{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360292620144026752.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/gad.853700"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/gad.853700"}}],"dc:title":[{"@value":"Direct protein–protein interaction between the intracellular domain of TRA-2 and the transcription factor TRA-1A modulates feminizing activity in <i>C. elegans</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>In the nematode <jats:italic>Caenorhabditis elegans</jats:italic>, the zinc finger transcriptional regulator TRA-1A directs <jats:italic>XX</jats:italic> somatic cells to adopt female fates. The membrane protein TRA-2A indirectly activates TRA-1A by binding and inhibiting a masculinizing protein, FEM-3. Here we report that a part of the intracellular domain of TRA-2A, distinct from the FEM-3 binding region, directly binds TRA-1A. Overproduction of this TRA-1A-binding region has <jats:italic>tra-1</jats:italic>-dependent feminizing activity in somatic tissues, indicating that the interaction enhances TRA-1A activity. Consistent with this hypothesis, we show that<jats:italic>tra-2(mx)</jats:italic> mutations, which weakly masculinize somatic tissues, disrupt the TRA-2/TRA-1A interaction. Paradoxically,<jats:italic>tra-2(mx)</jats:italic> mutations feminize the <jats:italic>XX</jats:italic> germ line, as do <jats:italic>tra-1</jats:italic> mutations mapping to the TRA-2 binding domain. We propose that these mutations render <jats:italic>tra-2</jats:italic> insensitive to a negative regulator in the <jats:italic>XX</jats:italic> germ line, and we speculate that this regulator targets the TRA-2/TRA-1 complex. The intracellular domain of TRA-2A is likely to be produced as a soluble protein in vivo through proteolytic cleavage of TRA-2A or through translation of an<jats:italic>XX</jats:italic> germ line-specific mRNA. We further show that tagged derivatives of the intracellular domain of TRA-2 localize to the nucleus, supporting the hypothesis that this domain is capable of modulating TRA-1A activity in a manner reminiscent of Notch and Su(H).</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380292620144026624","@type":"Researcher","foaf:name":[{"@value":"David H. Lum"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620144026754","@type":"Researcher","foaf:name":[{"@value":"Patricia E. Kuwabara"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620144026753","@type":"Researcher","foaf:name":[{"@value":"David Zarkower"}]},{"@id":"https://cir.nii.ac.jp/crid/1380292620144026752","@type":"Researcher","foaf:name":[{"@value":"Andrew M. Spence"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08909369"},{"@type":"EISSN","@value":"15495477"}],"prism:publicationName":[{"@value":"Genes & Development"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2000-12-15","prism:volume":"14","prism:number":"24","prism:startingPage":"3153","prism:endingPage":"3165"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/gad.853700"}],"createdAt":"2002-07-27","modifiedAt":"2021-11-15","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050587981433404416","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of mammalian Patched 1."},{"@value":"Human DJ-1-specific Transcriptional Activation of Tyrosine Hydroxylase Gene"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235994038400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A Specific Set of Exon Junction Complex Subunits Is Required for the Nuclear Retention of Unspliced RNAs in <i>Caenorhabditis elegans</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181357329664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The C-terminal cytoplasmic tail of hedgehog receptor Patched1 is a platform for E3 ubiquitin ligase complexes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580232172260224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The germ cell‐specific <scp>TAP</scp>‐like protein <scp>NXF</scp>‐2 forms a novel granular structure and is required for <i>tra‐2</i> 3′<scp>UTR</scp>‐dependent <scp>mRNA</scp> export in <i>Caenorhabditis elegans</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846642316903424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661908525952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Placental labyrinth formation in mice requires endothelial FLRT2–UNC5B signaling"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1101/gad.853700"},{"@type":"CROSSREF","@value":"10.1007/s11010-015-2643-4_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0018638_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"},{"@type":"CROSSREF","@value":"10.1111/gtc.12978_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"},{"@type":"CROSSREF","@value":"10.1083/jcb.201209151_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"},{"@type":"CROSSREF","@value":"10.1242/dev.149757_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"},{"@type":"CROSSREF","@value":"10.1128/mcb.01298-12_references_DOI_Zq0lGYqa7bHzC3xOozMqdSfUEhQ"}]}