{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360565166553068416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/ac401188f"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/ac401188f"}},{"identifier":{"@type":"PMID","@value":"23802134"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Correlation of Carotenoid Accumulation with Aggregation and Biofilm Development in Rhodococcus sp. SD-74"}],"description":[{"notation":[{"@value":"Aggregation of bacterial populations substantially influences their characteristic properties and functions compared with the planktonic counterpart. It is also involved in the initial stages of biofilm development. Many studies have revealed important roles of bacterial aggregation in microbial production and biodegradation. Nevertheless, mechanistic understanding of bacterial aggregation in vivo and at the molecular level is far from complete. Here, we present a noninvasive, label-free Raman microspectroscopic approach to investigate the aggregation and biofilm development of the biotechnologically important Rhodococcus sp. SD-74. We found that the concentration of intracellular carotenoids increases more than 3-fold within 1 week as the biofilm develops. Raman imaging experiments confirmed that the carotenoid accumulation occurs throughout the Rhodococcus sp. SD-74 biofilm. The correlation between the carotenoid Raman intensities and biofilm development found in the present study provides a new means for quantitative, molecular-level assessment of the level of biofilm development, which is not possible with dye staining assay or electron microscopy. Moreover, our results suggest that microbial production of carotenoids in pigmented bacteria such as Rhodococcus sp. SD-74 may potentially be controlled via bacterial aggregation and biofilm formation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380847869715296512","@type":"Researcher","foaf:name":[{"@value":"Yi-Ting Zheng"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry\rand Institute of Molecular Science, National Chiao Tung University, Hsinchu 30010, Taiwan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276184318208","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30644827"},{"@type":"NRID","@value":"1000030644827"},{"@type":"NRID","@value":"9000363297356"},{"@type":"NRID","@value":"9000006356226"},{"@type":"NRID","@value":"9000018461402"},{"@type":"NRID","@value":"9000364764613"},{"@type":"NRID","@value":"9000364764489"},{"@type":"NRID","@value":"9000363281735"},{"@type":"NRID","@value":"9000293834270"},{"@type":"NRID","@value":"9000413498799"},{"@type":"NRID","@value":"9000413479340"},{"@type":"NRID","@value":"9000413479342"},{"@type":"NRID","@value":"9000413518101"},{"@type":"NRID","@value":"9000413518121"},{"@type":"NRID","@value":"9000364764873"},{"@type":"NRID","@value":"9000364764135"},{"@type":"NRID","@value":"9000364764740"},{"@type":"NRID","@value":"9000363296152"},{"@type":"NRID","@value":"9000363296149"},{"@type":"NRID","@value":"9000263058009"},{"@type":"NRID","@value":"9000413497652"},{"@type":"NRID","@value":"9000364764760"},{"@type":"NRID","@value":"9000363297016"},{"@type":"NRID","@value":"9000364764667"},{"@type":"NRID","@value":"9000363296536"},{"@type":"NRID","@value":"9000363297359"},{"@type":"NRID","@value":"9000364764382"},{"@type":"NRID","@value":"9000364764268"},{"@type":"NRID","@value":"9000364764768"},{"@type":"NRID","@value":"9000364764448"},{"@type":"NRID","@value":"9000364764424"},{"@type":"NRID","@value":"9000363296954"},{"@type":"NRID","@value":"9000368937495"},{"@type":"NRID","@value":"9000367422068"},{"@type":"NRID","@value":"9000364764568"},{"@type":"NRID","@value":"9000364764871"},{"@type":"NRID","@value":"9000363296879"},{"@type":"NRID","@value":"9000398394369"},{"@type":"NRID","@value":"9000243768188"},{"@type":"NRID","@value":"9000248248842"},{"@type":"NRID","@value":"9000364763930"},{"@type":"NRID","@value":"9000364763799"},{"@type":"NRID","@value":"9000413497737"},{"@type":"NRID","@value":"9000364764819"},{"@type":"NRID","@value":"9000364763910"},{"@type":"NRID","@value":"9000364764770"},{"@type":"NRID","@value":"9000364764432"},{"@type":"NRID","@value":"9000364764870"},{"@type":"NRID","@value":"9000364764736"},{"@type":"NRID","@value":"9000364764717"},{"@type":"NRID","@value":"9000415048824"},{"@type":"NRID","@value":"9000413512200"},{"@type":"NRID","@value":"9000413512198"},{"@type":"NRID","@value":"9000364764725"},{"@type":"NRID","@value":"9000364764716"},{"@type":"NRID","@value":"9000364764779"},{"@type":"NRID","@value":"9000364764765"},{"@type":"NRID","@value":"9000363296407"},{"@type":"NRID","@value":"9000347296546"},{"@type":"NRID","@value":"9000404102685"},{"@type":"NRID","@value":"9000364764025"},{"@type":"NRID","@value":"9000364764636"},{"@type":"NRID","@value":"9000364764575"},{"@type":"NRID","@value":"9000364764869"},{"@type":"NRID","@value":"9000364764784"},{"@type":"NRID","@value":"9000363294714"},{"@type":"NRID","@value":"9000364764761"},{"@type":"NRID","@value":"9000364764675"},{"@type":"NRID","@value":"9000364764866"},{"@type":"NRID","@value":"9000364764608"},{"@type":"NRID","@value":"9000364764685"},{"@type":"NRID","@value":"9000243876847"},{"@type":"NRID","@value":"9000399411669"},{"@type":"NRID","@value":"9000398385680"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/masanori_toyofuku"}],"foaf:name":[{"@value":"Masanori Toyofuku"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Life and\rEnvironmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847869715296515","@type":"Researcher","foaf:name":[{"@value":"Nobuhiko