{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360848657196288256.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.jdermsci.2013.10.008"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0923181113003538?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0923181113003538?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"24252747"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Neuronatin is related to keratinocyte differentiation by up-regulating involucrin"}],"description":[{"notation":[{"@value":"Neuronatin (Nnat), which is a neuronal developmental and differentiation molecule, is expressed in the endoplasmic reticulum of non-neuronal cells and is involved in insulin secretion from pancreatic β-cells by plausibly modulating their intracellular calcium concentration. However, the role of Nnat in keratinocyte differentiation remains unclear.To unveil a possible integration of Nnat in controlling the keratinocyte differentiation markers such as involucrin, cytokeratin1, filaggrin, loricrin and S100A7.Immunohistological staining was done using psoriasis, chronic eczema, lichen planus and normal skin. Immunofluorescence staining, Western blotting and semi-quantitative real-time PCR were performed for detecting Nnat, involucrin, cytokeratin1, filaggrin, loricrin and S100A7 using human keratinocytes with or without Nnat gene transfection. Small interference RNA was applied to knockdown the Nnat gene expression.Nnat existed in normal human epidermis and cultured keratinocytes. In the hyperplastic epidermis of psoriasis, chronic eczema and lichen planus, over-expression of Nnat was evident along with involucrin and cytokeratin1 expression. Coordinate up-regulation of Nnat and involucrin, but not cytokeratin1, was demonstrated in cultured keratinocytes under differentiation stimuli such as extracellular calcium elevation, exposure to phorbol myristate acetate, and increased cell density. Transfection of small intereference RNA for Nnat decreased the mRNA levels of Nnat and involucrin, but not of cytokeratin1. Furthermore, a gene transfection assay showed increased involucrin expression in the Nnat-transfected keratinocytes than in mock-transfected counterparts, without any appreciable influence on cytokeratin1, filaggrin, loricrin and S100A7 expression.These data indicate that Nnat is related to keratinocyte differentiation by up-regulating involucrin expression."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380848657196288262","@type":"Researcher","foaf:name":[{"@value":"Long Dugu"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276185289216","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40529848"},{"@type":"NRID","@value":"1000040529848"},{"@type":"NRID","@value":"9000316216597"},{"@type":"NRID","@value":"9000257938484"},{"@type":"NRID","@value":"9000403958761"},{"@type":"NRID","@value":"9000274300700"},{"@type":"NRID","@value":"9000414742173"}],"foaf:name":[{"@value":"Takeshi Nakahara"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288258","@type":"Researcher","foaf:name":[{"@value":"Zhouwei Wu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288261","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Uchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288260","@type":"Researcher","foaf:name":[{"@value":"Min Liu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288263","@type":"Researcher","foaf:name":[{"@value":"Katsuya Hirano"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288256","@type":"Researcher","foaf:name":[{"@value":"Takehiko Yokomizo"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848657196288257","@type":"Researcher","foaf:name":[{"@value":"Masutaka Furue"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09231811"}],"prism:publicationName":[{"@value":"Journal of Dermatological Science"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2014-03","prism:volume":"73","prism:number":"3","prism:startingPage":"225","prism:endingPage":"231"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0923181113003538?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0923181113003538?httpAccept=text/plain"}],"createdAt":"2013-10-30","modifiedAt":"2025-10-13","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Keratinocytes","dc:title":"Keratinocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Membrane%20Proteins","dc:title":"Membrane Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Differentiation","dc:title":"Cell Differentiation"},{"@id":"https://cir.nii.ac.jp/all?q=Nerve%20Tissue%20Proteins","dc:title":"Nerve Tissue Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Filaggrin%20Proteins","dc:title":"Filaggrin Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Up-Regulation","dc:title":"Up-Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Keratins","dc:title":"Keratins"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Precursors","dc:title":"Protein Precursors"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782102325760","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22116002"},{"@type":"JGN","@value":"JP22116002"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-22116002/"}],"notation":[{"@language":"ja","@value":"ロイコトリエン受容体と新規脂溶性リガンドの探索"},{"@language":"en","@value":"Leukotriene receptors and new lipid ligands"}]},{"@id":"https://cir.nii.ac.jp/crid/1040000782274124416","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25860951"},{"@type":"JGN","@value":"JP25860951"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25860951/"}],"notation":[{"@language":"ja","@value":"マウス悪性黒色腫発育早期の腫瘍浸潤樹状細胞の解析　－経時的変化とその意義－"},{"@language":"en","@value":"Phenotypic and functional change of tumor-infiltrating dendritic cells during murine melanoma growth."}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257079058432","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"22116001"},{"@type":"JGN","@value":"JP22116001"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-22116001/"}],"notation":[{"@language":"ja","@value":"「脂質マシナリー」領域研究の推進"},{"@language":"en","@value":"Lipid machinery"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004237443964800","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011145417719680","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Abundant expression of neuronatin in normal eccrine, apocrine and sebaceous glands and their neoplasms"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292618903134592","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin mRNA: alternatively spliced forms of a novel brain-specific mammalian developmental gene"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620163354368","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Segment-Specific Expression of the neuronatin Gene during Early Hindbrain Development"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292621152425856","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Crucial roles of neuronatin in insulin secretion and high glucose-induced apoptosis in pancreatic β-cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165959089536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Recent advances in atopic dermatitis and psoriasis: Genetic background, barrier function, and therapeutic targets"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181199862656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression studies of neuronatin in prenatal and postnatal rat pituitary"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185467130496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The leukotriene B<sub>4</sub> receptor <scp>BLT</scp>2 protects barrier function via actin polymerization with phosphorylation of myosin phosphatase target subunit 1 in human keratinocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574094889960576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Focal Nature of Darier's Disease Lesions: Calcium Pumps, Stress, and Mutation?