{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021390572133504.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/2024.05.06.592718"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/2024.05.06.592718"}}],"resourceType":"preprint","dc:title":[{"@value":"Geometrically-engineered organoid units and their assembly for pre-construction of organ structures"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                <jats:p>Regenerative medicine is moving from the nascent to the transitional stage as researchers are actively engaged in creating mini-organs from pluripotent stem cells to construct artificial models of physiological and pathological conditions. Currently, mini-organs can express higher-order functions, but their size is limited to the order of a few millimeters. Therefore, one of the ultimate goals of regenerative medicine, “organ replication and transplantation with organoid,” remains a major obstacle. 3D bioprinting technology is expected to be an innovative breakthrough in this field, but various issues have been raised, such as cell damage, versatility of bioink, and printing time. In this study, we established a method for fabricating, connecting, and assembling organoid units of various shapes independent of cell type, extracellular matrix, and adhesive composition (unit construction method). We also fabricated kidney tissue-like structures using three types of parenchymal and interstitial cells that compose the human kidney and obtained findings suggesting the possibility of crosstalk between the units. We anticipate that this technique will bring us closer to the realization of highly efficient and rapid fabrication of full-scale organoids that can withstand organ transplantation.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021390572133504","@type":"Researcher","foaf:name":[{"@value":"Ayaka Kadotani"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390572133506","@type":"Researcher","foaf:name":[{"@value":"Gen Hayase"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276167623552","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80624816"},{"@type":"NRID","@value":"1000080624816"},{"@type":"NRID","@value":"9000308712062"},{"@type":"NRID","@value":"9000403036287"},{"@type":"NRID","@value":"9000402389347"},{"@type":"NRID","@value":"9000305602629"},{"@type":"NRID","@value":"9000305602651"},{"@type":"NRID","@value":"9000305602694"},{"@type":"NRID","@value":"9000304695036"},{"@type":"NRID","@value":"9000308043703"},{"@type":"NRID","@value":"9000308712804"},{"@type":"NRID","@value":"9000304305752"},{"@type":"NRID","@value":"9000403036703"},{"@type":"NRID","@value":"9000308710696"},{"@type":"NRID","@value":"9000402389645"},{"@type":"NRID","@value":"9000389437096"},{"@type":"NRID","@value":"9000240062415"},{"@type":"NRID","@value":"9000396163495"},{"@type":"NRID","@value":"9000305617594"},{"@type":"NRID","@value":"9000304977152"},{"@type":"NRID","@value":"9000283646397"},{"@type":"NRID","@value":"9000242568201"},{"@type":"NRID","@value":"9000002058803"},{"@type":"NRID","@value":"9000006219420"},{"@type":"NRID","@value":"9000392355562"},{"@type":"NRID","@value":"9000331417139"},{"@type":"NRID","@value":"9000303644370"},{"@type":"NRID","@value":"9000308731214"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/7000000459"}],"foaf:name":[{"@value":"Daisuke Yoshino"}]}],"publication":{"dc:publisher":[{"@value":"openRxiv"}],"prism:publicationDate":"2024-05-08"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/2024.05.06.592718"}],"createdAt":"2024-05-09","modifiedAt":"2026-01-15","project":[{"@id":"https://cir.nii.ac.jp/crid/1040851707391394304","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21K19893"},{"@type":"JGN","@value":"JP21K19893"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19893/"}],"notation":[{"@language":"ja","@value":"超高速ブロックビルド法の確立による臓器オルガノイドの実スケール化への挑戦"},{"@language":"en","@value":"Challenge to real scale organ organoids by establishing ultrafast block building method"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232513745024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Higher-Order Kidney Organogenesis from Pluripotent Stem Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011142935474304","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Human organoids: model systems for human biology and medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145526601216","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017285974924800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Advances in 3D bioprinting of tissues/organs for regenerative medicine and in-vitro models"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393560263296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Tissue and Organ 3D Bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393796077952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393796089728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Flow-Induced Vascular Network Formation and Maturation in Three-Dimensional Engineered Tissue"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021393796733824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A review on cell damage, viability, and functionality during 3D bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021394472956928","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Spatially controlled construction of assembloids using bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021394473606272","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A neural network for glomerulus classification based on histological images of kidney biopsy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021394473667840","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A comprehensive review of 3D bioprinting biomaterials: Properties, strategies and wound healing application"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021396149164160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biomanufacturing human tissues via organ building blocks"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021396149806592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Changes in the expression and subcellular distribution of galectin-3 in clear cell renal cell carcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021397007997568","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619726314624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vitro generation of human pluripotent stem cell derived lung organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620741733376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Organogenesis in a dish: Modeling development and disease using organoid technologies"}]},{"@id":"https://cir.nii.ac.jp/crid/1360301164104250112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302865729980160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biological characterization of breast cancer spheroid formed by fast fabrication method"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870457771648","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870458700544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870459134208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302870942455680","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Tumor organoids: synergistic applications, current challenges, and future prospects in cancer therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574092892962688","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Fiji: an open-source platform for biological-image analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094260552576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"From Shape to Function: The Next Step in Bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095384446848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A three-dimensional human neural cell culture model of Alzheimer’s disease"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576120382882432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Modeling amyloid beta and tau pathology in human cerebral organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584343744180608","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An atlas of healthy and injured cell states and niches in the human kidney"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857672702717952","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Volumetric Bioprinting of Complex Living‐Tissue Constructs within Seconds"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865818472869504","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Engineering the multiscale complexity of vascular networks"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519589625344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"An in vivo model of human small intestine using pluripotent stem cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996166063232","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"3D Bioprinting for Organ Regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996366258944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Current advances and future perspectives in extrusion-based bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471048073344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cerebral organoids model human brain development and microcephaly"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945174638592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bioprinting scale-up tissue and organ constructs for transplantation"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421347360128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Vascularized human cortical organoids (vOrganoids) model cortical development in vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895039958144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Modelling human development and disease in pluripotent stem-cell-derived gastric organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370374561408","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Recapitulating macro-scale tissue self-organization through organoid bioprinting"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319729510912","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Xenograft and organoid model systems in cancer research"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269864550272","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233270077541248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Volumetric additive manufacturing via tomographic reconstruction"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233271039435392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1101/2024.05.06.592718"},{"@type":"KAKEN","@value":"PRODUCT-25039261"}]}