{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360567182219633024.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.jdermsci.2015.05.008"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0923181115300013?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0923181115300013?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"26126707"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Rotation is the primary motion of paired human epidermal keratinocytes"}],"description":[{"notation":[{"@value":"Collective motion of keratinocytes is involved in morphogenesis, homeostasis, and wound healing of the epidermis. Yet how the collective motion of keratinocytes emerges from the behavior of individual cells is still largely unknown.The aim of this study was to find the cellular behavior that links single and collective motion of keratinocytes.We investigated the behavior of two-cell colonies of HaCaT keratinocytes by a combination of time-lapse imaging and image processing.The two-cell colonies of HaCaT cells were formed as a contacted pair of keratinocyte clones. Image analysis and cell culture experiments revealed that the rotational speed of two-cell colonies was positively associated with their proliferative capacity. α6 integrin was required for the rotational motion of two-cell keratinocyte colonies. We also confirmed that two-cell colonies of keratinocytes predominantly exhibited the rotational, but not translational, motion, two modes of motion in a contact pair of rotating objects.The rotational motion is the primary motion of two-cell keratinocyte colonies and its speed is positively associated with their proliferative capacity. This study suggests that the assembly of rotating keratinocytes generates the collective motion of proliferative keratinocytes during morphogenesis and wound healing of the epidermis."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380567182219633026","@type":"Researcher","foaf:name":[{"@value":"Sota Tate"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567182219633027","@type":"Researcher","foaf:name":[{"@value":"Matome Imai"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567182219633029","@type":"Researcher","foaf:name":[{"@value":"Natsuki Matsushita"}]},{"@id":"https://cir.nii.ac.jp/crid/1380567182219633028","@type":"Researcher","foaf:name":[{"@value":"Emi K. Nishimura"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751163651584","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60202272"},{"@type":"NRID","@value":"1000060202272"},{"@type":"NRID","@value":"9000007593675"},{"@type":"NRID","@value":"9000016487806"},{"@type":"NRID","@value":"9000253176508"},{"@type":"NRID","@value":"9000255960035"},{"@type":"NRID","@value":"9000001114780"},{"@type":"NRID","@value":"9000010428474"},{"@type":"NRID","@value":"9000014673783"},{"@type":"NRID","@value":"9000015325794"},{"@type":"NRID","@value":"9000399157999"},{"@type":"NRID","@value":"9000347091936"},{"@type":"NRID","@value":"9000004088666"},{"@type":"NRID","@value":"9000003874571"},{"@type":"NRID","@value":"9000253181590"},{"@type":"NRID","@value":"9000004033801"},{"@type":"NRID","@value":"9000252858883"},{"@type":"NRID","@value":"9000376945781"},{"@type":"NRID","@value":"9000005711358"},{"@type":"NRID","@value":"9000391584279"},{"@type":"NRID","@value":"9000008798291"},{"@type":"NRID","@value":"9000001264424"},{"@type":"NRID","@value":"9000019129986"},{"@type":"NRID","@value":"9000238283283"},{"@type":"NRID","@value":"9000397851078"},{"@type":"NRID","@value":"9000253179611"},{"@type":"NRID","@value":"9000252814336"},{"@type":"NRID","@value":"9000253176388"},{"@type":"NRID","@value":"9000375911893"}],"foaf:name":[{"@value":"Shigeki Higashiyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751158428672","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10380255"},{"@type":"NRID","@value":"1000010380255"},{"@type":"NRID","@value":"9000255960036"},{"@type":"NRID","@value":"9000016089431"},{"@type":"NRID","@value":"9000019110442"},{"@type":"NRID","@value":"9000238936716"},{"@type":"NRID","@value":"9000361227241"},{"@type":"NRID","@value":"9000001692734"},{"@type":"NRID","@value":"9000375849596"},{"@type":"NRID","@value":"9000019140155"},{"@type":"NRID","@value":"9000241782426"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0152625"}],"foaf:name":[{"@value":"Daisuke Nanba"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09231811"}],"prism:publicationName":[{"@value":"Journal of Dermatological Science"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2015-09","prism:volume":"79","prism:number":"3","prism:startingPage":"194","prism:endingPage":"202"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0923181115300013?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0923181115300013?httpAccept=text/plain"}],"createdAt":"2015-06-13","modifiedAt":"2022-05-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Keratinocytes","dc:title":"Keratinocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Rotation","dc:title":"Rotation"},{"@id":"https://cir.nii.ac.jp/all?q=Integrin%20alpha6","dc:title":"Integrin alpha6"},{"@id":"https://cir.nii.ac.jp/all?q=Time-Lapse%20Imaging","dc:title":"Time-Lapse Imaging"},{"@id":"https://cir.nii.ac.jp/all?q=Coculture%20Techniques","dc:title":"Coculture Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Clone%20Cells","dc:title":"Clone Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Movement","dc:title":"Cell Movement"},{"@id":"https://cir.nii.ac.jp/all?q=Image%20Processing,%20Computer-Assisted","dc:title":"Image Processing, Computer-Assisted"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Proliferation","dc:title":"Cell Proliferation"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257280856832","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26461660"},{"@type":"JGN","@value":"JP26461660"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26461660/"}],"notation":[{"@language":"ja","@value":"ヒト表皮多層化に伴う細胞動態とアクチン繊維動態の全系譜解析"},{"@language":"en","@value":"Lineage analysis of human epidermal keratinocytes and their actin filaments during 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