{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360285708114367232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/acs.langmuir.6b00741"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.6b00741"}},{"identifier":{"@type":"PMID","@value":"27232007"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Atomic Force Microscopic Analysis of the Effect of Lipid Composition on Liposome Membrane Rigidity"}],"description":[{"notation":[{"@value":"Mechanical rigidity of the liposome membrane is often defined by the membrane bending modulus and is one of the determinants of liposome stability, but the quantitative experimental data are still limited to a few kinds of liposomes. Here, we used atomic force microscopy to investigate the membrane bending moduli of liposomes by immobilizing them on bovine serum albumin-coated glass in aqueous medium. The following lipids were used for liposome preparation: egg yolk phosphatidylcholine, dioleoylphosphatidylcholine, hydrogenated soybean phosphatidylcholine, dipalmitoylphosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, cholesterol, and N-(carbonylmethoxypoly(ethylene glycol) 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine. By using liposomes of various compositions, we showed that the thermodynamic phase state of the membrane rather than the electric potential or liposome surface modification with poly(ethylene glycol) is the predominant determinant of the bending modulus, which decreased in the following order: solid orderedliquid orderedliquid disordered. By using the generalized polarization value of the Laurdan fluorescent probe, we investigated membrane rigidity in terms of membrane fluidity. Atomic force microscopic analysis was superior to the Laurdan method, especially in evaluating the membrane rigidity of liposomes containing hydrogenated soybean phosphatidylcholine and cholesterol. Positively charged liposomes with a large bending modulus were taken up by cells more efficiently than those with a small bending modulus. These findings offer a quantitative method of analyzing the membrane rigidity of nanosized liposomes with different lipid compositions and will contribute to the control of liposome stability and cellular uptake efficiency of liposomal formulations intended for clinical use."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380285708114367234","@type":"Researcher","foaf:name":[{"@value":"Yuki Takechi-Haraya"}],"jpcoar:affiliationName":[{"@value":"Japan Agency for\rMedical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan"},{"@value":"Division\rof Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380285708114367237","@type":"Researcher","foaf:name":[{"@value":"Kumiko Sakai-Kato"}],"jpcoar:affiliationName":[{"@value":"Division\rof Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380285708114367235","@type":"Researcher","foaf:name":[{"@value":"Yasuhiro Abe"}],"jpcoar:affiliationName":[{"@value":"Division\rof Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380285708114367236","@type":"Researcher","foaf:name":[{"@value":"Toru Kawanishi"}],"jpcoar:affiliationName":[{"@value":"National Institute\rof Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380285708114367233","@type":"Researcher","foaf:name":[{"@value":"Haruhiro Okuda"}],"jpcoar:affiliationName":[{"@value":"National Institute\rof Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380285708114367232","@type":"Researcher","foaf:name":[{"@value":"Yukihiro Goda"}],"jpcoar:affiliationName":[{"@value":"Division\rof Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"07437463"},{"@type":"EISSN","@value":"15205827"}],"prism:publicationName":[{"@value":"Langmuir"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2016-06-09","prism:volume":"32","prism:number":"24","prism:startingPage":"6074","prism:endingPage":"6082"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.6b00741"}],"createdAt":"2016-05-27","modifiedAt":"2023-04-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Drug%20Compounding","dc:title":"Drug Compounding"},{"@id":"https://cir.nii.ac.jp/all?q=Liposomes","dc:title":"Liposomes"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Atomic%20Force","dc:title":"Microscopy, Atomic Force"},{"@id":"https://cir.nii.ac.jp/all?q=Lipids","dc:title":"Lipids"},{"@id":"https://cir.nii.ac.jp/all?q=Polyethylene%20Glycols","dc:title":"Polyethylene Glycols"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282256825212288","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K07915"},{"@type":"JGN","@value":"JP15K07915"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07915/"}],"notation":[{"@language":"ja","@value":"リポタンパク質受容体を介したリポソームの細胞内動態の分子メカニズム"},{"@language":"en","@value":"Molecular mechanism of intracellular uptake of liposomes mediated by lipoprotein receptors"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002216676323072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Detection of Nanosized Ordered Domains in DOPC/DPPC and DOPC/Ch Binary Lipid Mixture Systems of Large Unilamellar Vesicles Using a TEMPO Quenching Method"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144929208448","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanical properties and stability measurement of cholesterol-containing liposome on mica by atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146544682880","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Electro-mechanical permeabilization of lipid vesicles. Role of membrane tension and compressibility"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146585371008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Influence of cholesterol on phospholipid bilayers phase domains as detected by Laurdan fluorescence"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168802275712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Enhancing the Endocytosis of Phosphatidylserine-Containing Liposomes through Tim4 by Modulation of Membrane Fluidity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360016864700671488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic α-helical peptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707048120448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Liposomes with temperature-responsive reversible surface properties"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618999374720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Elastic properties of lipid bilayer containing modified lipids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619595311872","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Liposome−Cell Interactions in Vitro:  Effect of Liposome Surface Charge on the Binding and Endocytosis of Conventional and Sterically Stabilized Liposomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619837993728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of chain lengths of PEO–PPO–PEO on small unilamellar