{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360294643854079232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.virusres.2021.198492"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0168170221001994?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0168170221001994?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"34174342"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Ibaraki virus enters host cells by macropinocytosis"}],"description":[{"notation":[{"@value":"Ibaraki virus (IBAV) is the pathogen associated with Ibaraki disease. In a previous study, we suggested that IBAV enters hamster lung (HmLu-1) cells via endocytosis and subsequently escapes into the cytoplasm upon endosomal acidification. However, it is unclear which of the endocytic pathways IBAV utilizes. In this study, we aimed to further elucidate the pathway of IBAV entry into host cells. We found that IBAV replication was not suppressed by inhibitors of clathrin-mediated or caveolin-mediated endocytosis but was markedly suppressed by 5-(N-ethyl-N-isopropyl) amiloride (EIPA) and cytochalasin D, both of which inhibit macropinocytosis. Monensin, which inhibits endosomal acidification, also suppressed IBAV replication. To assess the inhibitory effects of these reagents on endocytosis, dextran and transferrin were used as indicators of macropinocytosis and clathrin-mediated endocytic activity, respectively. Our data confirmed that EIPA and monensin inhibited dextran uptake, and cytochalasin D inhibited the uptake of both. Additionally, we confirmed that endosomal/lysosomal acidification was inhibited by monensin. These results suggest that the macropinocytosis pathway is the major route of IBAV entry and confirm that IBAV infection of HmLu-1 cells is dependent on endosomal acidification."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380294643854079489","@type":"Researcher","foaf:name":[{"@value":"Yuki Maeda"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294643854079373","@type":"Researcher","foaf:name":[{"@value":"Shusaku Shibutani"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294643854079368","@type":"Researcher","foaf:name":[{"@value":"Keiko Onishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380294643854079370","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Iwata"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01681702"}],"prism:publicationName":[{"@value":"Virus Research"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2021-09","prism:volume":"302","prism:startingPage":"198492"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license","https://doi.org/10.15223/policy-017","https://doi.org/10.15223/policy-037","https://doi.org/10.15223/policy-012","https://doi.org/10.15223/policy-029","https://doi.org/10.15223/policy-004"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0168170221001994?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0168170221001994?httpAccept=text/plain"}],"createdAt":"2021-06-24","modifiedAt":"2025-09-29","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cytochalasin%20D","dc:title":"Cytochalasin D"},{"@id":"https://cir.nii.ac.jp/all?q=Dextrans","dc:title":"Dextrans"},{"@id":"https://cir.nii.ac.jp/all?q=Virus%20Internalization","dc:title":"Virus Internalization"},{"@id":"https://cir.nii.ac.jp/all?q=Clathrin","dc:title":"Clathrin"},{"@id":"https://cir.nii.ac.jp/all?q=Endocytosis","dc:title":"Endocytosis"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Cricetinae","dc:title":"Cricetinae"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Pinocytosis","dc:title":"Pinocytosis"},{"@id":"https://cir.nii.ac.jp/all?q=Monensin","dc:title":"Monensin"},{"@id":"https://cir.nii.ac.jp/all?q=Orbivirus","dc:title":"Orbivirus"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040850857185536640","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21K05957"},{"@type":"JGN","@value":"JP21K05957"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K05957/"}],"notation":[{"@language":"ja","@value":"dsRNAレオウイルスの複製機構の解明から創薬開発への基盤研究"},{"@language":"en","@value":"Studies on analysis of replication system on dsRNA virus and development of its drugs"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145838086528","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Virus entry by macropinocytosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013172121252736","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Na+/H+ Exchanger NHE6 Modulates Endosomal pH to Control Processing of Amyloid Precursor Protein in a Cell Culture Model of Alzheimer Disease"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013172248966400","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Alteration of intracellular traffic by monensin; 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