipid-containing droplets in the trichome cells, as revealed using histochemical staining with lipophilic dyes. These lipid droplets were substantially larger and more frequent in trichome cells than in other types of cells in tgd5. These findings highlight the role of TGD5 in maintaining plastid structure and implicate the unique activity of lipid metabolism in Arabidopsis trichomes.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021390564470539","@type":"Researcher","foaf:name":[{"@value":"Kanae Matsuoka"}],"jpcoar:affiliationName":[{"@value":"Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Kioicho, Tokyo 102-8554, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390564470658","@type":"Researcher","foaf:name":[{"@value":"Hiroko Kubotera"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara 903-0213, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390564470656","@type":"Researcher","foaf:name":[{"@value":"Rina Miyazaki"}],"jpcoar:affiliationName":[{"@value":"Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Kioicho, Tokyo 102-8554, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390564470663","@type":"Researcher","foaf:name":[{"@value":"Shota Moriyama"}],"jpcoar:affiliationName":[{"@value":"Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Kioicho, Tokyo 102-8554, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390564470536","@type":"Researcher","foaf:name":[{"@value":"Makoto T. Fujiwara"}],"jpcoar:affiliationName":[{"@value":"Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Kioicho, Tokyo 102-8554, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390564470528","@type":"Researcher","foaf:name":[{"@value":"Ryuuichi D. Itoh"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara 903-0213, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20370164"}],"prism:publicationName":[{"@value":"International Journal of Plant Biology"}],"dc:publisher":[{"@value":"MDPI AG"}],"prism:publicationDate":"2024-01-09","prism:volume":"15","prism:number":"1","prism:startingPage":"46","prism:endingPage":"53"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.mdpi.com/2037-0164/15/1/4/pdf"}],"createdAt":"2024-01-09","modifiedAt":"2025-10-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=plastoglobule","dc:title":"plastoglobule"},{"@id":"https://cir.nii.ac.jp/all?q=chloroplast","dc:title":"chloroplast"},{"@id":"https://cir.nii.ac.jp/all?q=QH301-705.5","dc:title":"QH301-705.5"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=leaf%20epidermis","dc:title":"leaf epidermis"},{"@id":"https://cir.nii.ac.jp/all?q=oil%20body","dc:title":"oil body"},{"@id":"https://cir.nii.ac.jp/all?q=plastid%20ultrastructure","dc:title":"plastid ultrastructure"},{"@id":"https://cir.nii.ac.jp/all?q=Biology%20(General)","dc:title":"Biology (General)"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782018127488","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19K05831"},{"@type":"JGN","@value":"JP19K05831"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K05831/"}],"notation":[{"@language":"ja","@value":"シロイヌナズナの花粉および気孔発生時の色素体増殖・分配ダイナミクス"},{"@language":"en","@value":"Proliferation and partitioning of plastids during pollen and stomatal development in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1040858752026202624","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23K05820"},{"@type":"JGN","@value":"JP23K05820"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K05820/"}],"notation":[{"@language":"ja","@value":"色素体のストロミュール形成における小胞体とオートファジーの役割の解明"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233964796160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Eukaryotic lipid metabolic pathway is essential for functional chloroplasts and CO\n                    <sub>2</sub>\n                    and light responses in\n                    <i>Arabidopsis</i>\n                    guard cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142414307584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"TGD5 is required for normal morphogenesis of non‐mesophyll plastids, but not mesophyll chloroplasts, in <i>Arabidopsis</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393792263296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Non-vesicular glycerolipids transport in plant cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393792330112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A polarized supercell produces specialized metabolites in cannabis trichomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621567889280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Isolation and analysis of a stromule‐overproducing <i>Arabidopsis</i> mutant suggest the role of <i>PARC6</i> in plastid morphology maintenance in the leaf epidermis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302869446017280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Protective and defensive roles of non-glandular trichomes against multiple stresses: structure–function coordination"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870454499328","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Plastoglobules: A hub of lipid metabolism in the chloroplast"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870455350016","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The overlooked functions of trichomes: Water absorption and metal detoxication"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565168158743680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"PARC6, a novel chloroplast division factor, influences FtsZ assembly and is required for recruitment of PDV1 during chloroplast division in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093987281280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Trichomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360579814652964736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana"}]},{"@id":"https://cir.nii.ac.jp/crid/1360582642587759232","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular Mechanisms of Plant Trichome Development"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894515553024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"CDP1, a novel component of chloroplast division site positioning system in Arabidopsis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370240036224","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Identification of a chloroplast division mutant coding for ARC6H, an ARC6 homolog that plays a nonredundant role"}]},{"@id":"https://cir.nii.ac.jp/crid/1363946393949009920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Arabidopsis PARC6 Is Critical for Plastid Morphogenesis in Pavement, Trichome, and Guard Cells in Leaf Epidermis"}]},{"@id":"https://cir.nii.ac.jp/crid/1370021390564470657","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 Interacts with TGD1, TGD2, and TGD4 to Facilitate Lipid Transfer from the Endoplasmic Reticulum to Plastids"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.3390/ijpb15010004"},{"@type":"KAKEN","@value":"PRODUCT-24989897"},{"@type":"KAKEN","@value":"PRODUCT-24960853"},{"@type":"OPENAIRE","@value":"doi_dedup___::89e567b7474a46f8e50808255fa24b04"}]}