{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360307812502117632.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s11033-024-09367-y"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/content/pdf/10.1007/s11033-024-09367-y.pdf"}},{"identifier":{"@type":"URI","@value":"https://link.springer.com/article/10.1007/s11033-024-09367-y/fulltext.html"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Increase in the solubility of uvsY using a site saturation mutagenesis library for application in a lyophilized reagent for recombinase polymerase amplification"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n          <jats:sec>\n            <jats:title>Background</jats:title>\n            <jats:p>Recombinase uvsY from bacteriophage T4, along with uvsX, is a key enzyme for recombinase polymerase amplification (RPA), which is used to amplify a target DNA sequence at a constant temperature. uvsY, though essential, poses solubility challenges, complicating the lyophilization of RPA reagents. This study aimed to enhance uvsY solubility.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Methods</jats:title>\n            <jats:p>Our hypothesis centered on the C-terminal region of uvsY influencing solubility. To test this, we generated a site-saturation mutagenesis library for amino acid residues Lys91–Glu134 of the N-terminal (His)<jats:sub>6</jats:sub>-tagged uvsY.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Results</jats:title>\n            <jats:p>Screening 480 clones identified A116H as the variant with superior solubility. Lyophilized RPA reagents featuring the uvsY variant A116H demonstrated enhanced performance compared to those with wild-type uvsY.</jats:p>\n          </jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions</jats:title>\n            <jats:p>The uvsY variant A116H emerges as an appealing choice for RPA applications, offering improved solubility and heightened lyophilization feasibility.</jats:p>\n          </jats:sec>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380307812502117639","@type":"Researcher","foaf:name":[{"@value":"Kenta Morimoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117642","@type":"Researcher","foaf:name":[{"@value":"Kevin Maafu Juma"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117661","@type":"Researcher","foaf:name":[{"@value":"Masaya Yamagata"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117662","@type":"Researcher","foaf:name":[{"@value":"Teisuke Takita"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117651","@type":"Researcher","foaf:name":[{"@value":"Kenji Kojima"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117646","@type":"Researcher","foaf:name":[{"@value":"Koichiro Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380307812502117650","@type":"Researcher","foaf:name":[{"@value":"Itaru Yanagihara"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801191453952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90263219"},{"@type":"NRID","@value":"1000090263219"},{"@type":"NRID","@value":"9000003008736"},{"@type":"NRID","@value":"9000003053320"},{"@type":"NRID","@value":"9000004120142"},{"@type":"NRID","@value":"9000018311525"},{"@type":"NRID","@value":"9000363296744"},{"@type":"NRID","@value":"9000391930375"},{"@type":"NRID","@value":"9000391920282"},{"@type":"NRID","@value":"9000258425621"},{"@type":"NRID","@value":"9000402447088"},{"@type":"NRID","@value":"9000342803465"},{"@type":"NRID","@value":"9000019094840"},{"@type":"NRID","@value":"9000291272861"},{"@type":"NRID","@value":"9000363283839"},{"@type":"NRID","@value":"9000239879073"},{"@type":"NRID","@value":"9000391928805"},{"@type":"NRID","@value":"9000401962575"},{"@type":"NRID","@value":"9000290380514"},{"@type":"NRID","@value":"9000