- Integration of CiNii Books functions for fiscal year 2025 has completed
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on November 26, 2025】Regarding the recording of “Research Data” and “Evidence Data”
- Incorporated Jxiv preprints from JaLC and adding coverage from NDL Search
Connect-seq to superimpose molecular on anatomical neural circuit maps
-
- Naresh K. Hanchate
- Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
-
- Donghui Kuang
- Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
-
- Eun Jeong Lee
- Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
-
- Ryan Basom
- Genomics and Bioinformatics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
-
- Cole Trapnell
- Department of Genome Sciences, University of Washington, Seattle, WA 98115;
-
- Kunio Kondoh
- Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
-
- Andria Ellis
- Department of Genome Sciences, University of Washington, Seattle, WA 98115;
-
- Linda B. Buck
- Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
Bibliographic Information
- Published
- 2020-02-07
- Resource Type
- journal article
- Rights Information
-
- https://www.pnas.org/site/aboutpnas/licenses.xhtml
- DOI
-
- 10.1073/pnas.1912176117
- 10.1101/454835
- Publisher
- Proceedings of the National Academy of Sciences
Search this article
Description
<jats:p>The mouse brain contains about 75 million neurons interconnected in a vast array of neural circuits. The identities and functions of individual neuronal components of most circuits are undefined. Here we describe a method, termed “Connect-seq,” which combines retrograde viral tracing and single-cell transcriptomics to uncover the molecular identities of upstream neurons in a specific circuit and the signaling molecules they use to communicate. Connect-seq can generate a molecular map that can be superimposed on a neuroanatomical map to permit molecular and genetic interrogation of how the neuronal components of a circuit control its function. Application of this method to hypothalamic neurons controlling physiological responses to fear and stress reveals subsets of upstream neurons that express diverse constellations of signaling molecules and can be distinguished by their anatomical locations.</jats:p>
Journal
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 117 (8), 4375-4384, 2020-02-07
Proceedings of the National Academy of Sciences
