{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360283691007486464.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.jnutbio.2015.08.009"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S095528631500203X?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S095528631500203X?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"26372092"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Methionine-dependent histone methylation at developmentally important gene loci in mouse preimplantation embryos"}],"description":[{"notation":[{"@value":"The involvement of specific nutrients in epigenetic gene regulation is a possible mechanism underlying nutrition-directed phenotypic alteration. However, the involvement of nutrients in gene-specific epigenetic regulation remains poorly understood. Methionine has been received attention as a possible nutrient involved in epigenetic modifications, as it is a precursor of the universal methyl donor for epigenetic methylation of DNA and histones. In the present study, the disruption of methionine metabolism by ethionine, an antimetabolite of methionine, induced abnormally higher expression of genes related to cell lineage differentiation and resulted in impaired blastocyst development of mouse preimplantation embryos in vitro. These effects were mitigated by the presence of methionine. Importantly, ethionine treatment induced lower trimethylation of histone H3 lysine 9 but did not affect methylation of DNA in the promoter regions of the examined genes. These results demonstrated that intact methionine metabolism is required for proper epigenetic histone modifications and normal expression of developmentally important genes during preimplantation development."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380284919180966786","@type":"Researcher","foaf:name":[{"@value":"Mari Kudo"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276164049920","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50447893"},{"@type":"NRID","@value":"1000050447893"},{"@type":"NRID","@value":"9000297476830"},{"@type":"NRID","@value":"9000414528549"},{"@type":"NRID","@value":"9000347225918"},{"@type":"NRID","@value":"9000017481181"},{"@type":"NRID","@value":"9000410476102"},{"@type":"NRID","@value":"9000331874115"},{"@type":"NRID","@value":"9000410295561"},{"@type":"NRID","@value":"9000410476781"},{"@type":"NRID","@value":"9000018562932"},{"@type":"NRID","@value":"9000389945806"},{"@type":"NRID","@value":"9000387911581"},{"@type":"NRID","@value":"9000020222585"},{"@type":"NRID","@value":"9000404512197"},{"@type":"NRID","@value":"9000399557642"},{"@type":"NRID","@value":"9000002067586"},{"@type":"NRID","@value":"9000384266289"},{"@type":"NRID","@value":"9000347225921"},{"@type":"NRID","@value":"9000404511342"},{"@type":"NRID","@value":"9000018475648"},{"@type":"NRID","@value":"9000410476646"},{"@type":"NRID","@value":"9000410293671"},{"@type":"NRID","@value":"9000410476664"},{"@type":"NRID","@value":"9000412525441"},{"@type":"NRID","@value":"9000311069813"},{"@type":"NRID","@value":"9000316202620"},{"@type":"NRID","@value":"9000410476629"},{"@type":"NRID","@value":"9000275831991"},{"@type":"NRID","@value":"9000287286209"},{"@type":"NRID","@value":"9000410476640"},{"@type":"NRID","@value":"9000399558602"},{"@type":"NRID","@value":"9000252741347"},{"@type":"NRID","@value":"9000255960879"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ikedash"}],"foaf:name":[{"@value":"Shuntaro Ikeda"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751148128000","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"20243074"},{"@type":"NRID","@value":"1000020243074"},{"@type":"CINII_AUTHOR_ID","@value":"DA16948754"},{"@type":"URI","@value":"https://ci.nii.ac.jp/author/DA16948754#entity"},{"@type":"URI","@value":"https://viaf.org/viaf/NII%7CDA16948754"},{"@type":"NRID","@value":"9000001361498"},{"@type":"NRID","@value":"9000000400379"},{"@type":"NRID","@value":"9000297476829"},{"@type":"NRID","@value":"9000347225919"},{"@type":"NRID","@value":"9000331874114"},{"@type":"NRID","@value":"9000410151451"},{"@type":"NRID","@value":"9000002123367"},{"@type":"NRID","@value":"9000021066433"},{"@type":"NRID","@value":"9000257774861"},{"@type":"NRID","@value":"9000387911582"},{"@type":"NRID","@value":"9000020222594"},{"@type":"NRID","@value":"9000404512198"},{"@type":"NRID","@value":"9000257774647"},{"@type":"NRID","@value":"9000020217939"},{"@type":"NRID","@value":"9000347225923"},{"@type":"NRID","@value":"9000410476647"},{"@type":"NRID","@value":"9000023369979"},{"@type":"NRID","@value":"9000021091805"},{"@type":"NRID","@value":"9000311069819"},{"@type":"NRID","@value":"9000275831990"},{"@type":"NRID","@value":"9000001489709"},{"@type":"NRID","@value":"9000010100510"},{"@type":"NRID","@value":"9000287286208"},{"@type":"NRID","@value":"9000021092404"},{"@type":"NRID","@value":"9000021052566"},{"@type":"NRID","@value":"9000399558601"},{"@type":"NRID","@value":"9000252741346"},{"@type":"NRID","@value":"9000255960880"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0013131"}],"foaf:name":[{"@value":"Miki Sugimoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1380284919180966784","@type":"Researcher","foaf:name":[{"@value":"Shinichi Kume"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09552863"}],"prism:publicationName":[{"@value":"The Journal of Nutritional Biochemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2015-12","prism:volume":"26","prism:number":"12","prism:startingPage":"1664","prism:endingPage":"1669"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S095528631500203X?