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095140425088","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin Promotes Neural Lineage in ESCs via Ca2+ Signaling"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095540813056","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cell Density and Culture Factors Regulate Keratinocyte Commitment to Differentiation and Expression of Suprabasal K1/K10 Keratins"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574095609322240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin mRNA in PC12 cells: downregulation by nerve growth factor"}]},{"@id":"https://cir.nii.ac.jp/crid/1360574096578024064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Epidermal differentiation: The role of proteases and their inhibitors"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568589827712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Imprinted Gene <i>Neuronatin</i> Is Regulated by Metabolic Status and Associated With Obesity"}]},{"@id":"https://cir.nii.ac.jp/crid/1361131421343398784","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Dynamic Changes in the Localization of Neuronatin-Positive Cells during Neurogenesis in the Embryonic Rat Brain"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045945404032","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Up-Regulation of Transient Receptor Potential Canonical 1 (TRPC1) following Sarco(endo)plasmic Reticulum Ca<sup>2+</sup>ATPase 2 Gene Silencing Promotes Cell Survival: A Potential Role for TRPC1 in Darier's Disease"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418519308058240","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Molecular Cloning of a Novel mRNA (Neuronatin) That Is Highly Expressed in Neonatal Mammalian Brain"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520928709504","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Prevalence of cardiovascular risk factors in patients with psoriasis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699993917227904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995032003584","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cloning of humanneuronatin gene and its localization to chromosome-20q11.2–12: the deduced protein is a novel ‘proteolipid’"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995120420736","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Calcium regulation of growth and differentiation of mouse epidermal cells in culture"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995419431296","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the ‘fused genes’ family"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419681502208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Functional Characterization of the Dendritically Localized mRNA Neuronatin in Hippocampal Neurons"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368316777472","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Coexpression of neuronatin splice forms promotes medulloblastoma growth"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843427296128","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Psoriasis and metabolic syndrome"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845889327488","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin, a Downstream Target of BETA2/NeuroD1 in the Pancreas, Is Involved in Glucose-Mediated Insulin Secretion"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319422333056","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670321295099904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Peg5/Neuronatin is an imprinted gene located on sub-distal chromosome 2 in the mouse"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794220919936","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"High-Cell-Density Phorbol Ester and Retinoic Acid Upregulate Involucrin and Downregulate Suprabasal Keratin 10 in Autocrine Cultures of Human Epidermal Keratinocytes"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794762328064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neuronatin in a Subset of Glioblastoma Multiforme Tumor Progenitor Cells Is Associated with Increased Cell Proliferation and Shorter Patient Survival"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268777364224","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Lafora disease ubiquitin ligase malin promotes proteasomal degradation of neuronatin and regulates glycogen synthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268793243520","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Specific gene expression in pancreatic beta-cells: cloning and characterization of differentially expressed genes."}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269606542720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Calcium- and vitamin D-regulated keratinocyte differentiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1372539179750692612","@type":"Product","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Aberrant expression of tenascin-c and neuronatin in malignant peripheral nerve sheath tumors"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.jdermsci.2013.10.008"},{"@type":"KAKEN","@value":"PRODUCT-13171488"},{"@type":"KAKEN","@value":"PRODUCT-15166974"},{"@type":"KAKEN","@value":"PRODUCT-13171012"},{"@type":"OPENAIRE","@value":"doi_dedup___::ff6347b963e7d6d572c8d529eddc465f"},{"@type":"CROSSREF","@value":"10.1369/0022155419836433_references_DOI_Z07ipAFTz47GMX8Akh7j65c598k"},{"@type":"CROSSREF","@value":"10.1159/000504359_references_DOI_Z07ipAFTz47GMX8Akh7j65c598k"},{"@type":"CROSSREF","@value":"10.1007/s00441-015-2325-2_references_DOI_Z07ipAFTz47GMX8Akh7j65c598k"},{"@type":"CROSSREF","@value":"10.1111/exd.12976_references_DOI_Z07ipAFTz47GMX8Akh7j65c598k"},{"@type":"CROSSREF","@value":"10.1016/j.jdermsci.2015.02.010_references_DOI_Z07ipAFTz47GMX8Akh7j65c598k"}]}