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Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons
Bibliographic Information
- Published
- 2022-08-06
- Resource Type
- journal article
- Rights Information
-
- https://www.springer.com/tdm
- https://www.springer.com/tdm
- DOI
-
- 10.1007/s00795-022-00330-z
- Publisher
- Springer Science and Business Media LLC
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Description
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
Journal
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- Medical Molecular Morphology
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Medical Molecular Morphology 55 (4), 292-303, 2022-08-06
Springer Science and Business Media LLC