{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360848656925101056.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.bbagen.2014.04.014"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0304416514001470?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0304416514001470?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"24769178"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"N-acetyl-l-methionine is a superior protectant of human serum albumin against photo-oxidation and reactive oxygen species compared to N-acetyl-l-tryptophan"}],"description":[{"notation":[{"@value":"Sodium octanoate (Oct) and N-acetyl-l-tryptophan (N-AcTrp) are widely used as stabilizers during pasteurization and storage of albumin products. However, exposure to light photo-degrades N-AcTrp with the formation of potentially toxic compounds. Therefore, we have examined the usefulness of N-acetyl-l-methionine (N-AcMet) in comparison with N-AcTrp for long-term stability, including photo stability, of albumin products.Recombinant human serum albumin (rHSA) with and without additives was photo-irradiated for 4weeks. The capability of the different stabilizers to scavenge reactive oxygen species (ROS) was examined by ESR spectrometry. Carbonyl contents were assessed by a spectrophotometric method using fluoresceinamine and Western blotting, whereas the structure of rHSA was examined by SDS-PAGE, far-UV circular dichroism and differential scanning calorimetry. Binding was determined by ultrafiltration.N-AcMet was found to be a superior ROS scavenger both before and after photo-irradiation. The number of carbonyl groups formed was lowest in the presence of N-AcMet. According to SDS-PAGE, N-AcMet stabilizes the monomeric form of rHSA, whereas N-AcTrp induces degradation of rHSA during photo-irradiation. The decrease in α-helical content of rHSA was the smallest in the presence of Oct, without or with N-AcMet. Photo-irradiation did not affect the denaturation temperature or calorimetric enthalpy of rHSA, when N-AcMet was present.The weakly bound N-AcMet is a superior protectant of albumin, because it is a better ROS-protector and structural stabilizer than N-AcTrp, and it is probable and also useful for other protein preparations.N-AcMet is an effective stabilizer of albumin during photo-irradiation, while N-Ac-Trp promotes photo-oxidative damage to albumin."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380848656925101059","@type":"Researcher","foaf:name":[{"@value":"Yousuke 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Otagiri"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03044165"}],"prism:publicationName":[{"@value":"Biochimica et Biophysica Acta (BBA) - General Subjects"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2014-09","prism:volume":"1840","prism:number":"9","prism:startingPage":"2806","prism:endingPage":"2812"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0304416514001470?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0304416514001470?httpAccept=text/plain"}],"createdAt":"2014-04-22","modifiedAt":"2022-04-02","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Stability","dc:title":"Protein Stability"},{"@id":"https://cir.nii.ac.jp/all?q=Tryptophan","dc:title":"Tryptophan"},{"@id":"https://cir.nii.ac.jp/all?q=Free%20Radical%20Scavengers","dc:title":"Free Radical Scavengers"},{"@id":"https://cir.nii.ac.jp/all?q=Photochemical%20Processes","dc:title":"Photochemical Processes"},{"@id":"https://cir.nii.ac.jp/all?q=Methionine","dc:title":"Methionine"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Reactive%20Oxygen%20Species","dc:title":"Reactive Oxygen Species"},{"@id":"https://cir.nii.ac.jp/all?q=Oxidation-Reduction","dc:title":"Oxidation-Reduction"},{"@id":"https://cir.nii.ac.jp/all?q=Serum%20Albumin","dc:title":"Serum Albumin"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782184873472","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23790211"},{"@type":"JGN","@value":"JP23790211"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790211/"}],"notation":[{"@language":"ja","@value":"生活習慣病に対する治療薬剤及び補完代替物の多面的抗酸化能評価"},{"@language":"en","@value":"Multifaceted antioxidant evaluation of medicine and functional food for the lifestyle-related 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