{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021392650701696.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1182/blood.v87.1.373.bloodjournal871373"}},{"identifier":{"@type":"URI","@value":"http://ashpublications.org/blood/article-pdf/87/1/373/233291/373.pdf"}}],"dc:title":[{"@value":"Differential cytokine expression in human blood monocyte subpopulations: a polymerase chain reaction analysis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The subpopulation of strongly CD14-positive (CD14++) monocytes and monocytes coexpressing the CD16 antigen and low levels of CD14 (CD14+/CD16+ cells) were isolated by fluorescence-activated cell sorting (FACS) followed by stimulation with lipopolysaccharide (LPS) at 1 micrograms/mL. Polymerase chain reaction (PCR) after reverse transcription of isolated mRNA (RT-PCR) revealed similar levels of tumor necrosis factor (TNF) transcripts in both subpopulations. By contrast, transcripts for interleukin-10 (IL-10) were only detectable in CD14++ monocytes, whereas CD14+/CD16+ cells produced no detectable IL-10 transcripts after 4 hours. Only after 16 hours of LPS stimulation was a low level of IL-10 transcripts discernible in CD14+/CD16+ monocytes. The same pattern was seen at the protein level in that TNF in LPS-stimulated supernatants was comparable for both subpopulations, whereas IL-10 was detected in CD14++ monocytes but not in CD14+/CD16+ cells. To avoid interference of cell activation by CD14 and CD16 antibodies, cells were also isolated based on the high and low level of CD33 antigen expression. Again, weakly CD33-positive cells, which comprise the CD14+/CD16+ cells, showed no or only minimal IL-10 mRNA. When comparing blood monocyte subpopulations with alveolar macrophages (AM), AM showed high levels of LPS-stimulated TNF, whereas IL-10 transcripts were undetectable. Our data show that CD14+/CD16+ blood monocytes produce high levels of proinflammatory cytokines like TNF, whereas the anti-inflammatory IL-10 is low or absent, a pattern similar to what is seen in AM.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021392650701698","@type":"Researcher","foaf:name":[{"@value":"M Frankenberger"}],"jpcoar:affiliationName":[{"@value":"Institute for Immunology, University of Munich, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1380021392650701696","@type":"Researcher","foaf:name":[{"@value":"T Sternsdorf"}],"jpcoar:affiliationName":[{"@value":"Institute for Immunology, University of Munich, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1380021392650701699","@type":"Researcher","foaf:name":[{"@value":"H Pechumer"}],"jpcoar:affiliationName":[{"@value":"Institute for Immunology, University of Munich, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1380021392650701697","@type":"Researcher","foaf:name":[{"@value":"A Pforte"}],"jpcoar:affiliationName":[{"@value":"Institute for Immunology, University of Munich, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1380021392650701700","@type":"Researcher","foaf:name":[{"@value":"HW Ziegler-Heitbrock"}],"jpcoar:affiliationName":[{"@value":"Institute for Immunology, University of Munich, Germany."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00064971"},{"@type":"EISSN","@value":"15280020"}],"prism:publicationName":[{"@value":"Blood"}],"dc:publisher":[{"@value":"American Society of Hematology"}],"prism:publicationDate":"1996-01-01","prism:volume":"87","prism:number":"1","prism:startingPage":"373","prism:endingPage":"377"},"reviewed":"false","url":[{"@id":"http://ashpublications.org/blood/article-pdf/87/1/373/233291/373.pdf"}],"createdAt":"2019-10-13","modifiedAt":"2019-11-20","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360302865745555328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Origin of Osteoclasts: Osteoclast Precursor Cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1182/blood.v87.1.373.bloodjournal871373"},{"@type":"CROSSREF","@value":"10.11005/jbm.2023.30.2.127_references_DOI_VqkPwtBkO0QrXe0NvTuh3QUe03X"}]}