{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360292620026033408.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1006/excr.2001.5295"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014482701952954?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0014482701952954?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"11525641"}}],"dc:title":[{"@value":"Rho Family GTPases Regulate VEGF-Stimulated Endothelial Cell Motility"}],"description":[{"notation":[{"@value":"Migration of endothelial cells induced by vascular endothelial growth factor (VEGF) is a critical step in angiogenesis. Stimulation of motility by growth factors such as VEGF requires interaction with the signal transduction pathways activated by the extracellular matrix (ECM). Here we demonstrate that the Rac GTPase is the critical intersection activated by type 1 collagen ECM and VEGF during stimulation of endothelial cell motility. To analyze the role of the Rho family GTPases in VEGF-stimulated endothelial cell chemotaxis and ECM-stimulated haptotaxis, we transduced the respective fusion proteins in human foreskin dermal endothelial cells using a Tat peptide from the human immunodeficiency virus Tat protein. VEGF signaling required Rac activation during chemotaxis, and Rac and Cdc42 were activated during haptotaxis on type I collagen. Similar to VEGF, Rac activation induced an increase in endothelial cell stress fiber and focal adhesion. Surprisingly, Rho activation was not present in collagen-induced haptotaxis or stimulation of chemotaxis by VEGF, although Rho induced stress fibers and focal adhesions similar to Rac activation. The result of constitutive Rho activation was an inhibition of haptotaxis. Thus, Rac is required and sufficient for the activation of endothelial cell haptotaxis and VEGF-stimulated chemotaxis."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1030285133461061251","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60332714"},{"@type":"NRID","@value":"1000060332714"},{"@type":"NRID","@value":"9000024307022"},{"@type":"NRID","@value":"9000000453863"},{"@type":"NRID","@value":"9000415231883"},{"@type":"NRID","@value":"9000020292519"},{"@type":"NRID","@value":"9000007829124"},{"@type":"NRID","@value":"9000024322435"},{"@type":"NRID","@value":"9000024349096"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/norisoga"}],"foaf:name":[{"@value":"Norihito Soga"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033412","@type":"Researcher","foaf:name":[{"@value":"Noriyuki Namba"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033409","@type":"Researcher","foaf:name":[{"@value":"Sandy McAllister"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033411","@type":"Researcher","foaf:name":[{"@value":"Lynn Cornelius"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033410","@type":"Researcher","foaf:name":[{"@value":"Steven L. Teitelbaum"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033414","@type":"Researcher","foaf:name":[{"@value":"Steven F. Dowdy"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033413","@type":"Researcher","foaf:name":[{"@value":"Juichi Kawamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620026033408","@type":"Researcher","foaf:name":[{"@value":"Keith A. Hruska"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00144827"}],"prism:publicationName":[{"@value":"Experimental Cell Research"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2001-09","prism:volume":"269","prism:number":"1","prism:startingPage":"73","prism:endingPage":"87"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0014482701952954?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0014482701952954?httpAccept=text/plain"}],"createdAt":"2002-09-16","modifiedAt":"2021-05-03","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factor%20A","dc:title":"Vascular Endothelial Growth Factor A"},{"@id":"https://cir.nii.ac.jp/all?q=rho%20GTP-Binding%20Proteins","dc:title":"rho GTP-Binding Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Fusion%20Proteins","dc:title":"Recombinant Fusion Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Fluorescent%20Antibody%20Technique","dc:title":"Fluorescent Antibody Technique"},{"@id":"https://cir.nii.ac.jp/all?q=Neovascularization,%20Physiologic","dc:title":"Neovascularization, Physiologic"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelial%20Growth%20Factors","dc:title":"Endothelial Growth Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Antimalarials","dc:title":"Antimalarials"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Movement","dc:title":"Cell Movement"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=cdc42%20GTP-Binding%20Protein","dc:title":"cdc42 GTP-Binding Protein"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Cytoskeleton","dc:title":"Cytoskeleton"},{"@id":"https://cir.nii.ac.jp/all?q=Lymphokines","dc:title":"Lymphokines"},{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factors","dc:title":"Vascular Endothelial Growth Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Chemotaxis","dc:title":"Chemotaxis"},{"@id":"https://cir.nii.ac.jp/all?q=Chloroquine","dc:title":"Chloroquine"},{"@id":"https://cir.nii.ac.jp/all?q=Actins","dc:title":"Actins"},{"@id":"https://cir.nii.ac.jp/all?q=Vinculin","dc:title":"Vinculin"},{"@id":"https://cir.nii.ac.jp/all?q=Extracellular%20Matrix","dc:title":"Extracellular Matrix"},{"@id":"https://cir.nii.ac.jp/all?q=rac%20GTP-Binding%20Proteins","dc:title":"rac GTP-Binding Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Products,%20tat","dc:title":"Gene Products, tat"},{"@id":"https://cir.nii.ac.jp/all?q=Collagen","dc:title":"Collagen"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelium,%20Vascular","dc:title":"Endothelium, Vascular"},{"@id":"https://cir.nii.ac.jp/all?q=Signal%20Transduction","dc:title":"Signal Transduction"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050856995324520704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283693706165888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Role of Afadin in Vascular Endothelial Growth Factor– and Sphingosine 1-Phosphate–Induced 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