{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021391865360512.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1186/s13007-023-01124-9"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/content/pdf/10.1186/s13007-023-01124-9.pdf"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/article/10.1186/s13007-023-01124-9/fulltext.html"}},{"identifier":{"@type":"HANDLE","@value":"11588/949307"}},{"identifier":{"@type":"HANDLE","@value":"20.500.14243/471747"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:sec>\n                <jats:title>Background</jats:title>\n                <jats:p>The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the <jats:italic>Fok</jats:italic>I nuclease (mitoTALEN) and targeted base editing by the DddA cytidine deaminase (mitoTALECD), to edit, for the first time, the mitochondrial genome of potato, a vegetatively propagated crop. The two methods were used to knock out the same mitochondrial target sequence (<jats:italic>orf125</jats:italic>).</jats:p>\n              </jats:sec><jats:sec>\n                <jats:title>Results</jats:title>\n                <jats:p>Targeted chondriome deletions of different sizes (236–1066 bp) were induced by mitoTALEN due to DSB repair through ectopic homologous recombination of short direct repeats (11–12 bp) present in the target region. Furthermore, in one case, the induced DSB and subsequent repair resulted in the amplification of an already present substoichiometric molecule showing a 4288 bp deletion spanning the target sequence. With the mitoTALECD approach, both nonsense and missense mutations could be induced by base substitution. The deletions and single nucleotide mutations were either homoplasmic or heteroplasmic. The former were stably inherited in vegetative offspring.</jats:p>\n              </jats:sec><jats:sec>\n                <jats:title>Conclusions</jats:title>\n                <jats:p>Both editing approaches allowed us to obtain plants with precisely modified mitochondrial genomes at high frequency. The use of the same plant genotype and mtDNA region allowed us to compare the two methods for efficiency, accuracy, type of modifications induced and stability after vegetative propagation.</jats:p>\n              </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021391865360513","@type":"Researcher","foaf:name":[{"@value":"Alessandro Nicolia"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360644","@type":"Researcher","foaf:name":[{"@value":"Nunzia Scotti"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360541","@type":"Researcher","foaf:name":[{"@value":"Giovanna Festa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360528","@type":"Researcher","foaf:name":[{"@value":"Gaetano Aufiero"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360526","@type":"Researcher","foaf:name":[{"@value":"Nunzio D’Agostino"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360648","@type":"Researcher","foaf:name":[{"@value":"Lorenza Sannino"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801181757952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00396938"},{"@type":"NRID","@value":"1000000396938"},{"@type":"ORCID","@value":"0000-0002-9537-1626"},{"@type":"NRID","@value":"9000001398783"},{"@type":"NRID","@value":"9000391661479"},{"@type":"NRID","@value":"9000364983223"},{"@type":"NRID","@value":"9000398788354"},{"@type":"NRID","@value":"9000002146442"},{"@type":"NRID","@value":"9000018600624"},{"@type":"NRID","@value":"9000359881255"},{"@type":"NRID","@value":"9000391935397"},{"@type":"NRID","@value":"9000412058770"},{"@type":"NRID","@value":"9000006230703"},{"@type":"NRID","@value":"9000018218780"},{"@type":"NRID","@value":"9000387431005"},{"@type":"NRID","@value":"9000375922351"},{"@type":"NRID","@value":"9000391951728"},{"@type":"NRID","@value":"9000253001371"},{"@type":"NRID","@value":"9000391929977"},{"@type":"NRID","@value":"9000409505944"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ShinichiArimura"}],"foaf:name":[{"@value":"Shin-ichi Arimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391865360529","@type":"Researcher","foaf:name":[{"@value":"Teodoro Cardi"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"17464811"}],"prism:publicationName":[{"@value":"Plant Methods"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2024-01-05","prism:volume":"20","prism:number":"1"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://link.springer.com/content/pdf/10.1186/s13007-023-01124-9.pdf"},{"@id":"https://link.springer.com/article/10.1186/s13007-023-01124-9/fulltext.html"}],"createdAt":"2024-01-05","modifiedAt":"2024-01-05","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mitochondrial%20DNA%20editing,%20MitoTALEN,%20MitoTALECD,%20Genome%20editing,%20Base%20editing,%20Chondriome,%20Double%20strand%20break,%20Homologous%20recombination,%20Solanum%20tuberosum","dc:title":"Mitochondrial DNA editing, MitoTALEN, MitoTALECD, Genome editing, Base editing, Chondriome, Double strand break, Homologous recombination, Solanum tuberosum"},{"@id":"https://cir.nii.ac.jp/all?q=QH301-705.5","dc:title":"QH301-705.5"},{"@id":"https://cir.nii.ac.jp/all?q=Research","dc:title":"Research"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20culture","dc:title":"Plant culture"},{"@id":"https://cir.nii.ac.jp/all?q=MitoTALECD","dc:title":"MitoTALECD"},{"@id":"https://cir.nii.ac.jp/all?q=Double%20strand%20break","dc:title":"Double strand break"},{"@id":"https://cir.nii.ac.jp/all?q=SB1-1110","dc:title":"SB1-1110"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20editing","dc:title":"Base editing"},{"@id":"https://cir.nii.ac.jp/all?q=Chondriome","dc:title":"Chondriome"},{"@id":"https://cir.nii.ac.jp/all?q=MitoTALEN","dc:title":"MitoTALEN"},{"@id":"https://cir.nii.ac.jp/all?q=Mitochondrial%20DNA%20editing","dc:title":"Mitochondrial DNA editing"},{"@id":"https://cir.nii.ac.jp/all?q=Homologous%20recombination","dc:title":"Homologous recombination"},{"@id":"https://cir.nii.ac.jp/all?q=Biology%20(General)","dc:title":"Biology (General)"},{"@id":"https://cir.nii.ac.jp/all?q=Solanum%20tuberosum","dc:title":"Solanum tuberosum"},{"@id":"https://cir.nii.ac.jp/all?q=Genome%20editing","dc:title":"Genome editing"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040566775637627392","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"19KK0391"},{"@type":"JGN","@value":"JP19KK0391"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19KK0391/"}],"notation":[{"@language":"ja","@value":"mitoTALEN法を用いた植物ミトコンドリアゲノム修復と細胞質雄性不稔の解析"},{"@language":"en","@value":"Plant mitochondrial genome repair and cytoplasmic male sterility using the mitoTALEN 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