Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy

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  • Haolu Wang
    Therapeutics Research Centre, School of Medicine The University of Queensland, Princess Alexandra Hospital Woolloongabba QLD 4102 Australia
  • Xiaowen Liang
    Therapeutics Research Centre, School of Medicine The University of Queensland, Princess Alexandra Hospital Woolloongabba QLD 4102 Australia
  • Germain Gravot
    Department of Pharmacy University of Rennes 1 Ille‐et‐Vilaine Rennes 35043 France
  • Camilla A. Thorling
    Therapeutics Research Centre, School of Medicine The University of Queensland, Princess Alexandra Hospital Woolloongabba QLD 4102 Australia
  • Darrell H. G. Crawford
    School of Medicine The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital Greenslopes QLD 4120 Australia
  • Zhi Ping Xu
    Australian Institute for Bioengineering and Nanotechnology The University of Queensland St Lucia QLD 4072 Australia
  • Xin Liu
    Therapeutics Research Centre, School of Medicine The University of Queensland, Princess Alexandra Hospital Woolloongabba QLD 4102 Australia
  • Michael S. Roberts
    Therapeutics Research Centre, School of Medicine The University of Queensland, Princess Alexandra Hospital Woolloongabba QLD 4102 Australia

Bibliographic Information

Published
2016-06-17
Rights Information
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/jbio.201600083
Publisher
Wiley

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<jats:p>Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver <jats:italic>in vivo</jats:italic>. We anticipate that in the near future MPM‐FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real‐time histology of human liver diseases. <jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/png" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/jbio201600083-gra-0001-m.png"><jats:alt-text>magnified image</jats:alt-text></jats:graphic></jats:boxed-text></jats:p>

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