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Layilin enhances the invasive ability of malignant glioma cells via SNAI1 signaling
Bibliographic Information
- Published
- 2019-09
- Resource Type
- journal article
- Rights Information
-
- https://www.elsevier.com/tdm/userlicense/1.0/
- https://www.elsevier.com/legal/tdmrep-license
- DOI
-
- 10.1016/j.brainres.2019.05.034
- Publisher
- Elsevier BV
Search this article
Description
Malignant gliomas are characterized by high invasive ability. In this study, we investigated roles of layilin, a C-type lectin-homologous protein, in the invasive ability of malignant glioma cells.Expression of layilin was investigated by western blotting in the malignant glioma cell lines of U251-MG, A172, and T98G and in astrocytes. The effects of layilin-knockdown on the expression and protein levels of snail family transcriptional repressor 1 (SNAI1), a transcriptional factor involved in the acquisition and enhancement of invasive ability in malignant gliomas, and on the expression of its target genes, matrix metalloproteinase 2 (MMP2), MMP9, and collagen type I alpha 1 chain (COL1A1), were investigated by qPCR and/or western blotting. Furthermore, the effects of layilin-knockdown on the expression and protein levels of metastasis associated 1 family member 3 (MTA3), a transcriptional repressor of SNAI1, were also investigated by qPCR and western blotting. Finally, the effects of layilin-knockdown on the invasive ability of the cells were investigated by a wound healing assay.All the tested malignant glioma cells highly expressed layilin, compared to astrocytes, one of representative glial cell types. Layilin-knockdown reduced SNAI1 both at the mRNA and protein levels in A172 cells, and consequently mRNA levels of MMP2, MMP9, and COL1A1 were also reduced. Furthermore, layilin-knockdown increased nuclear protein levels of MTA3 in A172 cells. Notably, layilin-knockdown suppressed the invasive ability of the cells.Layilin up-regulates the expression of SNAI1 via down-regulation of MTA3. This process enhances the invasive ability of malignant glioma cells.
Journal
-
- Brain Research
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Brain Research 1719 140-147, 2019-09
Elsevier BV
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Keywords
- Brain Neoplasms
- Glioma
- Collagen Type I
- Neoplasm Proteins
- Collagen Type I, alpha 1 Chain
- Gene Expression Regulation
- Matrix Metalloproteinase 9
- Astrocytes
- Cell Line, Tumor
- Humans
- Matrix Metalloproteinase 2
- Lectins, C-Type
- Neoplasm Invasiveness
- Snail Family Transcription Factors
- Signal Transduction
- Transcription Factors
Details 詳細情報について
-
- CRID
- 1360286991675871360
-
- ISSN
- 00068993
-
- PubMed
- 31145904
-
- Article Type
- journal article
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- Data Source
-
- Crossref
- KAKEN
- OpenAIRE