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S095528631500203X?httpAccept=text/plain"}],"createdAt":"2015-08-20","modifiedAt":"2025-10-16","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20ICR","dc:title":"Mice, Inbred ICR"},{"@id":"https://cir.nii.ac.jp/all?q=Lysine","dc:title":"Lysine"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Developmental","dc:title":"Gene Expression Regulation, Developmental"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Differentiation","dc:title":"Cell Differentiation"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Methylation","dc:title":"DNA Methylation"},{"@id":"https://cir.nii.ac.jp/all?q=Chromatin","dc:title":"Chromatin"},{"@id":"https://cir.nii.ac.jp/all?q=Epigenesis,%20Genetic","dc:title":"Epigenesis, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Histones","dc:title":"Histones"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Blastocyst","dc:title":"Blastocyst"},{"@id":"https://cir.nii.ac.jp/all?q=Methionine","dc:title":"Methionine"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Lineage","dc:title":"Cell Lineage"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256825016192","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K07779"},{"@type":"JGN","@value":"JP15K07779"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07779/"}],"notation":[{"@language":"ja","@value":"哺乳動物の初期胚におけるニュートリエピジェネティクス"},{"@language":"en","@value":"Nutriepigenetics in mammalian preimplantation embryos"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050288525434415744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle"}]},{"@id":"https://cir.nii.ac.jp/crid/1050564285810937728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143518081280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Differential apoptotic staining of mammalian blastocysts based on double immunofluorescent CDX2 and active caspase-3 staining"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144573288960","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Expression of methylation pathway enzymes in bovine oocytes and preimplantation embryos"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146278213376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos"}]},{"@id":"https://cir.nii.ac.jp/crid/1360025033963776896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619212864000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Environmental and epigenetic effects upon preimplantation embryo metabolism and development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565167098288896","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Methionine Adenosyltransferase II-dependent Histone H3K9 Methylation at the COX-2 Gene Locus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183060772864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The RPMI-1640 vitamin mixture promotes bovine blastocyst development<i>in vitro</i>and downregulates gene expression of<i>TXNIP</i>with epigenetic modification of associated histones"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095111992064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"G<scp>ENETIC</scp>E<scp>FFECTS OF</scp>M<scp>ETHYLATION</scp>D<scp>IETS</scp>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096387941120","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Lineage specification in the early mouse embryo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576149724707072","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"KMT1E Mediated H3K9 Methylation Is Required for the Maintenance of Embryonic Stem Cells by Repressing Trophectoderm Differentiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567511978112","@type":"Article","resourceType":"preprint","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Comparative analysis of histone H3K4me3 modifications between early embryos and somatic tissues in cattle"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569258001408","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Endogenous folates and single-carbon metabolism in the ovarian follicle, oocyte and pre-implantation embryo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569736264576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Both the folate cycle and betaine‐homocysteine methyltransferase contribute methyl groups for DNA methylation in mouse blastocysts"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044268466432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044785223168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518458683264","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nutritional effects on oocyte and embryo development in mammals: implications for reproductive efficiency and environmental sustainability"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518625179136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Differential Growth of the Mouse Preimplantation Embryo in Chemically Defined Media1"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520202663808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"μChIP: Chromatin Immunoprecipitation for Small Cell Numbers"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993453038720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Folate