{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360567181955146752.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.bbrc.2016.10.154"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X16317922?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0006291X16317922?httpAccept=text/xml"}},{"identifier":{"@type":"PMID","@value":"27818200"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Hypoxia-induced production of peptidylarginine deiminases and citrullinated proteins in malignant glioma cells"}],"description":[{"notation":[{"@value":"Recently, it has been reported that hypoxia highly enhances expression of peptidylarginine deiminase (PAD) 4 and production of citrullinated proteins in some tumor cells. However, little is known about malignant gliomas on this issue. Therefore, we here investigated whether expression of PADs was induced by hypoxia and whether PADs citrullinated intracellular proteins if induced using U-251 MG cells of a human malignant glioma cell line.Expression of PADs in U-251 MG cells, cultured under hypoxia or normoxia for 24 h, was investigated by quantitative polymerase chain reaction (qPCR). Citrullination of proteins in the cells and the cell lysates incubated for 48 h with or without CaThe mRNA levels of PAD1, 2, 3, and 4 were up-regulated by hypoxia in a hypoxia-inducible factor-1-dependent manner in U-251 MG cells. In spite of the increased expression, intracellular proteins were not citrullinated. However, the induced PADs citrullinated U-251 MG cell-derived proteins when the cells were lysed. Multiple proteins citrullinated by hypoxia-induced PADs were identified. In addition, the extracellular domain of vascular endothelial growth factor receptor 2 was citrullinated by human PAD2 in vitro.Our data may contribute to understanding of pathophysiology of malignant gliomas from the aspects of protein citrullination."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380567181955146756","@type":"Researcher","foaf:name":[{"@value":"Taigen Sase"}]},{"@id":"https://cir.nii.ac.jp/crid/1420001326205224064","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00509911"},{"@type":"NRID","@value":"1000000509911"},{"@type":"NRID","@value":"9000399374185"},{"@type":"NRID","@value":"9000398610011"},{"@type":"NRID","@value":"9000398611357"},{"@type":"NRID","@value":"9000398276106"},{"@type":"NRID","@value":"9000399221036"},{"@type":"NRID","@value":"9000263063203"},{"@type":"NRID","@value":"9000018237421"},{"@type":"NRID","@value":"9000391979466"},{"@type":"NRID","@value":"9000347091174"},{"@type":"NRID","@value":"9000398610461"},{"@type":"NRID","@value":"9000398275546"},{"@type":"NRID","@value":"9000014204534"},{"@type":"NRID","@value":"9000391976764"},{"@type":"NRID","@value":"9000283604086"},{"@type":"NRID","@value":"9000391977075"},{"@type":"NRID","@value":"9000391975890"},{"@type":"NRID","@value":"9000314067652"},{"@type":"NRID","@value":"9000347091129"},{"@type":"NRID","@value":"9000398395714"},{"@type":"NRID","@value":"9000399375323"},{"@type":"NRID","@value":"9000399221450"},{"@type":"NRID","@value":"9000347091383"},{"@type":"NRID","@value":"9000347090988"},{"@type":"NRID","@value":"9000347092947"},{"@type":"NRID","@value":"9000402599983"},{"@type":"NRID","@value":"9000347092743"},{"@type":"NRID","@value":"9000347091585"},{"@type":"NRID","@value":"9000399373912"},{"@type":"NRID","@value":"9000347093999"},{"@type":"NRID","@value":"9000283604011"},{"@type":"NRID","@value":"9000399375737"},{"@type":"NRID","@value":"9000399378533"},{"@type":"NRID","@value":"9000399375069"},{"@type":"NRID","@value":"9000399374337"},{"@type":"NRID","@value":"9000318597141"},{"@type":"NRID","@value":"9000391966352"},{"@type":"NRID","@value":"9000391966538"},{"@type":"NRID","@value":"9000398610711"},{"@type":"NRID","@value":"9000399373743"},{"@type":"NRID","@value":"9000347093373"},{"@type":"NRID","@value":"9000398607627"},{"@type":"NRID","@value":"9000391976935"},{"@type":"NRID","@value":"9000347091094"},{"@type":"NRID","@value":"9000347093269"},{"@type":"NRID","@value":"9000347091744"},{"@type":"NRID","@value":"9000347092342"},{"@type":"NRID","@value":"9000399375550"},{"@type":"NRID","@value":"9000391969466"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/arilayn"}],"foaf:name":[{"@value":"Mitsumi Arito"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146759","@type":"Researcher","foaf:name":[{"@value":"Hidetaka Onodera"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146754","@type":"Researcher","foaf:name":[{"@value":"Kazuki Omoteyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146753","@type":"Researcher","foaf:name":[{"@value":"Manae S. Kurokawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146755","@type":"Researcher","foaf:name":[{"@value":"Yayoi Kagami"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751151502848","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50270658"},{"@type":"NRID","@value":"1000050270658"},{"@type":"NRID","@value":"9000018237398"},{"@type":"NRID","@value":"9000020228065"},{"@type":"NRID","@value":"9000392067742"},{"@type":"NRID","@value":"9000262367021"},{"@type":"NRID","@value":"9000329008464"},{"@type":"NRID","@value":"9000273022363"},{"@type":"NRID","@value":"9000010006231"},{"@type":"NRID","@value":"9000409483484"},{"@type":"NRID","@value":"9000004256492"},{"@type":"NRID","@value":"9000413473047"},{"@type":"NRID","@value":"9000398273419"},{"@type":"NRID","@value":"9000402297871"},{"@type":"NRID","@value":"9000297487273"},{"@type":"NRID","@value":"9000002035407"},{"@type":"NRID","@value":"9000243889454"},{"@type":"NRID","@value":"9000392064366"},{"@type":"NRID","@value":"9000392063949"},{"@type":"NRID","@value":"9000283497119"},{"@type":"NRID","@value":"9000018244181"},{"@type":"NRID","@value":"9000273022285"},{"@type":"NRID","@value":"9000256126648"},{"@type":"NRID","@value":"9000021929429"},{"@type":"NRID","@value":"9000020086690"},{"@type":"NRID","@value":"9000317564710"},{"