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The Cellular Factors Vps18 and Mon2 Are Required for Efficient Production of Infectious HIV-1 Particles
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- Yuriko Tomita
- Division of Virology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
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- Takeshi Noda
- International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
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- Ken Fujii
- Division of Virology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
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- Tokiko Watanabe
- ERATO Infection-Induced Host Responses Project, Saitama 332-0012, Japan
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- Yuko Morikawa
- Kitasato Institute for Life Science and Graduate School for Infection Control, Kitasato University, Shirokane 5-9-1, Minato-ku, Tokyo 108-8641, Japan
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- Yoshihiro Kawaoka
- Division of Virology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
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Description
<jats:title>ABSTRACT</jats:title> <jats:p> Like all viruses, HIV-1 requires cellular host factors for replication. The mechanisms for production of progeny virions involving these host factors, however, are not fully understood. To better understand these mechanisms, we used a yeast ( <jats:named-content content-type="genus-species">Saccharomyces cerevisiae</jats:named-content> ) genetic screen to identify mutant strains in which HIV-1 Gag targeting to the plasma membrane was aberrant. Of the 917 mutants identified, we selected 14 mutants whose missing genes had single orthologous counterparts in human and tested them for Gag-induced viruslike particle (VLP) release in yeast cells. We found that the Vps18 and Mon2 proteins were important for HIV-1 Gag-induced VLP release in yeast. In eukaryote cells, these host proteins are highly conserved and function in protein trafficking. Depletion of hVps18 or hMon2 reduced the efficient production of infectious HIV-1 virions in human cells. Our data suggest that these cellular factors play an important role in the efficient production of infectious HIV-1 virion particles. </jats:p>
Journal
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- Journal of Virology
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Journal of Virology 85 (11), 5618-5627, 2011-06
American Society for Microbiology
