{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360298344440828288.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1046/j.1365-2818.1998.00336.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2818.1998.00336.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2818.1998.00336.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2818.1998.00336.x"}}],"dc:title":[{"@value":"Theory of confocal fluorescence imaging in the programmable array microscope (PAM)"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The programmable array microscope (PAM) uses a spatial light modulator (SLM) to generate an arbitrary pattern of conjugate illumination and detection elements. The SLM dissects the fluorescent light imaged by the objective into a focal conjugate image, <jats:italic>I</jats:italic><jats:sub>c</jats:sub>, formed by the ‘in‐focus’ light, and a nonconjugate image, <jats:italic>I</jats:italic><jats:sub>nc</jats:sub>, formed by the ‘out‐of‐focus’ light. We discuss two different schemes for confocal imaging using the PAM. In the first, a grid of points is shifted to scan the complete image. The second, faster approach, uses a short tiled pseudorandom sequence of two‐dimensional patterns. In the first case, <jats:italic>I</jats:italic><jats:sub>c</jats:sub> is analogous to a confocal image and <jats:italic>I</jats:italic><jats:sub>nc</jats:sub> to a conventional image minus <jats:italic>I</jats:italic><jats:sub>c</jats:sub>. In the second case <jats:italic>I</jats:italic><jats:sub>c</jats:sub> and <jats:italic>I</jats:italic><jats:sub>nc</jats:sub> are the sum and the difference, respectively, of a conventional and a confocal image. The pseudorandom sequence approach requires post‐processing to retrieve the confocal part, but generates significantly higher signal levels for an equivalent integration time.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380298344440828292","@type":"Researcher","foaf:name":[{"@value":"Verveer"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344440828288","@type":"Researcher","foaf:name":[{"@value":"Hanley"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344440828289","@type":"Researcher","foaf:name":[{"@value":"Verbeek"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344440828291","@type":"Researcher","foaf:name":[{"@value":"Van Vliet"}]},{"@id":"https://cir.nii.ac.jp/crid/1380298344440828290","@type":"Researcher","foaf:name":[{"@value":"Jovin"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00222720"},{"@type":"EISSN","@value":"13652818"}],"prism:publicationName":[{"@value":"Journal of Microscopy"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1998-03","prism:volume":"189","prism:number":"3","prism:startingPage":"192","prism:endingPage":"198"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2818.1998.00336.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2818.1998.00336.x"},{"@id":"https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2818.1998.00336.x"}],"createdAt":"2003-03-12","modifiedAt":"2023-09-11","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360567185380883712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Closed-Loop Feedback Illumination for Optical Inverse Tone-Mapping in Light Microscopy"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1046/j.1365-2818.1998.00336.x"},{"@type":"CROSSREF","@value":"10.1109/tvcg.2010.104_references_DOI_W04r1yTmt017rNRAcnzzk0kM0hx"}]}