{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360022305564565632.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.21203/rs.3.rs-2483461/v1"}},{"identifier":{"@type":"URI","@value":"https://www.researchsquare.com/article/rs-2483461/v1"}},{"identifier":{"@type":"URI","@value":"https://www.researchsquare.com/article/rs-2483461/v1.html"}}],"resourceType":"preprint","dc:title":[{"@value":"Efficient delivery of mesenchymal stem/stromal cells to injured liver by surface PEGylation"}],"description":[{"type":"abstract","notation":[{"@value":"<title>Abstract</title>\n        <p><bold>Background</bold>\nMesenchymal stem/stromal cells (MSCs) have been used in clinical trials for various diseases. These have certain notable functions such as homing to inflammation sites, tissue repair, and immune regulation. In many pre-clinical studies, MSCs administered into peripheral veins demonstrated effective therapeutic outcomes. However, most of the intravenously administered MSCs were entrapped in the lung, and homing to target sites was less than 1%. This occurred mainly because of the adhesion of MSCs to vascular endothelial cells in the lung. To prevent this adhesion, we modified the surface of MSCs with polyethylene glycol (PEG; a biocompatible polymer) using the avidin-biotin complex (ABC) method.\n<bold>Methods</bold>\nThe surface of MSCs was modified with PEG using the ABC method. Then, the cell adhesion to mouse aortic endothelial cells and the tissue distribution of PEG-modified MSCs were evaluated. Moreover, the homing to the injured liver and therapeutic effect of PEG-modified MSCs were evaluated using carbon tetrachloride-induced acute liver failure model mice.\n<bold>Results</bold>\nThe PEG modification significantly suppressed the adhesion of MSCs to cultured mouse aortic endothelial cells as well as the entrapment of MSCs in the lungs after intravenous injection in mice. PEG-modified MSCs efficiently homed to the injured liver of carbon tetrachloride-induced acute liver failure model mice. More importantly, the cells significantly suppressed serum transaminase levels and leukocyte infiltration into the injured liver.\n<bold>Conclusion</bold>\nThese results indicate that PEG modification to the surface of MSCs can suppress the lung entrapment of intravenously administered MSCs and improve their homing to the injured liver.</p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380022305564565635","@type":"Researcher","foaf:name":[{"@value":"Yukiya Takayama"}],"jpcoar:affiliationName":[{"@value":"Tokyo University of Science: Tokyo Rika Daigaku"}]},{"@id":"https://cir.nii.ac.jp/crid/1380022305564565521","@type":"Researcher","foaf:name":[{"@value":"Kosuke Kusamori"}],"jpcoar:affiliationName":[{"@value":"Tokyo University of Science: Tokyo Rika Daigaku"}]},{"@id":"https://cir.nii.ac.jp/crid/1380022305564565505","@type":"Researcher","foaf:name":[{"@value":"Yuri Katsurada"}],"jpcoar:affiliationName":[{"@value":"Tokyo University of Science: Tokyo Rika Daigaku"}]},{"@id":"https://cir.nii.ac.jp/crid/1380022305564565653","@type":"Researcher","foaf:name":[{"@value":"Makiya Nishikawa"}],"jpcoar:affiliationName":[{"@value":"Tokyo University of Science: Tokyo Rika Daigaku"}]}],"publication":{"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2023-04-27"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://www.researchsquare.com/article/rs-2483461/v1"},{"@id":"https://www.researchsquare.com/article/rs-2483461/v1.html"}],"createdAt":"2023-04-27","modifiedAt":"2023-09-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215782771328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A multifunctional envelope type nano device (MEND) for gene delivery to tumours based on the EPR effect: A strategy for 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of E-Selectin Ligands by Intracellular versus Extracellular Fucosylation Differentially Affects Osteotropism of Human Mesenchymal Stem Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021389816357376","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isPreprintOf"],"jpcoar:relatedTitle":[{"@value":"Efficient delivery of mesenchymal stem/stromal cells to injured liver by surface PEGylation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021395467731072","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transcriptional Profiling of Circulating Mononuclear Cells from Patients with Chronic Obstructive Pulmonary Disease receiving Mesenchymal Stromal Cell Infusions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021395468027008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The impact of cell surface PEGylation and short-course immunotherapy on islet graft survival in an allogeneic murine 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required for their therapeutic function"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302868759236992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Down-regulation of miR-122 after transplantation of mesenchymal stem cells in acute liver failure in mice model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302868759238144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell–selectin interaction kinetics"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302868760016384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Local but not systemic administration of mesenchymal stromal cells ameliorates fibrogenesis in regenerating livers"}]},{"@id":"https://cir.nii.ac.jp/crid/1360302868760375168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mesenchymal Stem Cells Exhibit Firm 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