{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021390739110528.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/jmor.21666"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmor.21666"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Lateral bone ridge expansion and internal tissue replacement for vertebral body growth in Pacific bluefin tuna <i>Thunnus orientalis</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Vertebral growth is an essential developmental process to support the expansion of the vertebrate body. In teleosts, the lateral side of the vertebral bodies develops to form different structures among species in the late stages of vertebral growth, although lateral structures are not apparent in the early stages. Lateral structures are one of the structural features that determine the diversity of teleost vertebrae. However, explanations for the formation of lateral structures are conflicting because few reports have investigated the growth of teleost vertebral bodies. To clarify the growth process, we analyzed the morphological changes in the vertebral body of Pacific bluefin tuna <jats:italic>Thunnus orientalis</jats:italic> at different developmental stages using micro‐computed tomography (CT) scans. The micro‐CT scans showed that the vertebral centrum formed a plate‐like ridge on the lateral side along the cranial–caudal direction and extended laterally with increasing thickness. Simultaneously, the proximal region of the lateral ridges became porous as the vertebrae grew to form bone marrow cavities. Furthermore, we used histological observations to describe the relationship between these morphological changes and osteoblast and osteoclast activities. Osteoblasts accumulated on the distal edges of the lateral ridges, whereas osteoclasts were distributed in the bone marrow cavities. These observations suggest that bone resorption occurs proximally to form bone marrow cavities in addition to bone synthesis at the edges of the lateral ridges. The bone marrow cavities were occupied by blood vessels, extracellular matrix, and adipocytes, and the internal tissue composition changed to increase the area of adipose tissue. Because the ratio of bone volume decreases in large vertebrae, bone formation and resorption are regulated to separate the external cortical and internal trabecular bones to support the vertebrae. This study is the first to report the formation of lateral structures and can be applied to similar lateral structures in the vertebrae of other teleost species.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021390739110656","@type":"Researcher","foaf:name":[{"@value":"Misaki Sakashita"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Biological Science Tokyo University of Science  Noda Chiba Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326216920192","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10252503"},{"@type":"NRID","@value":"1000010252503"},{"@type":"NRID","@value":"9000364895671"},{"@type":"NRID","@value":"9000018373988"},{"@type":"NRID","@value":"9000242736212"},{"@type":"NRID","@value":"9000002741438"},{"@type":"NRID","@value":"9000308381144"},{"@type":"NRID","@value":"9000257182304"},{"@type":"NRID","@value":"9000004953007"},{"@type":"NRID","@value":"9000301751261"},{"@type":"NRID","@value":"9000412684402"},{"@type":"NRID","@value":"9000002201744"},{"@type":"NRID","@value":"9000022007553"},{"@type":"NRID","@value":"9000017215817"},{"@type":"NRID","@value":"9000397806641"},{"@type":"NRID","@value":"9000309988109"},{"@type":"NRID","@value":"9000311785732"},{"@type":"NRID","@value":"9000331089969"},{"@type":"NRID","@value":"9000403030399"},{"@type":"NRID","@value":"9000241855698"},{"@type":"NRID","@value":"9000241185748"},{"@type":"NRID","@value":"9000243764349"},{"@type":"NRID","@value":"9000242921742"},{"@type":"NRID","@value":"9000318155192"},{"@type":"NRID","@value":"9000262346790"},{"@type":"NRID","@value":"9000254841047"},{"@type":"NRID","@value":"9000402620710"},{"@type":"NRID","@value":"9000241446683"},{"@type":"NRID","@value":"9000241591728"},{"@type":"NRID","@value":"9000255709165"},{"@type":"NRID","@value":"9000256051936"},{"@type":"NRID","@value":"9000397940291"},{"@type":"NRID","@value":"9000397856130"},{"@type":"NRID","@value":"9000020398045"},{"@type":"NRID","@value":"9000287361350"},{"@type":"NRID","@value":"9000002201660"},{"@type":"NRID","@value":"9000366122714"},{"@type":"NRID","@value":"9000252793688"},{"@type":"NRID","@value":"9000016770226"},{"@type":"NRID","@value":"9000392353533"},{"@type":"NRID","@value":"9000256038703"},{"@type":"NRID","@value":"9000246811365"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/shigerukondo"}],"foaf:name":[{"@value":"Shigeru Kondo"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Frontier Biosciences Osaka University  Suita Osaka Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021390739110528","@type":"Researcher","foaf:name":[{"@value":"Naoyuki Wada"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Biological Science Tokyo University of Science  Noda Chiba Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03622525"},{"@type":"EISSN","@value":"10974687"}],"prism:publicationName":[{"@value":"Journal of Morphology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2023-12-21","prism:volume":"285","prism:number":"2"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://creativecommons.org/licenses/by-nc/4.0/"],"url":[{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmor.21666"}],"createdAt":"2023-12-21","modifiedAt":"2024-03-01","project":[{"@id":"https://cir.nii.ac.jp/crid/1040291932571032704","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22K15154"},{"@type":"JGN","@value":"JP22K15154"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K15154/"}],"notation":[{"@language":"ja","@value":"外力依存的なコラーゲン線維の配列変化を介した骨の形態形成メカニズムの解明"}]},{"@id":"https://cir.nii.ac.jp/crid/1040849376022296704","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"20H05949"},{"@type":"JGN","@value":"JP20H05949"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05949/"}],"notation":[{"@language":"ja","@value":"構造最適化による形態形成原理の解明とその工学応用"},{"@language":"en","@value":"Elucidation