liposome morphology and stability: an AFM investigation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306905638393088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Cholesterol-end-modified–PEG vesicle: DDS carrier with long-term stability to encapsulate a drug model by facile preparation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181949541632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Involvement of scavenger receptor class B type 1 and low-density lipoprotein receptor in the internalization of liposomes into HepG2 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182509697536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Determination of Nonspherical Morphology of Doxorubicin-Loaded Liposomes by Atomic Force Microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183091300224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tuning the Viscoelasticity of Peptide Vesicles by Adjusting Hydrophobic Helical Blocks Comprising Amphiphilic Polypeptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183194200832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Heterogeneous Cationic Liposomes Modified with 3β-{<i>N</i>-[(<i>N</i>′,<i>N</i>′-Dimethylamino)ethyl]carbamoyl}cholesterol Can Induce Partial Conformational Changes in Messenger RNA and Regulate Translation in an Escherichia coli Cell-Free Translation System"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574093449980032","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Formation of Supported Phospholipid Bilayers from Unilamellar Vesicles Investigated by Atomic Force Microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094985362816","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095433747840","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension"}]},{"@id":"https://cir.nii.ac.jp/crid/1360588379388274688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Amyloid beta (Aβ) fibrillation kinetics and its impact on membrane polarity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846641542094848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Control of Liposomal Penetration into Three-Dimensional Multicellular Tumor Spheroids by Modulating Liposomal Membrane Rigidity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568477241856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Structural Role of Cholesterol in Cell Membranes: From Condensed Bilayers to Lipid Rafts"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569511209216","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Atomic force microscopy of nanometric liposome adsorption and nanoscopic membrane domain formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570340372992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bending and Puncturing the Influenza Lipid Envelope"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855570974933760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"From Liposomes to Supported, Planar Bilayer Structures on Hydrophilic and Hydrophobic Surfaces: An Atomic Force Microscopy Study"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865815503719040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Preparing Size-Controlled Liposomes Modified with Polysaccharide Derivatives for pH-Responsive Drug Delivery Applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137043434306176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of Lipid Composition on the Properties of Doxorubicin-Loaded Liposomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044500447360","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanical Properties of Nanoscopic Lipid Domains"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045330075904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Endocytosis of nanomedicines"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137046030249600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Direct method to study membrane rigidity of small vesicles based on atomic force microscope force spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137046493244416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Exploiting the enhanced permeability and retention effect for tumor targeting"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519330122368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of cholesterol on structural and mechanical properties of membranes depends on lipid chain saturation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519649909888","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Lipid Lateral Diffusion in Ordered and Disordered Phases in Raft Mixtures"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520854273792","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Imaging and force measurement of LDL and HDL by AFM in air and liquid"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993483961600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of cholesterol content on the structural and dynamic membrane properties of DMPC/DSPC large unilamellar bilayers"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995539772160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cellular Uptake of Elastic Nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995629820672","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cholesterol-induced fluid-phase immiscibility in membranes."}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996334057600","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Deformation behaviour of soft particles: a review"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468420823168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Hydration of lipoplexes commonly used in gene delivery: follow-up by laurdan fluorescence changes and quantification by differential scanning calorimetry"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468673677184","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A novel method for measuring rigidity of submicron-size liposomes with atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981469114540800","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Universal Behavior of Membranes with Sterols"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470917304960","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Physical Principles of Nanoparticle Cellular Endocytosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471108243584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bending elasticities of model membranes: influences of temperature and sterol content"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471249020672","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Recent developments in the field of bending rigidity measurements on membranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943773123328","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Laurdan and Prodan as