@type":"NRID","@value":"9000409999264"},{"@type":"NRID","@value":"9000403419771"},{"@type":"NRID","@value":"9000273022227"},{"@type":"NRID","@value":"9000258251863"},{"@type":"NRID","@value":"9000263040273"},{"@type":"NRID","@value":"9000001252745"},{"@type":"NRID","@value":"9000413251991"},{"@type":"NRID","@value":"9000409492261"},{"@type":"NRID","@value":"9000399235027"},{"@type":"NRID","@value":"9000311513166"},{"@type":"NRID","@value":"9000345238716"},{"@type":"NRID","@value":"9000242070932"},{"@type":"NRID","@value":"9000413567451"},{"@type":"NRID","@value":"9000412606102"},{"@type":"NRID","@value":"9000410312742"},{"@type":"NRID","@value":"9000403155173"},{"@type":"NRID","@value":"9000262343329"},{"@type":"NRID","@value":"9000404619352"},{"@type":"NRID","@value":"9000021818394"},{"@type":"NRID","@value":"9000409999436"},{"@type":"NRID","@value":"9000283614349"},{"@type":"NRID","@value":"9000411531147"},{"@type":"NRID","@value":"9000011209222"},{"@type":"NRID","@value":"9000409172995"},{"@type":"NRID","@value":"9000345408753"},{"@type":"NRID","@value":"9000399234982"},{"@type":"NRID","@value":"9000257907086"},{"@type":"NRID","@value":"9000272995038"},{"@type":"NRID","@value":"9000404514418"},{"@type":"NRID","@value":"9000399480795"},{"@type":"NRID","@value":"9000273022432"},{"@type":"NRID","@value":"9000019947647"},{"@type":"NRID","@value":"9000288542480"},{"@type":"NRID","@value":"9000413251707"},{"@type":"NRID","@value":"9000414834085"},{"@type":"NRID","@value":"9000404096029"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0159340"}],"foaf:name":[{"@value":"Akihito Ishigami"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146752","@type":"Researcher","foaf:name":[{"@value":"Yuichiro Tanaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567181955146760","@type":"Researcher","foaf:name":[{"@value":"Tomohiro Kato"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0006291X"}],"prism:publicationName":[{"@value":"Biochemical and Biophysical Research Communications"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2017-01","prism:volume":"482","prism:number":"1","prism:startingPage":"50","prism:endingPage":"56"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0006291X16317922?httpAccept=text/plain"},{"@id":"https://api.elsevier.com/content/article/PII:S0006291X16317922?httpAccept=text/xml"}],"createdAt":"2016-11-03","modifiedAt":"2020-09-27","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Hydrolases","dc:title":"Hydrolases"},{"@id":"https://cir.nii.ac.jp/all?q=Glioma","dc:title":"Glioma"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Enzymologic","dc:title":"Gene Expression Regulation, Enzymologic"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Neoplastic","dc:title":"Gene Expression Regulation, Neoplastic"},{"@id":"https://cir.nii.ac.jp/all?q=Oxygen","dc:title":"Oxygen"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Protein-Arginine%20Deiminase%20Type%202","dc:title":"Protein-Arginine Deiminase Type 2"},{"@id":"https://cir.nii.ac.jp/all?q=Protein-Arginine%20Deiminases","dc:title":"Protein-Arginine Deiminases"},{"@id":"https://cir.nii.ac.jp/all?q=Citrulline","dc:title":"Citrulline"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Hypoxia","dc:title":"Tumor Hypoxia"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781916000384","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16K10228"},{"@type":"JGN","@value":"JP16K10228"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K10228/"}],"notation":[{"@language":"ja","@value":"レビー小体型認知症における新規血清ペプチドバイオマーカーの実用化および治療応用"},{"@language":"en","@value":"Toward clinical application of novel serum peptide biomarkers for dementia with Lewy bodies"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256836100608","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15K15104"},{"@type":"JGN","@value":"JP15K15104"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K15104/"}],"notation":[{"@language":"ja","@value":"シトルリン化蛋白質を指標としたアルツハイマー病早期臨床検査試薬の開発"},{"@language":"en","@value":"Development of early clinical reagents for Alzheimer's disease using citrullinated proteins as index"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360001114011305216","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Peptidylarginine Deiminase Isoforms 1–3 Are Expressed in the Epidermis and Involved in the Deimination of K1 and Filaggrin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232214190464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Anti-vascular endothelial growth factor receptor (VEGFR) 2 autoantibody identification in glioblastoma patient using single B cell-based antibody gene cloning"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011143605546624","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Targeting HIF-1 for cancer therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144570991616","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Abnormal accumulation of citrullinated proteins 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acetyl-proteomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1370850475165518088","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The diagnostic accuracy of anti-citrulline antibody assessment in the diagnosis of patients suspected of rheumatoid arthritis by a general practitioner"}]},{"@id":"https://cir.nii.ac.jp/crid/1370850475165518098","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of edaravone on hypoxic human astrocytes revealed by a proteomic approach"}]},{"@id":"https://cir.nii.ac.jp/crid/1370850475165518209","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Increased PADI4 expression in blood and tissue of patients with malignant tumors"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204901072896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"<b>Protein Deimination in the Rat Brain: Generation of Citrulline-Containing 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