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Several studies indicate the presence of cells with adipogenic potential in skeletal muscle. However, the mechanism of fate specification that triggers these cells to enter an adipogenic program <jats:italic>in vivo</jats:italic> remains to be solved. In the present study, we examined whether activation of the adipogenic program of muscle‐resident cells precedes their proliferation upon muscle injury. For this purpose, muscle injury was induced by injecting bupivacaine (<jats:styled-content style=\"fixed-case\">BPVC</jats:styled-content>) to excised skeletal muscle <jats:italic>ex vivo</jats:italic>. Cells isolated from <jats:italic>ex vivo</jats:italic> <jats:styled-content style=\"fixed-case\">BPVC</jats:styled-content>‐treated muscle exhibited higher adipogenic potential than those from saline‐treated muscle. Pre‐plating exposure of skeletal muscle cells to basic fibroblast growth factor (<jats:styled-content style=\"fixed-case\">bFGF</jats:styled-content>) mimicked the effect of <jats:italic>ex vivo</jats:italic> <jats:styled-content style=\"fixed-case\">BPVC</jats:styled-content>‐treatment, suggesting that <jats:styled-content style=\"fixed-case\">bFGF</jats:styled-content> released from extracellular matrix in response to muscle injury activates their adipogenic program. Interestingly, the number of myotubes were significantly reduced in the culture from <jats:styled-content style=\"fixed-case\">BPVC</jats:styled-content>‐treated muscle, suggesting that adipocytes negatively regulate myogenesis.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326223639936","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70272440"},{"@type":"NRID","@value":"1000070272440"},{"@type":"NRID","@value":"9000005513891"},{"@type":"NRID","@value":"9000021003544"},{"@type":"NRID","@value":"9000258525097"},{"@type":"NRID","@value":"9000021089721"},{"@type":"NRID","@value":"9000020757398"},{"@type":"NRID","@value":"9000021076166"},{"@type":"NRID","@value":"9000021016669"},{"@type":"NRID","@value":"9000021003505"},{"@type":"NRID","@value":"9000257774920"},{"@type":"NRID","@value":"9000405637754"},{"@type":"NRID","@value":"9000257754903"},{"@type":"NRID","@value":"9000241503312"},{"@type":"NRID","@value":"9000021036582"},{"@type":"NRID","@value":"9000392068647"},{"@type":"NRID","@value":"9000252836927"},{"@type":"NRID","@value":"9000392063974"},{"@type":"NRID","@value":"9000258524760"},{"@type":"NRID","@value":"9000242250954"},{"@type":"NRID","@value":"9000021067217"},{"@type":"NRID","@value":"9000020108501"},{"@type":"NRID","@value":"9000257774903"},{"@type":"NRID","@value":"9000021076154"},{"@type":"NRID","@value":"9000021066562"},{"@type":"NRID","@value":"9000345253810"},{"@type":"NRID","@value":"9000381456397"},{"@type":"NRID","@value":"9000021050091"},{"@type":"NRID","@value":"9000020828453"},{"@type":"NRID","@value":"9000021954169"},{"@type":"NRID","@value":"9000253231391"},{"@type":"NRID","@value":"9000243879351"},{"@type":"NRID","@value":"9000258525280"},{"@type":"NRID","@value":"9000022059474"},{"@type":"NRID","@value":"9000347102956"},{"@type":"NRID","@value":"9000020933971"},{"@type":"NRID","@value":"9000242072543"},{"@type":"NRID","@value":"9000392069612"},{"@type":"NRID","@value":"9000021003874"},{"@type":"NRID","@value":"9000021078314"},{"@type":"NRID","@value":"9000020486454"},{"@type":"NRID","@value":"9000021952498"},{"@type":"NRID","@value":"9000253285821"},{"@type":"NRID","@value":"9000392063432"},{"@type":"NRID","@value":"9000410885292"},{"@type":"NRID","@value":"9000383086157"},{"@type":"NRID","@value":"9000413750522"},{"@type":"NRID","@value":"9000363382472"},{"@type":"NRID","@value":"9000261008767"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0046355"}],"foaf:name":[{"@value":"Keitaro Yamanouchi"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo  Bunkyo Tokyo Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660408201351","@type":"Researcher","foaf:name":[{"@value":"Katsuyuki Nakamura"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo  Bunkyo Tokyo Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660408201219","@type":"Researcher","foaf:name":[{"@value":"Yuki Takegahara"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo  Bunkyo Tokyo Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380579812473781505","@type":"Researcher","foaf:name":[{"@value":"Shin‐ichi Nakano"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo  Bunkyo Tokyo Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660408201352","@type":"Researcher","foaf:name":[{"@value":"Masugi Nishihara"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Physiology Graduate School of Agricultural and Life Sciences The University of Tokyo  Bunkyo Tokyo Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13443941"},{"@type":"EISSN","@value":"17400929"}],"prism:publicationName":[{"@value":"Animal Science 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