Nomura"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Life and\rEnvironmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847869715296514","@type":"Researcher","foaf:name":[{"@value":"Shinsuke Shigeto"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry\rand Institute of Molecular Science, National Chiao Tung University, Hsinchu 30010, Taiwan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00032700"},{"@type":"EISSN","@value":"15206882"}],"prism:publicationName":[{"@value":"Analytical Chemistry"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2013-07-10","prism:volume":"85","prism:number":"15","prism:startingPage":"7295","prism:endingPage":"7301"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/ac401188f"}],"createdAt":"2013-06-26","modifiedAt":"2023-04-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Biodegradation,%20Environmental","dc:title":"Biodegradation, Environmental"},{"@id":"https://cir.nii.ac.jp/all?q=Biofilms","dc:title":"Biofilms"},{"@id":"https://cir.nii.ac.jp/all?q=Intracellular%20Space","dc:title":"Intracellular Space"},{"@id":"https://cir.nii.ac.jp/all?q=Rhodococcus","dc:title":"Rhodococcus"},{"@id":"https://cir.nii.ac.jp/all?q=Plankton","dc:title":"Plankton"},{"@id":"https://cir.nii.ac.jp/all?q=Carotenoids","dc:title":"Carotenoids"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782269220864","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25701012"},{"@type":"JGN","@value":"JP25701012"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25701012/"}],"notation":[{"@language":"ja","@value":"ベシクルを介した微生物間ネットワークの解明とそのデザイン方法の創出"},{"@language":"en","@value":"Bacterial networking though membrane vesicles"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050292815318941184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms"}]},{"@id":"https://cir.nii.ac.jp/crid/1050304183894742784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in Single Aurantiochytrium limacinum Cells with Linear and Nonlinear Raman Microspectroscopy"},{"@value":"Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in Single <i>Aurantiochytrium limacinum</i> Cells with Linear and Nonlinear Raman Microspectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143673377664","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Assignment and anharmonicity analysis of overtone and combination bands observed in the resonance Raman spectra of carotenoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145200439040","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145425434112","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Alkane hydroxylase homologues in Gram‐positive strains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145842074368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Label-Free in Situ SERS Imaging of Biofilms"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011146525981184","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"FT-INFRARED AND FT-RAMAN SPECTROSCOPY IN BIOMEDICAL RESEARCH"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621439492480","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Understanding biofilm resistance to antibacterial agents"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643702687488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Machine learning-assisted single-cell Raman fingerprinting for in situ and nondestructive classification of prokaryotes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094940242176","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Can dietary beta-carotene materially reduce human cancer rates?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855571099547904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Chemical interactions in the aggregation of bacteria bioflocculation in waste treatment"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044099070336","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Intracellular Chemical Imaging of Heme-Containing Enzymes Involved in Innate Immunity Using Resonance Raman Microscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137044704877440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Novel β-carotene ketolases from non-photosynthetic bacteria for canthaxanthin synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045730793856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Label-free Raman observation of cytochrome c dynamics during apoptosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518753232256","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Endogenous oxidative stress produces diversity and adaptability in biofilm communities"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520966505472","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Evidence for interfacial uptake in hexadecane degradation by Rhodococcus equi: the importance of cell flocculation"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699994735486848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Combined use of confocal laser scanning microscopy (CLSM) and Raman microscopy (RM): Investigations on EPS – Matrix"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995594896000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Leucine pools in <i>Escherichia coli</i> biofilm discovered by Raman imaging"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995916419584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Disentangling Dynamic Changes of Multiple Cellular Components during the Yeast Cell Cycle by <i>in Vivo</i> Multivariate