{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360848655415796992.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/978-1-4939-8879-2_11"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/978-1-4939-8879-2_11"}},{"identifier":{"@type":"PMID","@value":"30378049"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding"}],"description":[{"notation":[{"@value":"Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380848655415796868","@type":"Researcher","foaf:name":[{"@value":"Takashi Okumura"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848655415796864","@type":"Researcher","foaf:name":[{"@value":"Kenoki Ohuchida"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848655415797001","@type":"Researcher","foaf:name":[{"@value":"Masafumi Nakamura"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10643745"},{"@type":"EISSN","@value":"19406029"},{"@type":"ISBN","@value":"9781493988785"},{"@type":"ISBN","@value":"9781493988792"}],"prism:publicationName":[{"@value":"Methods in Molecular Biology"}],"dc:publisher":[{"@value":"Springer New York"}],"prism:publicationDate":"2018-10-31","prism:startingPage":"135","prism:endingPage":"141"},"reviewed":"false","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/978-1-4939-8879-2_11"}],"createdAt":"2018-10-30","modifiedAt":"2018-10-30","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Culture%20Techniques","dc:title":"Cell Culture Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Intra-Abdominal%20Fat","dc:title":"Intra-Abdominal Fat"},{"@id":"https://cir.nii.ac.jp/all?q=Collagen%20Type%20I","dc:title":"Collagen Type I"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Pancreatic%20Neoplasms","dc:title":"Pancreatic Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Adipocytes","dc:title":"Adipocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Tumor%20Cells,%20Cultured","dc:title":"Tumor Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Neoplasm%20Invasiveness","dc:title":"Neoplasm Invasiveness"},{"@id":"https://cir.nii.ac.jp/all?q=Stromal%20Cells","dc:title":"Stromal Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Gels","dc:title":"Gels"},{"@id":"https://cir.nii.ac.jp/all?q=Pancreas","dc:title":"Pancreas"},{"@id":"https://cir.nii.ac.jp/all?q=Carcinoma,%20Pancreatic%20Ductal","dc:title":"Carcinoma, Pancreatic Ductal"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781902487168","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16H06258"},{"@type":"JGN","@value":"JP16H06258"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H06258/"}],"notation":[{"@language":"ja","@value":"オルガノイドを用いた膵癌phenotypeによる膵星細胞の基質リモデリング解析"},{"@language":"en","@value":"Elucidation of the difference in extracellular matrix remodeling ability of pancreatic stellate cells depending on pancreatic cancer phenotype via organoid models"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000781957670400","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"17H04284"},{"@type":"JGN","@value":"JP17H04284"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04284/"}],"notation":[{"@language":"ja","@value":"膵オルガノイドを用いたリーディングPSC誘導基底膜破壊機序解明と基底膜制御法開発"},{"@language":"en","@value":"Elucidation of leading PSC induced basement membrane destruction mechanism and development of the regulating method by using pancreatic organoids"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000781975323904","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"17K19602"},{"@type":"JGN","@value":"JP17K19602"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K19602/"}],"notation":[{"@language":"ja","@value":"膵癌進展・防御に関わる微小環境のphenotype 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