{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360292619800985600.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1444-2906.2005.01102.x"}}],"dc:title":[{"@value":"Rapid identification of eels Anguilla japonica and Anguilla anguilla by polymerase chain reaction with single nucleotide polymorphism-based specific probes"}],"description":[{"notation":[{"@value":"Standard molecular techniques, such as sequencing and restriction fragment length polymorphism analysis after polymerase chain reaction (PCR) amplification are relatively complicated, and species identification can take a long time when using such techniques. We established a quick method, using PCR with species-specific TaqMan Minor Groove Binder (MGB) probes based on single nucleotide polymorphism (SNP) to distinguish the two eel species Anguilla japonica and Anguilla anguilla. This method can be used in processed products. Partial sequences of the mitochondrial 16S rRNA gene were compared between A. japonica and A. anguilla to design a primer pair common to both A. japonica and A. anguilla and probes specific to A. japonica and A. anguilla. Different fluorescence intensities were produced in two PCR microtubes each containing A. japonica and A. anguilla-specific probes for one target sample. We observed the fluorescence intensity of PCR products in microtubes under ultraviolet transillumination, with similar results to those obtained by real-time PCR. Therefore, SNP-based PCR is a powerful tool for identifying materials of processed foods from either A. japonica or A. anguilla."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380292619800985605","@type":"Researcher","foaf:name":[{"@value":"Shiro ITOI"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292619800985603","@type":"Researcher","foaf:name":[{"@value":"Misako NAKAYA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292619800985602","@type":"Researcher","foaf:name":[{"@value":"Gen KANEKO"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292619800985604","@type":"Researcher","foaf:name":[{"@value":"Hidehiro KONDO"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292619800985600","@type":"Researcher","foaf:name":[{"@value":"Keijiro SEZAKI"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292619800985601","@type":"Researcher","foaf:name":[{"@value":"Shugo WATABE"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09199268"},{"@type":"EISSN","@value":"14442906"}],"prism:publicationName":[{"@value":"Fisheries Science"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2005-12","prism:volume":"71","prism:number":"6","prism:startingPage":"1356","prism:endingPage":"1364"},"reviewed":"false","createdAt":"2005-12-02","modifiedAt":"2019-03-20","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285711828115968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Genetic identification method for two subspecies of the Indonesian short-finned eel, Anguilla bicolor, using an allelic discrimination technique"}]},{"@id":"https://cir.nii.ac.jp/crid/1361412890311957632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Zoantharia (Cnidaria: Anthozoa: Hexacorallia) Diversity Research in Japan: Current State and Future Trends"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679434936704","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Specific Real-time PCR Detection of Monkfish Ingredients"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681395502080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"DNA analysis method for detecting the mixing of foreign species of eel among many Anguilla japonica eels"},{"@language":"ja","@value":"ＤＮＡ　鑑定による多検体ウナギ中の外国種混入判別法"},{"@language":"ja-Kana","@value":"DNA カンテイ ニ ヨル タケンタイ ウナギ チュウ ノ ガイコクシュ コンニュウ ハンベツホウ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1444-2906.2005.01102.x"},{"@type":"OPENAIRE","@value":"doi_dedup___::f1e2af3e441d0e8cada40f7fcfb4655c"},{"@type":"CROSSREF","@value":"10.2331/suisan.77.896_references_DOI_8MIx969y2M9boJkuxPfvdG7Y74T"},{"@type":"CROSSREF","@value":"10.1186/s40555-014-0057-8_references_DOI_8MIx969y2M9boJkuxPfvdG7Y74T"},{"@type":"CROSSREF","@value":"10.3136/fstr.19.759_references_DOI_8MIx969y2M9boJkuxPfvdG7Y74T"},{"@type":"CROSSREF","@value":"10.1007/978-4-431-56432-4_14_references_DOI_8MIx969y2M9boJkuxPfvdG7Y74T"}]}