{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360848660894815232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aac.02587-16"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AAC.02587-16"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Role of\n            <i>psl</i>\n            Genes in Antibiotic Tolerance of Adherent Pseudomonas aeruginosa"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Bacteria attached to a surface are generally more tolerant to antibiotics than their planktonic counterparts, even without the formation of a biofilm. The mechanism of antibiotic tolerance in biofilm communities is multifactorial, and the genetic background underlying this antibiotic tolerance has not yet been fully elucidated. Using transposon mutagenesis, we isolated a mutant with reduced tolerance to biapenem (relative to that of the wild type) from adherent cells. Sequencing analysis revealed a mutation in the\n            <jats:italic>pslL</jats:italic>\n            gene, which is part of the polysaccharide biosynthesis operon. The\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa</jats:named-content>\n            PAO1Δ\n            <jats:italic>pslBCD</jats:italic>\n            mutant demonstrated a 100-fold-lower survival rate during the exposure of planktonic and biofilm cells to biapenem; a similar phenotype was observed in a mouse infection model and in clinical strains. Transcriptional analysis of adherent cells revealed increased expression of both\n            <jats:italic>pslA</jats:italic>\n            and\n            <jats:italic>pelA</jats:italic>\n            , which are directly regulated by bis-(3′,5′)-cyclic dimeric GMP (c-di-GMP). Inactivation of\n            <jats:italic>wspF</jats:italic>\n            resulted in significantly increased tolerance to biapenem due to increased production of c-di-GMP. The loss of\n            <jats:italic>pslBCD</jats:italic>\n            in the Δ\n            <jats:italic>wspF</jats:italic>\n            mutant background abolished the biapenem-tolerant phenotype of the Δ\n            <jats:italic>wspF</jats:italic>\n            mutant, underscoring the importance of\n            <jats:italic>psl</jats:italic>\n            in biapenem tolerance. Overexpression of PA2133, which can catalyze the degradation of c-di-GMP, led to a significant reduction in biapenem tolerance in adherent cells, indicating that c-di-GMP is essential in mediating the tolerance effect. The effect of\n            <jats:italic>pslBCD</jats:italic>\n            on antibiotic tolerance was evident, with 50- and 200-fold-lower survival in the presence of ofloxacin and tobramycin, respectively. We speculate that the\n            <jats:italic>psl</jats:italic>\n            genes, which are activated by surface adherence through elevated intracellular c-di-GMP levels, confer tolerance to antimicrobials.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380848660894815117","@type":"Researcher","foaf:name":[{"@value":"Keiji Murakami"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894814981","@type":"Researcher","foaf:name":[{"@value":"Tsuneko Ono"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Microbiology, Institute of Health Biosciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894815239","@type":"Researcher","foaf:name":[{"@value":"Darija Viducic"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894815236","@type":"Researcher","foaf:name":[{"@value":"Yoko Somiya"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Microbiology, Institute of Health Biosciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801185743616","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40112148"},{"@type":"NRID","@value":"1000040112148"},{"@type":"NRID","@value":"9000239192100"},{"@type":"NRID","@value":"9000253065939"},{"@type":"NRID","@value":"9000024823145"},{"@type":"NRID","@value":"9000021589939"},{"@type":"NRID","@value":"9000006244547"},{"@type":"NRID","@value":"9000347253460"},{"@type":"NRID","@value":"9000391601214"},{"@type":"NRID","@value":"9000253012230"},{"@type":"NRID","@value":"9000391602744"},{"@type":"NRID","@value":"9000392757728"},{"@type":"NRID","@value":"9000022461575"},{"@type":"NRID","@value":"9000009532059"},{"@type":"NRID","@value":"9000255838470"},{"@type":"NRID","@value":"9000011052033"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0193172"}],"foaf:name":[{"@value":"Reiko Kariyama"}],"jpcoar:affiliationName":[{"@value":"Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Shikata-cho, Okayama, Japan"},{"@value":"Department of Food and Nutrition, Okayama Gakuin University, Aruki, Kurashiki, Okayama, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1030566608870637827","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000347253461"}],"foaf:name":[{"@value":"Kenji Hori"}],"jpcoar:affiliationName":[{"@value":"Innovation Center Okayama for Nanobio-Targeted Therapy, Okayama University, Shikata-cho, Okayama, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894814988","@type":"Researcher","foaf:name":[{"@value":"Takashi Amoh"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894815106","@type":"Researcher","foaf:name":[{"@value":"Katsuhiko Hirota"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894814850","@type":"Researcher","foaf:name":[{"@value":"Hiromi Kumon"}],"jpcoar:affiliationName":[{"@value":"Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Shikata-cho, Okayama, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894814857","@type":"Researcher","foaf:name":[{"@value":"Matthew R. Parsek"}],"jpcoar:affiliationName":[{"@value":"Department of Microbiology, University of Washington, Seattle, Washington, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1380848660894815107","@type":"Researcher","foaf:name":[{"@value":"Yoichiro Miyake"}],"jpcoar:affiliationName":[{"@value":"Department of Oral Microbiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto-cho, Tokushima, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00664804"},{"@type":"EISSN","@value":"10986596"}],"prism:publicationName":[{"@value":"Antimicrobial Agents and Chemotherapy"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2017-07","prism:volume":"61","prism:number":"7","prism:startingPage":"e02587-16"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AAC.02587-16"}],"createdAt":"2017-04-25","modifiedAt":"2022-02-21","project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257236805376","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25461512"},{"@type":"JGN","@value":"JP25461512"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25461512/"}],"notation":[{"@language":"ja","@value":"先進的リアルタイムイメージング法によるクオラムセンシング阻害剤の作用機序の解析"},{"@language":"en","@value":"Analyses of mechanism of action of a quorum sensing inhibitor by using real-time imaging system"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050585658870890624","@type":"Article","resourceType":"学術雑誌論文(journal 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