{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021390585080064.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3390/ijms241914823"}},{"identifier":{"@type":"URI","@value":"https://www.mdpi.com/1422-0067/24/19/14823/pdf"}},{"identifier":{"@type":"PMID","@value":"37834271"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Retroviral Replicating Vectors Mediated Prodrug Activator Gene Therapy in a Gastric Cancer Model"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Retroviral replicating vectors (RRVs) selectively replicate and can specifically introduce prodrug-activating genes into tumor cells, whereby subsequent prodrug administration induces the death of the infected tumor cells. We assessed the ability of two distinct RRVs generated from amphotropic murine leukemia virus (AMLV) and gibbon ape leukemia virus (GALV), which infect cells via type-III sodium-dependent phosphate transporters, PiT-2 and PiT-1, respectively, to infect human gastric cancer (GC) cells. A quantitative RT-PCR showed that all tested GC cell lines had higher expression levels of PiT-2 than PiT-1. Accordingly, AMLV, encoding a green fluorescent protein gene, infected and replicated more efficiently than GALV in most GC cell lines, whereas both RRVs had a low infection rate in human fibroblasts. RRV encoding a cytosine deaminase prodrug activator gene, which converts the prodrug 5-flucytosine (5-FC) to the active drug 5-fluorouracil, showed that AMLV promoted superior 5-FC-induced cytotoxicity compared with GALV, which correlated with the viral receptor expression level and viral spread. In MKN-74 subcutaneous xenograft models, AMLV had significant antitumor effects compared with GALV. Furthermore, in the MKN-74 recurrent tumor model in which 5-FC was discontinued, the resumption of 5-FC administration reduced the tumor volume. Thus, RRV-mediated prodrug activator gene therapy might be beneficial for treating human GC.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021390585080087","@type":"Researcher","foaf:name":[{"@value":"Hiroaki Fujino"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"},{"@value":"Departments of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Hyogo 669-1330, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390585080197","@type":"Researcher","foaf:name":[{"@value":"Emiko Sonoda-Fukuda"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390585080086","@type":"Researcher","foaf:name":[{"@value":"Lisa Isoda"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"},{"@value":"Departments of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Hyogo 669-1330, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390585080076","@type":"Researcher","foaf:name":[{"@value":"Ayane Kawabe"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"},{"@value":"Departments of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Hyogo 669-1330, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390585080085","@type":"Researcher","foaf:name":[{"@value":"Toru Takarada"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"},{"@value":"Laboratory of Functional Molecular Chemistry, Kobe Pharmaceutical University, Hyogo 658-8558, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390585080202","@type":"Researcher","foaf:name":[{"@value":"Noriyuki Kasahara"}],"jpcoar:affiliationName":[{"@value":"Departments of Neurological Surgery and Radiation Oncology, University of California, San Francisco, CA 94143, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276181883648","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10441320"},{"@type":"NRID","@value":"1000010441320"},{"@type":"NRID","@value":"9000003846225"},{"@type":"NRID","@value":"9000413512976"},{"@type":"NRID","@value":"9000252992230"},{"@type":"NRID","@value":"9000252990410"},{"@type":"NRID","@value":"9000401811884"},{"@type":"NRID","@value":"9000017383644"},{"@type":"NRID","@value":"9000392692923"},{"@type":"NRID","@value":"9000401811221"},{"@type":"NRID","@value":"9000401793132"},{"@type":"NRID","@value":"9000392693433"},{"@type":"NRID","@value":"9000392689181"},{"@type":"NRID","@value":"9000392689283"},{"@type":"NRID","@value":"9000006422323"},{"@type":"NRID","@value":"9000260377051"},{"@type":"NRID","@value":"9000252991678"},{"@type":"NRID","@value":"9000392685724"},{"@type":"NRID","@value":"9000252990586"},{"@type":"NRID","@value":"9000401813308"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0142551"}],"foaf:name":[{"@value":"Shuji Kubo"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Molecular and Genetic Therapeutics, Institute of Advanced Medical Science, Hyogo Medical University, Hyogo 663-8501, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14220067"}],"prism:publicationName":[{"@value":"International Journal of Molecular Sciences"}],"dc:publisher":[{"@value":"MDPI AG"}],"prism:publicationDate":"2023-10-02","prism:volume":"24","prism:number":"19","prism:startingPage":"14823"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.mdpi.com/1422-0067/24/19/14823/pdf"}],"createdAt":"2023-10-02","modifiedAt":"2025-10-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Stomach%20Neoplasms","dc:title":"Stomach Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Leukemia%20Virus,%20Gibbon%20Ape","dc:title":"Leukemia Virus, Gibbon Ape"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Vectors","dc:title":"Genetic Vectors"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Prodrugs","dc:title":"Prodrugs"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Therapy","dc:title":"Genetic Therapy"},{"@id":"https://cir.nii.ac.jp/all?q=Article","dc:title":"Article"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040299826878096768","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23K21450"},{"@type":"JGN","@value":"JP23K21450"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K21450/"}],"notation":[{"@language":"ja","@value":"播種性癌に対する革新的な自殺遺伝子ウイルス療法の開発"},{"@language":"en","@value":"Development of innovative suicide gene virotherapy for disseminated 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