{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360846642777067904.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1104/pp.15.00319"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1104/pp.15.00319"}},{"identifier":{"@type":"PMID","@value":"25922058"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Algal Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase, Triacylglycerol Accumulation Regulator1, Regulates Accumulation of Triacylglycerol in Nitrogen or Sulfur Deficiency"}],"description":[{"notation":[{"@value":"Although microalgae accumulate triacylglycerol (TAG) and starch in response to nutrient-deficient conditions, the regulatory mechanisms are poorly understood. We report here the identification and characterization of a kinase, triacylglycerol accumulation regulator1 (TAR1), that is a member of the yeast (Saccharomyces cerevisiae) Yet another kinase1 (Yak1) subfamily in the dual-specificity tyrosine phosphorylation-regulated kinase family in a green alga (Chlamydomonas reinhardtii). The kinase domain of TAR1 showed auto- and transphosphorylation activities. A TAR1-defective mutant, tar1-1, accumulated TAG to levels 0.5- and 0.1-fold of those of a wild-type strain in sulfur (S)- and nitrogen (N)-deficient conditions, respectively. In N-deficient conditions, tar1-1 showed more pronounced arrest of cell division than the wild type, had increased cell size and cell dry weight, and maintained chlorophyll and photosynthetic activity, which were not observed in S-deficient conditions. In N-deficient conditions, global changes in expression levels of N deficiency-responsive genes in N assimilation and tetrapyrrole metabolism were noted between tar1-1 and wild-type cells. These results indicated that TAR1 is a regulator of TAG accumulation in S- and N-deficient conditions, and it functions in cell growth and repression of photosynthesis in conditions of N deficiency."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420564276163175680","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40594437"},{"@type":"NRID","@value":"1000040594437"},{"@type":"ORCID","@value":"0000-0003-0361-2769"},{"@type":"NRID","@value":"9000004258577"},{"@type":"NRID","@value":"9000336038363"},{"@type":"NRID","@value":"9000017582724"},{"@type":"NRID","@value":"9000391944802"},{"@type":"NRID","@value":"9000414262678"},{"@type":"NRID","@value":"9000391934829"},{"@type":"NRID","@value":"9000391947443"},{"@type":"NRID","@value":"9000391956604"},{"@type":"NRID","@value":"9000391955010"},{"@type":"NRID","@value":"9000019089878"},{"@type":"NRID","@value":"9000242546968"},{"@type":"NRID","@value":"9000331453439"},{"@type":"NRID","@value":"9000242251384"},{"@type":"NRID","@value":"9000019194273"},{"@type":"NRID","@value":"9000259864109"},{"@type":"NRID","@value":"9000413745105"},{"@type":"NRID","@value":"9000391940558"},{"@type":"NRID","@value":"9000242251367"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/masataka.kajikawa"}],"foaf:name":[{"@value":"Masataka Kajikawa"}],"jpcoar:affiliationName":[{"@value":"Graduate School of Biostudies, Kyoto University, Kyoto 606–8502, Japan (Ma.K., Y.S., H.S., T.Y., H.F.);"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358279","@type":"Researcher","foaf:name":[{"@value":"Yuri Sawaragi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358274","@type":"Researcher","foaf:name":[{"@value":"Haruka Shinkawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358272","@type":"Researcher","foaf:name":[{"@value":"Takashi Yamano"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358273","@type":"Researcher","foaf:name":[{"@value":"Akira Ando"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358278","@type":"Researcher","foaf:name":[{"@value":"Misako Kato"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276159206272","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10212177"},{"@type":"NRID","@value":"1000010212177"},{"@type":"NRID","@value":"9000002738331"},{"@type":"NRID","@value":"9000253108457"},{"@type":"NRID","@value":"9000000028936"},{"@type":"NRID","@value":"9000004916967"},{"@type":"NRID","@value":"9000253108098"},{"@type":"NRID","@value":"9000007986390"},{"@type":"NRID","@value":"9000391629050"},{"@type":"NRID","@value":"9000391609591"},{"@type":"NRID","@value":"9000008109237"},{"@type":"NRID","@value":"9000242546969"},{"@type":"NRID","@value":"9000391611186"},{"@type":"NRID","@value":"9000397692348"},{"@type":"NRID","@value":"9000253166512"},{"@type":"NRID","@value":"9000252810758"},{"@type":"NRID","@value":"9000253169603"},{"@type":"NRID","@value":"9000009033593"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0180653"}],"foaf:name":[{"@value":"Masafumi Hirono"}]},{"@id":"https://cir.nii.ac.jp/crid/1380847870996358275","@type":"Researcher","foaf:name":[{"@value":"Naoki Sato"}]},{"@id":"https://cir.nii.ac.jp/crid/1380846642777067904","@type":"Researcher","foaf:name":[{"@value":"Hideya Fukuzawa"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00320889"},{"@type":"EISSN","@value":"15322548"}],"prism:publicationName":[{"@value":"Plant Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2015-04-28","prism:volume":"168","prism:number":"2","prism:startingPage":"752","prism:endingPage":"764"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://aspb.org/publications/aspb-journals/open-articles"],"url":[{"@id":"https://syndication.highwire.org/content/doi/10.1104/pp.15.00319"}],"createdAt":"2015-06-01","modifiedAt":"2021-02-17","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Nitrogen","dc:title":"Nitrogen"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Profiling","dc:title":"Gene Expression Profiling"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Starch","dc:title":"Starch"},{"@id":"https://cir.nii.ac.jp/all?q=Lipid%20Droplets","dc:title":"Lipid Droplets"},{"@id":"https://cir.nii.ac.jp/all?q=Flow%20Cytometry","dc:title":"Flow Cytometry"},{"@id":"https://cir.nii.ac.jp/all?q=Genes,%20Plant","dc:title":"Genes, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Models,%20Biological","dc:title":"Models, Biological"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Structure,%20Tertiary","dc:title":"Protein Structure, Tertiary"},{"@id":"https://cir.nii.ac.jp/all?q=Phenotype","dc:title":"Phenotype"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Plant","dc:title":"Gene Expression Regulation, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Tyrosine","dc:title":"Tyrosine"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphorylation","dc:title":"Phosphorylation"},{"@id":"https://cir.nii.ac.jp/all?q=Chlamydomonas%20reinhardtii","dc:title":"Chlamydomonas reinhardtii"},{"@id":"https://cir.nii.ac.jp/all?q=Phylogeny","dc:title":"Phylogeny"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfur","dc:title":"Sulfur"},{"@id":"https://cir.nii.ac.jp/all?q=Triglycerides","dc:title":"Triglycerides"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Proteins","dc:title":"Plant Proteins"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782199242624","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"24370079"},{"@type":"JGN","@value":"JP24370079"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24370079/"}],"notation":[{"@language":"ja","@value":"中心子の９回対称性構造構築におけるカートホイール非依存的機構の解明"},{"@language":"en","@value":"Elusidation of cartwheel-independent mechanisms for establishing 9-fold symmetry of the cdentriole 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