{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360021393790068992.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/bit.26473"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.26473"}},{"identifier":{"@type":"URI","@value":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/bit.26473"}}],"dc:title":[{"@value":"A synthetic biology approach to transform <i>Yarrowia lipolytica</i> into a competitive biotechnological producer of β‐carotene"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:sec><jats:label/><jats:p>The increasing market demands of β‐carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. <jats:italic>Yarrowia lipolytica</jats:italic>, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl‐CoA. We first found that a lipid overproducer strain was capable of producing more β‐carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter‐gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. β‐carotene production was further increased in controlled conditions using a fed‐batch fermentation. A total production of β‐carotene of 6.5 g/L and 90 mg/g DCW with a concomitant production of 42.6 g/L of lipids was achieved. Such high titers suggest that engineered <jats:italic>Y. lipolytica</jats:italic> is a competitive producer organism of β‐carotene.</jats:p></jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380021393790068993","@type":"Researcher","foaf:name":[{"@value":"Macarena Larroude"}],"jpcoar:affiliationName":[{"@value":"BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021393790068995","@type":"Researcher","foaf:name":[{"@value":"Ewelina Celinska"}],"jpcoar:affiliationName":[{"@value":"Department of Biotechnology and Food Microbiology Poznan University of Life Sciences Poznan Poland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021393790068997","@type":"Researcher","foaf:name":[{"@value":"Alexandre Back"}],"jpcoar:affiliationName":[{"@value":"BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021393790068996","@type":"Researcher","foaf:name":[{"@value":"Stephan Thomas"}],"jpcoar:affiliationName":[{"@value":"BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021393790068992","@type":"Researcher","foaf:name":[{"@value":"Jean‐Marc Nicaud"}],"jpcoar:affiliationName":[{"@value":"BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France"}]},{"@id":"https://cir.nii.ac.jp/crid/1380021393790068994","@type":"Researcher","foaf:name":[{"@value":"Rodrigo Ledesma‐Amaro"}],"jpcoar:affiliationName":[{"@value":"BIMLip, Biologie Intégrative du Métabolisme Lipidique Team, Micalis Institute, INRA, AgroParisTech Université Paris‐Saclay Jouy‐en‐Josas France"},{"@value":"Department of Bioengineering Imperial College London London UK"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00063592"},{"@type":"EISSN","@value":"10970290"}],"prism:publicationName":[{"@value":"Biotechnology and Bioengineering"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2017-11-03","prism:volume":"115","prism:number":"2","prism:startingPage":"464","prism:endingPage":"472"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.26473"},{"@id":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/bit.26473"}],"createdAt":"2017-10-07","modifiedAt":"2025-10-08","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050016217217678336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Machine learning-assisted medium optimization revealed the discriminated strategies for improved production of the foreign and native metabolites"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/bit.26473"},{"@type":"CROSSREF","@value":"10.1016/j.csbj.2023.04.020_references_DOI_1sjEwnQ4U3xIrx968uv4j0dZx2H"},{"@type":"CROSSREF","@value":"10.1101/2023.02.20.529197_references_DOI_1sjEwnQ4U3xIrx968uv4j0dZx2H"}]}