{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360848664888916352.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.4161/psb.22086"}},{"identifier":{"@type":"URI","@value":"https://www.tandfonline.com/doi/pdf/10.4161/psb.22086"}},{"identifier":{"@type":"PMID","@value":"22990444"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Intracellular localization and physiological function of a rice Ca<sup>2+</sup>-permeable channel OsTPC1"}],"description":[{"notation":[{"@value":"Two-pore channels (TPCs) are cation channels with a voltage-sensor domain conserved in plants and animals. Rice OsTPC1 is predominantly localized to the plasma membrane (PM), and assumed to play an important role as a Ca²⁺-permeable cation channel in the regulation of cytosolic Ca²⁺ rise and innate immune responses including hypersensitive cell death and phytoalexin biosynthesis in cultured rice cells triggered by a fungal elicitor, xylanase from Trichoderma viride. In contrast, Arabidopsis AtTPC1 is localized to the vacuolar membrane (VM). To gain further insights into the intracellular localization of OsTPC1, we stably expressed OsTPC1-GFP in tobacco BY-2 cells. Confocal imaging and membrane fractionation revealed that, unlike in rice cells, the majority of OsTPC1-GFP fusion protein was targeted to the VM in tobacco BY-2 cells. Intracellular localization and functions of the plant TPC family is discussed."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326210615936","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50422499"},{"@type":"NRID","@value":"1000050422499"},{"@type":"NRID","@value":"9000391937305"},{"@type":"NRID","@value":"9000391945023"},{"@type":"NRID","@value":"9000274299959"},{"@type":"NRID","@value":"9000391967008"},{"@type":"NRID","@value":"9000391924572"},{"@type":"NRID","@value":"9000391970211"},{"@type":"NRID","@value":"9000391552005"},{"@type":"NRID","@value":"9000000943075"},{"@type":"NRID","@value":"9000273020540"},{"@type":"NRID","@value":"9000391943404"},{"@type":"NRID","@value":"9000345431575"},{"@type":"NRID","@value":"9000018660961"},{"@type":"NRID","@value":"9000391950772"},{"@type":"NRID","@value":"9000391948147"},{"@type":"NRID","@value":"9000391935689"},{"@type":"NRID","@value":"9000403160909"},{"@type":"NRID","@value":"9000275823160"},{"@type":"NRID","@value":"9000391920774"},{"@type":"NRID","@value":"9000391926520"},{"@type":"NRID","@value":"9000391958942"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0158278"}],"foaf:name":[{"@value":"Takamitsu 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Kuchitsu"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Biological Science; Tokyo University of Science; 2641 Yamazaki, Noda, Chiba, Japan"},{"@value":"Research Institute for Science and Technology; Tokyo University of Science; 2641 Yamazaki, Noda, Chiba, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15592324"}],"prism:publicationName":[{"@value":"Plant Signaling & Behavior"}],"dc:publisher":[{"@value":"Informa UK Limited"}],"prism:publicationDate":"2012-09-18","prism:volume":"7","prism:number":"11","prism:startingPage":"1428","prism:endingPage":"1430"},"reviewed":"false","url":[{"@id":"https://www.tandfonline.com/doi/pdf/10.4161/psb.22086"}],"createdAt":"2012-10-26","modifiedAt":"2022-08-31","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Fungal%20Proteins","dc:title":"Fungal 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cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679304731904","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice"},{"@value":"Evidence for the plasma membrane localization of a putative voltage-dependent Ca〔2+〕 channel, OsTPC1, in rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679306115328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Regulation of xylanase elicitor-induced expression of defense-related genes involved in phytoalexin biosynthesis by a cation channel OsTPC1 in suspension-cultured rice cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1520291855407557760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Dynamic Organization of Vacuolar and Microtubule Structures during Cell Cycle 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