{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360025431131215744.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.fochms.2024.100205"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S2666566224000121?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S2666566224000121?httpAccept=text/plain"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)"}],"description":[{"notation":[{"@value":"Common buckwheat (Fagopyrum esculentum Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The GlbNC allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in GlbNC homozygotes. Meanwhile, the GlbNB2 has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in GlbNB2 homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of GlbNB2 was more rigid than that of GlbNC. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380025431131215618","@type":"Researcher","foaf:name":[{"@value":"Takeyuki Okada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380025431131215744","@type":"Researcher","foaf:name":[{"@value":"Kohtaro Kimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380025431131215628","@type":"Researcher","foaf:name":[{"@value":"Naruha Goto"}]},{"@id":"https://cir.nii.ac.jp/crid/1380025431131215754","@type":"Researcher","foaf:name":[{"@value":"Tomoyuki Katsube-Tanaka"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"26665662"}],"prism:publicationName":[{"@value":"Food Chemistry: Molecular Sciences"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2024-07","prism:volume":"8","prism:startingPage":"100205"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license","http://creativecommons.org/licenses/by-nc-nd/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S2666566224000121?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S2666566224000121?httpAccept=text/plain"}],"createdAt":"2024-04-22","modifiedAt":"2024-06-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Common%20buckwheat","dc:title":"Common buckwheat"},{"@id":"https://cir.nii.ac.jp/all?q=Nutrition.%20Foods%20and%20food%20supply","dc:title":"Nutrition. Foods and food supply"},{"@id":"https://cir.nii.ac.jp/all?q=Allergen","dc:title":"Allergen"},{"@id":"https://cir.nii.ac.jp/all?q=GlbNB2","dc:title":"GlbNB2"},{"@id":"https://cir.nii.ac.jp/all?q=Zero-repeat%20subunit","dc:title":"Zero-repeat subunit"},{"@id":"https://cir.nii.ac.jp/all?q=TX341-641","dc:title":"TX341-641"},{"@id":"https://cir.nii.ac.jp/all?q=13S%20globulin","dc:title":"13S globulin"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040566775672508672","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"20H02967"},{"@type":"JGN","@value":"JP20H02967"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H02967/"}],"notation":[{"@language":"ja","@value":"プラスチド型熱ストレスタンパク質に着目したイネ白未熟粒の発生機構と適応技術"},{"@language":"en","@value":"Mechanism of white immature grain occurrence in rice and adaptation techniques focusing on plastid-type heat stress 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albumin g13 polypeptide, less related to Fag e 2, can be eliminated in common buckwheat (Fagopyrum esculentum Moench) seeds"}]},{"@id":"https://cir.nii.ac.jp/crid/1370025431131215749","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Development of novel common buckwheat (Fagopyrum esculentum M.) plants with lowered contents of tandem repeat-less 13S globulin-Discrimination methods of the tandem repeat-less genes"}]},{"@id":"https://cir.nii.ac.jp/crid/1370025431131215753","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Characterization of 2S albumin allergenic proteins for anaphylaxis in common buckwheat"}]},{"@id":"https://cir.nii.ac.jp/crid/1370025431131215755","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Insertion of ten amino acids into 13S globulin zero-repeat subunit improves trypsin digestibility in common buckwheat (Fagopyrum esculentum Moench) 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M.) seeds"},{"@value":"Structure and diversity of 13S globulin zero-repeat subunit, the trypsin-resistant storage protein of common buckwheat (Fagopyrum esculentum M.) seeds"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.fochms.2024.100205"},{"@type":"KAKEN","@value":"PRODUCT-25359932"},{"@type":"OPENAIRE","@value":"doi_dedup___::737598659d11dc94b47a17c7e310c134"}]}