{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360574094666164352.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/rcm.5298"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.5298"}},{"identifier":{"@type":"URI","@value":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.5298"}}],"dc:title":[{"@value":"Using gas chromatography/isotope ratio mass spectrometry to determine the fractionation factor for H\n                    <sub>2</sub>\n                    production by hydrogenases"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                    Hydrogenases catalyze the reversible formation of H\n                    <jats:sub>2</jats:sub>\n                    , and they are key enzymes in the biological cycling of H\n                    <jats:sub>2</jats:sub>\n                    . H isotopes have the potential to be a very useful tool in quantifying hydrogen ion trafficking in biological H\n                    <jats:sub>2</jats:sub>\n                    production processes, but there are several obstacles that have thus far limited the application of this tool. Here, we describe a new method that overcomes some of these barriers and is specifically designed to measure isotopic fractionation during enzyme‐catalyzed H\n                    <jats:sub>2</jats:sub>\n                    evolution. A key feature of this technique is that purified hydrogenases are employed, allowing precise control over the reaction conditions and therefore a high level of precision. In addition, a custom‐designed high‐throughput gas chromatograph/isotope ratio mass spectrometer is employed to measure the isotope ratio of the H\n                    <jats:sub>2</jats:sub>\n                    . Using our new approach, we determined that the fractionation factor for H\n                    <jats:sub>2</jats:sub>\n                    production by the [NiFe]‐hydrogenase from\n                    <jats:italic>Desulfovibrio fructosovorans</jats:italic>\n                    is 0.273 ± 0.006. This result indicates that, as expected, protons are highly favored over deuterium ions during H\n                    <jats:sub>2</jats:sub>\n                    evolution. Potential applications of this newly developed method are discussed. Copyright © 2011 John Wiley & Sons, Ltd.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380574094666164354","@type":"Researcher","foaf:name":[{"@value":"Hui Yang"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry & Molecular Biology Michigan State University  East Lansing MI 48824‐1319 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574094666164353","@type":"Researcher","foaf:name":[{"@value":"Hasand Gandhi"}],"jpcoar:affiliationName":[{"@value":"Department of Zoology Michigan State University  East Lansing MI 48824 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574094666164357","@type":"Researcher","foaf:name":[{"@value":"Liang Shi"}],"jpcoar:affiliationName":[{"@value":"Chemical and Biological Sciences Division, Pacific Northwest National Laboratory  Richland WA 99350 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574094666164352","@type":"Researcher","foaf:name":[{"@value":"Helen W. Kreuzer"}],"jpcoar:affiliationName":[{"@value":"Chemical and Biological Sciences Division, Pacific Northwest National Laboratory  Richland WA 99350 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574094666164356","@type":"Researcher","foaf:name":[{"@value":"Nathaniel E. Ostrom"}],"jpcoar:affiliationName":[{"@value":"Department of Zoology Michigan State University  East Lansing MI 48824 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380574094666164355","@type":"Researcher","foaf:name":[{"@value":"Eric L. Hegg"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry & Molecular Biology Michigan State University  East Lansing MI 48824‐1319 USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09514198"},{"@type":"EISSN","@value":"10970231"}],"prism:publicationName":[{"@value":"Rapid Communications in Mass Spectrometry"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2011-12-02","prism:volume":"26","prism:number":"1","prism:startingPage":"61","prism:endingPage":"68"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.5298"},{"@id":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.5298"}],"createdAt":"2011-12-09","modifiedAt":"2025-10-26","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232166617728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Hydrogen isotope systematics among H2–H2O–CH4 during the growth of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus strain ΔH"}]},{"@id":"https://cir.nii.ac.jp/crid/2050307416977929728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Hydrogen and carbon isotope systematics in hydrogenotrophic methanogenesis under H2-limited and H2-enriched conditions : implications for the origin of methane and its isotopic diagnosis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/rcm.5298"},{"@type":"CROSSREF","@value":"10.1016/j.gca.2014.07.020_references_DOI_EiYkW246SGp1CZkl9Clc7TGcZO"},{"@type":"CROSSREF","@value":"10.1186/s40645-016-0088-3_references_DOI_EiYkW246SGp1CZkl9Clc7TGcZO"}]}