Polarity-Sensitive Fluorescent Membrane Probes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945495024000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Observation of liposomes of differing lipid composition in aqueous medium by means of atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945760017280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262946192131968","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cholesterol interactions with phospholipids in membranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419570471936","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Shape changes of giant liposomes induced by an asymmetric transmembrane distribution of phospholipids"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544421378715520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Simulations of a Membrane-Anchored Peptide: Structure, Dynamics, and Influence on Bilayer Properties"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893640713344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"How Phospholipid-Cholesterol Interactions Modulate Lipid Lateral Diffusion, as Revealed by Fluorescence Correlation Spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895794547456","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mechanical Properties of Freestanding Polyelectrolyte Capsules: a Quantitative Approach Based on Shell Theory"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369068326912","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Transbilayer Movement of Phospholipids at the Main Phase Transition of Lipid Membranes: Implications for Rapid Flip-Flop in Biological Membranes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107369505922560","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of grafted PEG on liposome size and on compressibility and packing of lipid bilayer"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370385282688","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"New Developments in Liposomal Drug Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843500931840","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of cholesterol on the rigidity of saturated and unsaturated membranes: fluctuation and electrodeformation analysis of giant vesicles"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843906955136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Probing small unilamellar EggPC vesicles on mica surface by atomic force microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844035175808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Hydration of surfactant-modified and PEGylated cationic cholesterol-based liposomes and corresponding lipoplexes by monitoring a fluorescent probe and the dielectric relaxation time"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844506347776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"New-Concept Chemotherapy by Nanoparticles of Biodegradable Polymers: Where Are We Now?"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844575163776","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Phospholipid-cationic lipid interactions: influences on membrane and vesicle properties"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845181419904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nanoindentation studies of full and empty viral capsids and the effects of capsid protein mutations on elasticity and strength"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845264834816","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Relationship between lipid peroxidation and rigidity in L-α-phosphatidylcholine-DPPC vesicles"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319838451584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Structure and fluctuations of a single floating lipid bilayer"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319860912384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Emerging Research and Clinical Development Trends of Liposome and Lipid Nanoparticle Drug Delivery Systems"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320437698944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793884785024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793970893184","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Influence of cholesterol on liposome stability and on in vitro drug release"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794060153088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Liposomal drug delivery systems: From concept to clinical applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795036801280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Comparative Properties and Methods of Preparation of Lipid Vesicles (Liposomes)"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268469768320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Elastic deformation and failure of lipid bilayer membranes containing cholesterol"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679153790464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Thermotropic Phase Behavior of Hydrogenated Soybean Phosphatidylcholine–Cholesterol Binary Liposome Membrane"}]},{"@id":"https://cir.nii.ac.jp/crid/1390848250108732032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Instrument-Dependent Factors Affecting the Precision in the Atomic Force Microscopy Stiffness Measurement of Nanoscale Liposomes"}]},{"@id":"https://cir.nii.ac.jp/crid/1390862943886555776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Atomic Force Microscopy to Measure the Mechanical Property of Nanosized Lipid Vesicles and Its Applications"},{"@language":"ja","@value":"原子間力顕微鏡法を用いたナノ脂質膜小胞の力学的特性計測法の開発とその応用"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1021/acs.langmuir.6b00741"},{"@type":"KAKEN","@value":"PRODUCT-21178541"},{"@type":"OPENAIRE","@value":"doi_dedup___::6d17606456e3a9b7c9eb0aa0ef36ff1d"},{"@type":"CROSSREF","@value":"10.1021/acs.molpharmaceut.1c00645_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1038/s41598-022-08876-9_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1016/j.colsurfb.2019.01.007_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1016/j.xphs.2017.10.009_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1248/yakushi.23-00178-2_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1093/chemle/upae166_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1007/s10863-024-10046-7_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.3390/life13112158_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1021/acs.molpharmaceut.7b00051_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1021/acs.langmuir.7b00289_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1248/cpb.c20-00067_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"},{"@type":"CROSSREF","@value":"10.1016/j.bbamem.2017.09.005_references_DOI_3qhaYxXriGrNtejBDQ6fz3tO5Xi"}]}