- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Genome of alkaliphilic <i>Bacillus pseudofirmus</i> OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4
Search this article
Description
<jats:title>Summary</jats:title><jats:p><jats:italic>Bacillus pseudofirmus</jats:italic> OF4 is an extreme but facultative alkaliphile that grows non‐fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH > 9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of <jats:italic>B. pseudofirmus</jats:italic> OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.</jats:p>
Journal
-
- Environmental Microbiology
-
Environmental Microbiology 13 (12), 3289-3309, 2011-09-27
Wiley