of morphogenesis principles by structural optimization and its engineering applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1040858752026208000","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23K05833"},{"@type":"JGN","@value":"JP23K05833"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K05833/"}],"notation":[{"@language":"ja","@value":"ゼブラフィッシュ鰭条の再生極性を反映する上皮構造の形成メカニズム"},{"@language":"en","@value":"Analysis of the mechanism of the epithelial structure formation reflecting the regenerative polarity of the zebrafish fin ray"}]},{"@id":"https://cir.nii.ac.jp/crid/1041693800952427136","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"20H05943"},{"@type":"JGN","@value":"JP20H05943"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05943/"}],"notation":[{"@language":"ja","@value":"コラーゲン針状結晶の組み立てによる魚類ヒレの形成"},{"@language":"en","@value":"Formation of fish fins by assembly of collagen needle-like crystals"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050287297263824384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cyclic mechanical stretch contributes to network development of osteocyte-like cells with morphological change and autophagy promotion but without preferential cell alignment in rat"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002216198966016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Osteoclasts in bone modeling, as revealed by in vivo imaging, are essential for organogenesis in fish"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144791948672","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Features of mono‐ and multinucleated bone resorbing cells of the zebrafish <i>Danio rerio</i> and their contribution to skeletal development, remodeling, and growth"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619293406848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The amazing osteocyte"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621198998400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Review of fish swimming modes for aquatic locomotion"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294645454029696","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cortical or Trabecular Bone: What’s the Difference?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294647410677120","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Design of heterothermic muscle in fish"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302869454637440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evolution of the Marrow Adipose Tissue Microenvironment"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302869454754944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Normal trabecular vertebral bone is formed via rapid transformation of mineralized spicules: A high-resolution 3D ex-vivo murine study"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302869455350528","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transforming Growth Factor Beta 1 Augments Calvarial Defect Healing and Promotes Suture Regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302869455656704","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Interspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183670352768","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Measuring the swimming behaviour of a reared Pacific bluefin tuna in a submerged aquaculture net cage"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094046044928","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A comparative view on mechanisms and functions of skeletal remodelling in teleost fish, with special emphasis on osteoclasts and their function"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584344431953792","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Development of a straight vertebrate body axis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584346107997056","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Scale diversity in bigeye tuna (<i>Thunnus obesus</i>): Fat‐filled trabecular scales made of cellular bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584346108014592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Length estimation of Atlantic bluefin tuna (<scp><i>Thunnus thynnus</i></scp>) using vertebrae"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584346109267712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adipogenesis in fish"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584346109475072","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Programmed conversion of hypertrophic chondrocytes into osteoblasts and marrow adipocytes within zebrafish bones"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656176611584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Role of notochord cells and sclerotome-derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses"}]},{"@id":"https://cir.nii.ac.jp/crid/1360849939688122368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparative morphological examination of vertebral bodies of teleost fish using high‐resolution micro‐CT scans"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570744360832","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Completion of the Pacific bluefin tuna Thunnus orientalis (Temminck et Schlegel) life cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570750017920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Developmental morphology of the axial skeleton of the zebrafish, <i>Danio rerio</i> (Ostariophysi: Cyprinidae)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593661444608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Three-dimensional topology optimization model to simulate the external shapes of bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857595100935424","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The enigmas of bone without osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857597197457024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comprehensive histological evaluation of bone implants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861403835001728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The