Pattern Recognition of microRNA Expression in Body Fluids Using Nanopore Decoding at Subfemtomolar Concentrations
-
- Nanami Takeuchi
- Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
-
- Moe Hiratani
- Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
-
- Ryuji Kawano
- Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
書誌事項
- 公開日
- 2022-06-26
- 資源種別
- journal article
- 権利情報
-
- https://creativecommons.org/licenses/by-nc-nd/4.0/
- DOI
-
- 10.1021/jacsau.2c00117
- 10.26434/chemrxiv-2021-p71pl-v2
- 10.26434/chemrxiv-2021-p71pl
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
<jats:p>This paper describes nanopore decoding for microRNA (miRNA) expression patterns using DNA computing technology. miRNAs have shown promise as markers for cancer diagnosis due to their cancer type-specificity, and therefore simple strategies for miRNA-pattern recognition are required. We propose a system for pattern recognition of five types of miRNAs overexpressed in bile duct cancer (BDC). The information of miRNAs from BDC is encoded in diagnostic DNAs (dgDNAs) and decoded electrically by nanopore measurement. With this system, we succeeded in distinguishing miRNA expression patterns in the plasma of BDC patients using a label-free method and in real-time. Moreover, our dgDNA-miRNAs complexes can be captured by the nanopore at ultralow concentration, such as 0.1 fM. Such nanopore decoding with dgDNAs could be applied as a simple and early diagnostic tool for cancer in the future.</jats:p>
収録刊行物
-
- JACS Au
-
JACS Au 2 (8), 1829-1838, 2022-06-26
American Chemical Society (ACS)
