{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021391877200512.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3390/applmicrobiol3040091"}},{"identifier":{"@type":"URI","@value":"https://www.mdpi.com/2673-8007/3/4/91/pdf"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Employing Active Learning in Medium Optimization for Selective Bacterial Growth"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Medium optimization and development for selective bacterial cultures are essential for isolating and functionalizing individual bacteria in microbial communities; nevertheless, it remains challenging due to the unknown mechanisms between bacterial growth and medium components. The present study first tried combining machine learning (ML) with active learning to fine-tune the medium components for the selective culture of two divergent bacteria, i.e., Lactobacillus plantarum and Escherichia coli. ML models considering multiple growth parameters of the two bacterial strains were constructed to predict the fine-tuned medium combinations for higher specificity of bacterial growth. The growth parameters were designed as the exponential growth rate (r) and maximal growth yield (K), which were calculated according to the growth curves. The eleven chemical components in the commercially available medium MRS were subjected to medium optimization and specialization. High-throughput growth assays of both strains grown separately were performed to obtain thousands of growth curves in more than one hundred medium combinations, and the resultant datasets linking the growth parameters to the medium combinations were used for the ML training. Repeated rounds of active learning (i.e., ML model construction, medium prediction, and experimental verification) successfully improved the specific growth of a single strain out of the two. Both r and K showed maximized differentiation between the two strains. A further analysis of all the data accumulated in active learning identified the decision-making medium components for growth specificity and the differentiated, determinative manner of growth decisions of the two strains. In summary, this study demonstrated the efficiency and practicality of active learning in medium optimization for selective cultures and offered novel insights into the contribution of the chemical components to specific bacterial growth.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021391877200663","@type":"Researcher","foaf:name":[{"@value":"Shuyang Zhang"}],"jpcoar:affiliationName":[{"@value":"School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Ibaraki, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021391877200531","@type":"Researcher","foaf:name":[{"@value":"Honoka Aida"}],"jpcoar:affiliationName":[{"@value":"School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Ibaraki, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326226200832","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90422401"},{"@type":"NRID","@value":"1000090422401"},{"@type":"ORCID","@value":"0000-0003-2517-5686"},{"@type":"NRID","@value":"9000413522091"},{"@type":"NRID","@value":"9000015304547"},{"@type":"NRID","@value":"9000414092147"},{"@type":"NRID","@value":"9000413486586"},{"@type":"NRID","@value":"9000413499411"},{"@type":"NRID","@value":"9000413522079"},{"@type":"NRID","@value":"9000413503006"},{"@type":"NRID","@value":"9000413504866"},{"@type":"NRID","@value":"9000413499408"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0123464"}],"foaf:name":[{"@value":"Bei-Wen Ying"}],"jpcoar:affiliationName":[{"@value":"School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Ibaraki, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"26738007"}],"prism:publicationName":[{"@value":"Applied Microbiology"}],"dc:publisher":[{"@value":"MDPI AG"}],"prism:publicationDate":"2023-12-03","prism:volume":"3","prism:number":"4","prism:startingPage":"1355","prism:endingPage":"1369"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.mdpi.com/2673-8007/3/4/91/pdf"}],"createdAt":"2023-12-04","modifiedAt":"2025-10-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=culture%20medium","dc:title":"culture medium"},{"@id":"https://cir.nii.ac.jp/all?q=machine%20learning","dc:title":"machine learning"},{"@id":"https://cir.nii.ac.jp/all?q=active%20learning","dc:title":"active learning"},{"@id":"https://cir.nii.ac.jp/all?q=medium%20optimization","dc:title":"medium optimization"},{"@id":"https://cir.nii.ac.jp/all?q=bacterial%20growth","dc:title":"bacterial growth"},{"@id":"https://cir.nii.ac.jp/all?q=Microbiology","dc:title":"Microbiology"},{"@id":"https://cir.nii.ac.jp/all?q=growth%20specificity","dc:title":"growth specificity"},{"@id":"https://cir.nii.ac.jp/all?q=QR1-502","dc:title":"QR1-502"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040851707391387136","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21K19815"},{"@type":"JGN","@value":"JP21K19815"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19815/"}],"notation":[{"@language":"ja","@value":"細胞培養のデータ駆動型最適化の基盤技術の開発"},{"@language":"en","@value":"Development of fundamental technologies for data-driven optimisation of cell culture"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050016217217678336","@type":"Article","resourceType":"学術雑誌論文(journal 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