Deficiency during Early-Mid Pregnancy Affects the Skeletal Muscle Transcriptome of Piglets from a Reciprocal Cross"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995144372864","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Epigenetic characterization of the early embryo with a chromatin immunoprecipitation protocol applicable to small cell populations"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469292046848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"DNA methylation dynamics during epigenetic reprogramming in the germline and preimplantation embryos"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943561749760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Differences in early lineage segregation between mammals"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944147093120","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"DNA methylation, insulin resistance, and blood pressure in offspring determined by maternal periconceptional B vitamin and methionine status"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944793768832","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nutritional Epigenetics"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544418734312448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Histone post‐translational modifications in preimplantation mouse embryos and their role in nuclear architecture"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420527022336","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Epigenetics: A New Bridge between Nutrition and Health"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420764790784","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Sulfur-Containing Amino Acids: An Overview"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421124012544","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Use of a Mouse In Vitro Fertilization Model to Understand the Developmental Origins of Health and Disease Hypothesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421335466368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A Methyl-Deficient Diet Fed to Rat Dams during the Peri-Conception Period Programs Glucose Homeostasis in Adult Male but Not Female Offspring"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896262640256","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Imprinting on distal chromosome 7 in the placenta involves repressive histone methylation independent of DNA methylation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369610325248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"On your histone mark, SET, methylate!"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370373574144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844143517824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanisms of transcriptional repression by histone lysine methylation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845892968448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Epigenetic mechanisms for nutrition determinants of later health outcomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795554201984","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Intercellular Interactions, Position, and Polarity in Establishing Blastocyst Cell Lineages and Embryonic Axes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795802646144","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795854631808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Choline deficiency alters global histone methylation and epigenetic marking at the Rel site of the calbindin 1 gene"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795943503360","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Epigenetics and Nutritional Environmental Signals"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269007351808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development"}]},{"@id":"https://cir.nii.ac.jp/crid/1370288393850644740","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Induction of a trophoblast-like phenotype by hydralazine in the p19 embryonic carcinoma cell line"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681311704320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Roles of One-carbon Metabolism in Preimplantation Period"},{"@value":"Roles of One-carbon Metabolism in Preimplantation Period : Effects on Short-term Development and Long-term Programming"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681313173376","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Methionine Requirements for the Preimplantation Bovine Embryo"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681313793536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Importance of Methionine Metabolism in Morula-to-blastocyst Transition in Bovine Preimplantation Embryos"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.jnutbio.2015.08.009"},{"@type":"KAKEN","@value":"PRODUCT-20191252"},{"@type":"OPENAIRE","@value":"doi_dedup___::14b9e5b042ad10e851305b576831e2ef"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-04003-1_references_DOI_ZwU5aL4Y0NxKiJXbOoLQ01azLH4"},{"@type":"CROSSREF","@value":"10.1038/s41598-021-87683-0_references_DOI_ZwU5aL4Y0NxKiJXbOoLQ01azLH4"},{"@type":"CROSSREF","@value":"10.1017/s2040174417000563_references_DOI_ZwU5aL4Y0NxKiJXbOoLQ01azLH4"},{"@type":"CROSSREF","@value":"10.1101/2020.11.11.379057_references_DOI_ZwU5aL4Y0NxKiJXbOoLQ01azLH4"}]}