Raman Imaging"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699996274940672","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"MICROBIAL BIOFILMS"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468633198464","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The developmental model of microbial biofilms: ten years of a paradigm up for review"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468914843520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Raman spectra of carotenoids in natural products"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470146788352","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Raman spectroscopic study of the heterogeneity of microcolonies of a pigmented bacterium"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470167967744","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Confocal Raman microspectroscopy as a tool for studying the chemical heterogeneities of biofilms in situ"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471423155328","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Raman and Infrared Microspectral Imaging of Mitotic Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943574685312","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Physiological heterogeneity in biofilms"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262943606361728","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Dietary Carotenoids and Risk of Lung Cancer in a Pooled Analysis of Seven Cohort Studies"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262946357665024","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Identification of medically relevant microorganisms by vibrational spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544418743976320","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Preresonance Raman spectra of crystals of retinal isomers"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894604583808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Metabolic engineering for synthesis of aryl carotenoids in Rhodococcus"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896031890048","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Normal‐coordinate analysis of β‐carotene isomers and assignments of the Raman and infrared bands"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368638891008","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Dietary Carotenoids, Vitamins A, C, and E, and Advanced Age-Related Macular Degeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368965006848","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"In vivo multimode Raman imaging reveals concerted molecular composition and distribution changes during yeast cell cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370654963200","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Environmental and cultural stimulants in the production of carotenoids from microorganisms"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370781275136","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The genus Rhodococcus"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845862113792","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular Basis of In Vivo Biofilm Formation by Bacterial Pathogens"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846159354880","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Biochemical and genetic characteristics of Cronobacter sakazakii biofilm formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318694773888","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nonresonant Confocal Raman Imaging of DNA and Protein Distribution in Apoptotic Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793486180096","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular-Level Investigation of the Structure, Transformation, and Bioactivity of Single Living Fission Yeast Cells by Time- and Space-Resolved Raman Spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793619959168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Bacterial metabolism of long-chain n-alkanes"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233271039717248","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Resonance Raman studies of the conformations of all-Trans carotenoids in light-harvesting systems of photosynthetic bacteria"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001288063069184","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"複雑分子系の挙動に迫る時空間分解振動分光研究"},{"@language":"en","@value":"Time- and Space-Resolved Vibrational Spectroscopic Approaches to Elucidate the Behaviors of Complex Molecular Systems: From Biological Cells to Hybrid Solar-Cell Materials"}]},{"@id":"https://cir.nii.ac.jp/crid/1390291767896737920","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Raman Micro­spectroscopy and Imaging of Filamentous Fungi"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1021/ac401188f"},{"@type":"KAKEN","@value":"PRODUCT-15133831"},{"@type":"OPENAIRE","@value":"doi_dedup___::ef89f7933eb873ca9b44bf1d24385abc"},{"@type":"CROSSREF","@value":"10.3175/molsci.12.a0099_references_DOI_9RSsfJPhYkh7ogE0UTYcTB6dlvw"},{"@type":"CROSSREF","@value":"10.1016/j.isci.2021.102975_references_DOI_9RSsfJPhYkh7ogE0UTYcTB6dlvw"},{"@type":"CROSSREF","@value":"10.1264/jsme2.me22006_references_DOI_9RSsfJPhYkh7ogE0UTYcTB6dlvw"},{"@type":"CROSSREF","@value":"10.1038/s41598-020-64737-3_references_DOI_9RSsfJPhYkh7ogE0UTYcTB6dlvw"},{"@type":"CROSSREF","@value":"10.1021/acs.jpcb.3c00302_references_DOI_9RSsfJPhYkh7ogE0UTYcTB6dlvw"}]}