Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter
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説明
The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins.
収録刊行物
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- Bioorganic & Medicinal Chemistry Letters
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Bioorganic & Medicinal Chemistry Letters 22 (2), 1251-1254, 2012-01
Elsevier BV