Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae
書誌事項
- 公開日
- 2017-11-17
- 資源種別
- journal article
- 権利情報
-
- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
-
- 10.1038/s41598-017-16163-1
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title><jats:p>Recent research in filamentous fungi has revealed that the motility of an endocytic organelle early endosome (EE) has a versatile role in many physiological functions. Here, to further examine the motility of EEs in the industrially important fungus<jats:italic>Aspergillus oryzae</jats:italic>, we visualized these organelles via the Rab5 homolog AoRab5 and identified AoHok1, a putative linker protein between an EE and a motor protein. The<jats:italic>Aohok1</jats:italic>disruptant showed retarded mycelial growth and no EE motility, in addition to an apical accumulation of EEs and peroxisomes. We further demonstrated that the<jats:italic>Aohok1</jats:italic>disruptant exhibited less sensitivity to osmotic and cell wall stresses. Analyses on the protein secretory pathway in Δ<jats:italic>Aohok1</jats:italic>cells showed that, although distribution of the endoplasmic reticulum and Golgi was not affected, formation of the apical secretory vesicle cluster Spitzenkörper was impaired, probably resulting in the observed reduction of the<jats:italic>A. oryzae</jats:italic>major secretory protein α-amylase. Moreover, we revealed that the transcript level of α-amylase-encoding gene<jats:italic>amyB</jats:italic>was significantly reduced in the<jats:italic>Aohok1</jats:italic>disruptant. Furthermore, we observed perturbed conidial and sclerotial formations, indicating a defect in cell differentiation, in the<jats:italic>Aohok1</jats:italic>disruptant. Collectively, our results suggest that EE motility is crucial for α-amylase production and cell differentiation in<jats:italic>A. oryzae</jats:italic>.</jats:p>
収録刊行物
-
- Scientific Reports
-
Scientific Reports 7 (1), 15757-, 2017-11-17
Springer Science and Business Media LLC
