Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells

DOI Web Site PubMed 3 Citations 46 References Open Access
  • Naoki Yamamoto
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan
  • Noriko Hiramatsu
    Research Promotion and Support Headquarters, Fujita Health University, Aichi 470-1192, Japan
  • Mahito Ohkuma
    Department of Physiology, School of Medicine, Fujita Health University, Aichi 470-1192, Japan
  • Natsuko Hatsusaka
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan
  • Shun Takeda
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan
  • Noriaki Nagai
    Faculty of Pharmacy, Kindai University, Osaka 577-8502, Japan
  • Ei-ichi Miyachi
    Department of Physiology, School of Medicine, Fujita Health University, Aichi 470-1192, Japan
  • Masashi Kondo
    Department of Respiratory Medicine, School of Medicine, Fujita Health University, Aichi 470-1192, Japan
  • Kazuyoshi Imaizumi
    Department of Respiratory Medicine, School of Medicine, Fujita Health University, Aichi 470-1192, Japan
  • Masayuki Horiguchi
    Department of Ophthalmology, School of Medicine, Fujita Health University, Aichi 470-1192, Japan
  • Eri Kubo
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan
  • Hiroshi Sasaki
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa 920-0293, Japan

Bibliographic Information

Published
2021-03-28
Resource Type
journal article
Rights Information
  • https://creativecommons.org/licenses/by/4.0/
DOI
  • 10.3390/cells10040743
Publisher
MDPI AG

Description

<jats:p>Regenerative medicine in ophthalmology that uses induced pluripotent stem cells (iPS) cells has been described, but those studies used iPS cells derived from fibroblasts. Here, we generated iPS cells derived from iris cells that develop from the same inner layer of the optic cup as the retina, to regenerate retinal nerves. We first identified cells positive for p75NTR, a marker of retinal tissue stem and progenitor cells, in human iris tissue. We then reprogrammed the cultured p75NTR-positive iris tissue stem/progenitor (H-iris stem/progenitor) cells to create iris-derived iPS (H-iris iPS) cells for the first time. These cells were positive for iPS cell markers and showed pluripotency to differentiate into three germ layers. When H-iris iPS cells were pre-differentiated into neural stem/progenitor cells, not all cells became positive for neural stem/progenitor and nerve cell markers. When these cells were pre-differentiated into neural stem/progenitor cells, sorted with p75NTR, and used as a medium for differentiating into retinal nerve cells, the cells differentiated into Recoverin-positive cells with electrophysiological functions. In a different medium, H-iris iPS cells differentiated into retinal ganglion cell marker-positive cells with electrophysiological functions. This is the first demonstration of H-iris iPS cells differentiating into retinal neurons that function physiologically as neurons.</jats:p>

Journal

  • Cells

    Cells 10 (4), 743-, 2021-03-28

    MDPI AG

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