{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360580240134248832.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1242/jcs.240713"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1242/jcs.240713"}},{"identifier":{"@type":"URI","@value":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.240713/3509892/jcs240713.pdf"}}],"dc:title":[{"@value":"The cell biologist's guide to super-resolution microscopy"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n                  <jats:p>Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, the resolution of light microscopy is limited by the laws of diffraction to a few hundred nanometers, blurring most cellular details. Over the last two decades, several techniques – grouped under the ‘super-resolution microscopy’ moniker – have been designed to bypass this limitation, revealing the cellular organization down to the nanoscale. The number and variety of these techniques have steadily increased, to the point that it has become difficult for cell biologists and seasoned microscopists alike to identify the specific technique best suited to their needs. Available techniques include image processing strategies that generate super-resolved images, optical imaging schemes that overcome the diffraction limit and sample manipulations that expand the size of the biological sample. In this Cell Science at a Glance article and the accompanying poster, we provide key pointers to help users navigate through the various super-resolution methods by briefly summarizing the principles behind each technique, highlighting both critical strengths and weaknesses, as well as providing example images.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380580240134248834","@type":"Researcher","foaf:name":[{"@value":"Guillaume Jacquemet"}],"jpcoar:affiliationName":[{"@value":"Turku 1   , FI-20520 Turku , Finland"},{"@value":"Bioscience Centre, University of Turku and Åbo Akademi University 1   , FI-20520 Turku , Finland"},{"@value":"Åbo Akademi University 2 Faculty of Science and Engineering, Cell Biology , , 20520 Turku , Finland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380580240134248833","@type":"Researcher","foaf:name":[{"@value":"Alexandre F. Carisey"}],"jpcoar:affiliationName":[{"@value":"William T. Shearer Center for Human Immunobiology, Baylor College of Medicine and Texas Children's Hospital 3 , 1102 Bates Street, Houston 77030 TX , USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380580240134248835","@type":"Researcher","foaf:name":[{"@value":"Hellyeh Hamidi"}],"jpcoar:affiliationName":[{"@value":"Turku 1   , FI-20520 Turku , Finland"},{"@value":"Bioscience Centre, University of Turku and Åbo Akademi University 1   , FI-20520 Turku , Finland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380580240134248832","@type":"Researcher","foaf:name":[{"@value":"Ricardo Henriques"}],"jpcoar:affiliationName":[{"@value":"University College London 4 , London WC1E 6BT , UK"},{"@value":"The Francis Crick Institute 5 , London NW1 1AT , UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1380580240134248836","@type":"Researcher","foaf:name":[{"@value":"Christophe Leterrier"}],"jpcoar:affiliationName":[{"@value":"Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto 6 , Marseille 13015 , France"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14779137"},{"@type":"PISSN","@value":"00219533"}],"prism:publicationName":[{"@value":"Journal of Cell Science"}],"dc:publisher":[{"@value":"The Company of Biologists"}],"prism:publicationDate":"2020-06-01","prism:volume":"133","prism:number":"11","prism:startingPage":"jcs240713"},"reviewed":"false","dc:rights":["http://www.biologists.com/user-licence-1-1"],"url":[{"@id":"https://syndication.highwire.org/content/doi/10.1242/jcs.240713"},{"@id":"https://journals.biologists.com/jcs/article-pdf/doi/10.1242/jcs.240713/3509892/jcs240713.pdf"}],"createdAt":"2020-06-11","modifiedAt":"2024-06-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360298757449326208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The 2022 applied physics by pioneering women: a roadmap"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865814751575296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Getting sharper: the brain under the spotlight of super-resolution microscopy"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1242/jcs.240713"},{"@type":"CROSSREF","@value":"10.1088/1361-6463/ac82f9_references_DOI_Z1prBQCRyEcaPbX4kWUwXliNYHV"},{"@type":"CROSSREF","@value":"10.1016/j.tcb.2022.06.011_references_DOI_Z1prBQCRyEcaPbX4kWUwXliNYHV"}]}