391943585"},{"@type":"NRID","@value":"9000391936788"},{"@type":"NRID","@value":"9000401961258"},{"@type":"NRID","@value":"9000388490773"},{"@type":"NRID","@value":"9000291272866"},{"@type":"NRID","@value":"9000241149838"},{"@type":"NRID","@value":"9000401542637"},{"@type":"NRID","@value":"9000401545469"},{"@type":"NRID","@value":"9000004415412"},{"@type":"NRID","@value":"9000004530684"},{"@type":"NRID","@value":"9000019094831"},{"@type":"NRID","@value":"9000363296962"},{"@type":"NRID","@value":"9000391924093"},{"@type":"NRID","@value":"9000253375476"},{"@type":"NRID","@value":"9000008497057"},{"@type":"NRID","@value":"9000254571110"},{"@type":"NRID","@value":"9000006238493"},{"@type":"NRID","@value":"9000316619983"},{"@type":"NRID","@value":"9000020144373"},{"@type":"NRID","@value":"9000363285851"},{"@type":"NRID","@value":"9000363284810"},{"@type":"NRID","@value":"9000391968071"},{"@type":"NRID","@value":"9000391928747"},{"@type":"NRID","@value":"9000258172127"},{"@type":"NRID","@value":"9000363284670"},{"@type":"NRID","@value":"9000004530687"},{"@type":"NRID","@value":"9000363300253"},{"@type":"NRID","@value":"9000309571758"},{"@type":"NRID","@value":"9000252815346"},{"@type":"NRID","@value":"9000401542207"},{"@type":"NRID","@value":"9000401868040"},{"@type":"NRID","@value":"9000401724114"},{"@type":"NRID","@value":"9000008500698"},{"@type":"NRID","@value":"9000399598377"},{"@type":"NRID","@value":"9000006335514"},{"@type":"NRID","@value":"9000023668278"},{"@type":"NRID","@value":"9000023652722"},{"@type":"NRID","@value":"9000242899487"},{"@type":"NRID","@value":"9000391951269"},{"@type":"NRID","@value":"9000391929199"},{"@type":"NRID","@value":"9000383709298"},{"@type":"NRID","@value":"9000402237271"},{"@type":"NRID","@value":"9000107307703"},{"@type":"NRID","@value":"9000020140714"},{"@type":"NRID","@value":"9000018867362"},{"@type":"NRID","@value":"9000391950788"},{"@type":"NRID","@value":"9000252814746"},{"@type":"NRID","@value":"9000002493380"},{"@type":"NRID","@value":"9000363297075"},{"@type":"NRID","@value":"9000363296830"},{"@type":"NRID","@value":"9000239877807"},{"@type":"NRID","@value":"9000253183268"},{"@type":"NRID","@value":"9000401545824"},{"@type":"NRID","@value":"9000401962711"},{"@type":"NRID","@value":"9000258425253"},{"@type":"NRID","@value":"9000413537441"},{"@type":"NRID","@value":"9000243875824"},{"@type":"NRID","@value":"9000363297049"},{"@type":"NRID","@value":"9000363296959"},{"@type":"NRID","@value":"9000303640570"},{"@type":"NRID","@value":"9000391918594"},{"@type":"NRID","@value":"9000391923477"},{"@type":"NRID","@value":"9000401959165"},{"@type":"NRID","@value":"9000291272781"},{"@type":"NRID","@value":"9000401545629"},{"@type":"NRID","@value":"9000401963052"},{"@type":"NRID","@value":"9000401959665"},{"@type":"NRID","@value":"9000412335066"},{"@type":"NRID","@value":"9000363283958"},{"@type":"NRID","@value":"9000309558350"},{"@type":"NRID","@value":"9000391923922"},{"@type":"NRID","@value":"9000391931114"},{"@type":"NRID","@value":"9000252814768"},{"@type":"NRID","@value":"9000363897615"},{"@type":"NRID","@value":"9000367821496"},{"@type":"NRID","@value":"9000381068910"},{"@type":"NRID","@value":"9000363296814"},{"@type":"NRID","@value":"9000391950953"},{"@type":"NRID","@value":"9000391919795"},{"@type":"NRID","@value":"9000253183772"},{"@type":"NRID","@value":"9000317549422"},{"@type":"NRID","@value":"9000363274544"},{"@type":"NRID","@value":"9000363283641"},{"@type":"NRID","@value":"9000363285844"},{"@type":"NRID","@value":"9000363274696"},{"@type":"NRID","@value":"9000363282670"},{"@type":"NRID","@value":"9000363274816"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0185266"}],"foaf:name":[{"@value":"Shinsuke 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