{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360574093767489792.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1365-2486.2005.01076.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2486.2005.01076.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2486.2005.01076.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1365-2486.2005.01076.x"}}],"dc:title":[{"@value":"Variation of carbon isotope fractionation in hydrogenotrophic methanogenic microbial cultures and environmental samples at different energy status"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Methane is a major product of anaerobic degradation of organic matter and an important greenhouse gas. Its stable carbon isotope composition can be used to reveal active methanogenic pathways, if associated isotope fractionation factors are known. To clarify the causes that lead to the wide variation of fractionation factors of methanogenesis from H<jats:sub>2</jats:sub> plus CO<jats:sub>2</jats:sub> (<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/GCB_1076_mu4.gif\" xlink:title=\"inline image\"/>), pure cultures and various cocultures were grown under different thermodynamic conditions. In syntrophic and obligate syntrophic cocultures thriving on different carbohydrate substrates, fermentative bacteria were coupled to three different species of hydrogenotrophic methanogens of the families Methanobacteriaceae and Methanomicrobiaceae. We found that C‐isotope fractionation was correlated to the Gibbs free energy change (Δ<jats:italic>G</jats:italic>) of CH<jats:sub>4</jats:sub> formation from H<jats:sub>2</jats:sub> plus CO<jats:sub>2</jats:sub> and that the relation can be described by a semi‐Gauss curve. The derived relationship was used to quantify the average Δ<jats:italic>G</jats:italic> that is available to hydrogenotrophic methanogenic archaea in their habitat, thus avoiding the problems encountered with measurement of low H<jats:sub>2</jats:sub> concentrations on a microscale. Boreal peat, rice field soil, and rumen fluid, which represent major sources of atmospheric CH<jats:sub>4</jats:sub>, exhibited increasingly smaller <jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/GCB_1076_mu5.gif\" xlink:title=\"inline image\"/>, indicating that thermodynamic conditions for hydrogenotrophic methanogens became increasingly more favourable. Vice versa, we hypothesize that environments with similar energetic conditions will also exhibit similar isotope fractionation. Our results, thus, provide a mechanistic constraint for modelling the <jats:sup>13</jats:sup>C flux from microbial sources of atmospheric CH<jats:sub>4</jats:sub>.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380574093767489794","@type":"Researcher","foaf:name":[{"@value":"HOLGER PENNING"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574093767489664","@type":"Researcher","foaf:name":[{"@value":"CAROLINE M. PLUGGE"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574093767489792","@type":"Researcher","foaf:name":[{"@value":"PIERRE E. GALAND"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574093767489793","@type":"Researcher","foaf:name":[{"@value":"RALF CONRAD"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13541013"},{"@type":"EISSN","@value":"13652486"}],"prism:publicationName":[{"@value":"Global Change Biology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2005-11-28","prism:volume":"11","prism:number":"12","prism:startingPage":"2103","prism:endingPage":"2113"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2486.2005.01076.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2486.2005.01076.x"},{"@id":"https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1365-2486.2005.01076.x"}],"createdAt":"2005-11-21","modifiedAt":"2023-08-28","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215857603456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Geochemical origin of hydrothermal fluid methane in sediment-associated fields and its relevance to the geographical distribution of whole hydrothermal 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on the Nankai Accretionary Prism"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204553019904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hydrothermal fluid geochemistry at the Iheya North field in the mid-Okinawa Trough: Implication for origin of methane in subseafloor fluid circulation systems"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001288156091520","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Compositional and isotopic characteristics of hydrocarbons generated by a hydrothermal experiment simulating seafloor sediment alteration stepwise heating from 275 to 361°C at 30 MPa"}]},{"@id":"https://cir.nii.ac.jp/crid/1390003825207963392","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Microbial methane production and oxidation in the 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isotope systematics in hydrogenotrophic methanogenesis under H2-limited and H2-enriched conditions : implications for the origin of methane and its isotopic 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