{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021391872688512.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41429-023-00679-y"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41429-023-00679-y.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41429-023-00679-y"}},{"identifier":{"@type":"PMID","@value":"38001285"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Two new adenopeptins B and C inhibit sphere formation of pancreatic cancer cells"}],"description":[{"notation":[{"@value":"Cancer remains one of the leading causes of death worldwide, particularly pancreatic cancer being lethal because of its aggressiveness and lack of early detection methods. A factor that contributes to malignancy are cancer stem cell-like characteristics promoted by the tumor-stromal interaction. Given that fibroblast conditioned medium (CM) promotes sphere formation of cancer cells, a cancer stem cell-like characteristic, its inhibitor could be a new anticancer agent. By exploring microbial cultures as a source, we found new compounds, namely, adenopeptins B (1) and C (2), from Acremonium sp. ESF00140. 1 and 2 selectively and potently inhibited the sphere formation of pancreatic cancer cells cultured in the fibroblast CM compared with the control medium. Oxygen consumption rate (OCR) assays showed that 1 and 2 inhibit OCR in pancreatic cancer cells. Studies of similar compounds suggested mitochondrial complex V inhibition. Therefore, results of measuring the activity of human mitochondrial complex V revealed that 1 and 2 inhibited its activity. Oligomycin A, an inhibitor of mitochondrial complex V, as well as 1 and 2, strongly inhibited the sphere formation of pancreatic cancer cells cultured in fibroblast CM. The addition of 1 and 2 to pancreatic cancer cells cultured in fibroblast CM increased reactive oxygen species (ROS) production compared with that in the control medium. In pancreatic cancer cells cultured in fibroblast CM, mitochondria significantly influence sphere formation, and targeting their function with 1 and 2 might provide a new therapeutic approach for pancreatic cancer."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021391872688399","@type":"Researcher","foaf:name":[{"@value":"Daisuke Tatsuda"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688388","@type":"Researcher","foaf:name":[{"@value":"Masahide Amemiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688403","@type":"Researcher","foaf:name":[{"@value":"Chisato Nosaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688517","@type":"Researcher","foaf:name":[{"@value":"Ryuichi Sawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688392","@type":"Researcher","foaf:name":[{"@value":"Hideyuki Muramatsu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688519","@type":"Researcher","foaf:name":[{"@value":"Masayuki Igarashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688394","@type":"Researcher","foaf:name":[{"@value":"Junjiro Yoshida"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276175171072","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50442546"},{"@type":"NRID","@value":"1000050442546"},{"@type":"CINII_AUTHOR_ID","@value":"DA19602268"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DA19602268#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDA19602268"},{"@type":"NRID","@value":"9000400189063"},{"@type":"NRID","@value":"9000010419840"},{"@type":"NRID","@value":"9000383074269"},{"@type":"NRID","@value":"9000408482421"},{"@type":"NRID","@value":"9000343331435"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/tomokazu.ohishi"}],"foaf:name":[{"@value":"Tomokazu Ohishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391872688385","@type":"Researcher","foaf:name":[{"@value":"Manabu Kawada"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00218820"},{"@type":"EISSN","@value":"18811469"}],"prism:publicationName":[{"@value":"The Journal of Antibiotics"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2023-11-24","prism:volume":"77","prism:number":"2","prism:startingPage":"73","prism:endingPage":"84"},"reviewed":"false","dc:rights":["https://www.springernature.com/gp/researchers/text-and-data-mining","https://www.springernature.com/gp/researchers/text-and-data-mining"],"url":[{"@id":"https://www.nature.com/articles/s41429-023-00679-y.pdf"},{"@id":"https://www.nature.com/articles/s41429-023-00679-y"}],"createdAt":"2023-11-24","modifiedAt":"2024-01-29","project":[{"@id":"https://cir.nii.ac.jp/crid/1040010457598535168","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22K06783"},{"@type":"JGN","@value":"JP22K06783"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K06783/"}],"notation":[{"@language":"ja","@value":"大腸がん肝転移を規定する間質由来因子PAI-1を標的とした治療戦略の確立"},{"@language":"en","@value":"Establishment of a therapeutic strategy targeting the stromal-derived factor PAI-1 that regulates liver metastasis of colorectal cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1040573407559904768","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22H04922"},{"@type":"JGN","@value":"JP22H04922"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H04922/"}],"notation":[{"@language":"ja","@value":"先端モデル動物支援プラットフォーム"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231989592832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Pulmonary Fibroblasts Induce Epithelial Mesenchymal Transition and Some Characteristics of Stem Cells in Non-Small Cell Lung Cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233408800128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Coccoquinones A and B, new anthraquinone derivatives produced by Staphylotrichum coccosporum PF1460"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233409039744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Acremopeptin, a new peptaibol from Acremonium sp. 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