phylogenetic origin and evolution of acellular bone in teleost fishes: insights into osteocyte function in bone metabolism"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865818731456640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bone Formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865818731612544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The influence of freezing and freeze-drying of tissue specimens on enzyme activity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865818731713920","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Micro-anatomical structure of the first spine of the dorsal fin of Atlantic bluefin tuna, Thunnus thynnus (Osteichthyes: Scombridae)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865818732638976","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Precursor cells from Atlantic salmon (<i>Salmo salar</i>) visceral fat holds the plasticity to differentiate into the osteogenic lineage"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865820417622400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of freezing on bone histological morphology"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865820417735808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Histological discrimination of fresh and frozen/thawed fish meat: European hake (Merluccius merluccius) as a possible model for white meat fish species"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865820418452352","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Vertebral column regionalisation in Chinook salmon, <i>Oncorhynchus tshawytscha</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865820418667136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparison of chemical, microbiological and histological changes in fresh, frozen and double frozen rainbow trout (Oncorhynchus mykiss)"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043531696640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Growth requires bone resorption at particular skeletal elements in a teleost fish with acellular bone (Oreochromis niloticus, Teleostei: Cichlidae)"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044394814080","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The teleost intervertebral region acts as a growth center of the centrum: In vivo visualization of osteoblasts and their progenitors in transgenic fish"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519098693760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3K/Akt/GSK-3<i>β</i>/<i>β</i>-Catenin Signaling Pathway"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521096797824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A central role for the notochord in vertebral patterning"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521277209472","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Matrix remodeling during endochondral ossification"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469338605824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The salmon vertebral body develops through mineralization of two preformed tissues that are encompassed by two layers of bone"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943411701760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The development, function, and design of amphicoelous vertebrae in teleost fishes1"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943700832640","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Tissue Engineering Solution for Segmental Defect Regeneration in Load-Bearing Long Bones"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419894400768","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Buried alive: How osteoblasts become osteocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420057305856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Building the backbone: the development and evolution of vertebral patterning"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893643986176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Divergence in skeletal mass and bone morphology in antarctic notothenioid fishes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843209032448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Vertebral column and associated elements in dipnoans and comparison with other fishes: Development and homology"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845034902784","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845659426944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Spine Patterning Is Guided by Segmentation of the Notochord Sheath"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318722172928","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Adipose tissue stem cells meet preadipocyte commitment: going back to the future"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319547252736","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319788952192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319878130944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Notochord segmentation may lay down the pathway for the development of the vertebral bodies in the Atlantic salmon"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320224952832","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Skeletal anomalies in reared<scp>E</scp>uropean fish larvae and juveniles. Part 1: normal and anomalous skeletogenic processes"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269492649984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Structural and ultrastructural changes on muscle tissue of sea bass, Dicentrarchus labrax L., after cooking and freezing"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205416276224","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Control of cell differentiation by mechanical stress"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/jmor.21666"},{"@type":"KAKEN","@value":"PRODUCT-24960896"},{"@type":"KAKEN","@value":"PRODUCT-25336528"},{"@type":"KAKEN","@value":"PRODUCT-25166357"},{"@type":"KAKEN","@value":"PRODUCT-24803198"},{"@type":"OPENAIRE","@value":"doi_________::afcfe1bc3e08929a4b8ec89e63162158"}]}