Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals
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- Mervin C. Yoder
- Department of Pediatrics,
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- Laura E. Mead
- Department of Pediatrics,
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- Daniel Prater
- Department of Pediatrics,
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- Theresa R. Krier
- Department of Pediatrics,
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- Karim N. Mroueh
- Department of Pediatrics,
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- Fang Li
- Department of Pediatrics,
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- Rachel Krasich
- Department of Pediatrics,
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- Constance J. Temm
- Department of Pediatrics,
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- Josef T. Prchal
- Division of Hematology/Oncology, Center for Cell and Gene Therapy, and Hematology, University of Utah, Salt Lake City
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- David A. Ingram
- Department of Pediatrics,
説明
<jats:title>Abstract</jats:title><jats:p>The limited vessel-forming capacity of infused endothelial progenitor cells (EPCs) into patients with cardiovascular dysfunction may be related to a misunderstanding of the biologic potential of the cells. EPCs are generally identified by cell surface antigen expression or counting in a commercially available kit that identifies “endothelial cell colony-forming units” (CFU-ECs). However, the origin, proliferative potential, and differentiation capacity of CFU-ECs is controversial. In contrast, other EPCs with blood vessel-forming ability, termed endothelial colony-forming cells (ECFCs), have been isolated from human peripheral blood. We compared the function of CFU-ECs and ECFCs and determined that CFU-ECs are derived from the hematopoietic system using progenitor assays, and analysis of donor cells from polycythemia vera patients harboring a Janus kinase 2 V617F mutation in hematopoietic stem cell clones. Further, CFU-ECs possess myeloid progenitor cell activity, differentiate into phagocytic macrophages, and fail to form perfused vessels in vivo. In contrast, ECFCs are clonally distinct from CFU-ECs, display robust proliferative potential, and form perfused vessels in vivo. Thus, these studies establish that CFU-ECs are not EPCs and the role of these cells in angiogenesis must be re-examined prior to further clinical trials, whereas ECFCs may serve as a potential therapy for vascular regeneration.</jats:p>
収録刊行物
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- Blood
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Blood 109 (5), 1801-1809, 2006-10-19
American Society of Hematology