{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360853567852492416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.reprotox.2021.03.008"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0890623821000587?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0890623821000587?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"33845160"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Neonatal maternal separation increases the number of p27-positive Sertoli cells in prepuberty"}],"description":[{"notation":[{"@value":"Neonatal maternal separation (NMS) is an experimental model for early life stress, which affects the growth and development of various organs, resulting in adverse health effects in humans and animals. In our previous study, we demonstrated that NMS [(0.5-, 1-, 2-h/day NMS, from postnatal day (PND) 1-10] induced morphological changes to the male reproductive system, including decreased Sertoli cell numbers in mouse testes at PND 70. To clarify the mechanism by which NMS decreases Sertoli cell numbers, we evaluated the effects of NMS on mouse testes at PNDs 10 and 16. At PND 10, the Sertoli cell number was not significantly different among experimental groups; however, it decreased in 0.5- and 2-h/day NMS mice at PND 16. The termination of Sertoli cell proliferation in prepuberty can be induced by p27, a cyclin-dependent kinase inhibitor. At PND 10, we observed an increase in the number of p27-positive Sertoli cells in 2-h/day NMS mice. The seminiferous tubule diameters decreased significantly in 1- and 2-h/day NMS mice, and the relative interstitial area increased in 2-h/day NMS mice. Serum corticosterone level significantly increased, and serum testosterone level significantly decreased in the 2-h/day NMS mice. At PND 16, the tubule diameters and height of seminiferous epithelium were significantly higher in 0.5- and 2-h/day NMS mice. Our results suggest that NMS disturbs serum corticosterone and testosterone levels and increases the number of p27-positive Sertoli cells at PND 10, resulting in a decrease in the number of Sertoli cells at PND 16."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801201769216","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80422252"},{"@type":"NRID","@value":"1000080422252"},{"@type":"NRID","@value":"9000257820810"},{"@type":"NRID","@value":"9000257823233"},{"@type":"NRID","@value":"9000345282378"},{"@type":"NRID","@value":"9000240035866"},{"@type":"NRID","@value":"9000001871750"},{"@type":"NRID","@value":"9000413736344"},{"@type":"NRID","@value":"9000006175076"},{"@type":"NRID","@value":"9000412152283"},{"@type":"NRID","@value":"9000411248463"},{"@type":"NRID","@value":"9000413930976"},{"@type":"NRID","@value":"9000258730805"},{"@type":"NRID","@value":"9000411586142"},{"@type":"NRID","@value":"9000412653709"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/18202532-zg"}],"foaf:name":[{"@value":"Hidenobu Miyaso"}]},{"@id":"https://cir.nii.ac.jp/crid/1380853567852492676","@type":"Researcher","foaf:name":[{"@value":"Kaiya 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Toxicology"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2021-06","prism:volume":"102","prism:startingPage":"56","prism:endingPage":"66"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license","https://doi.org/10.15223/policy-017","https://doi.org/10.15223/policy-037","https://doi.org/10.15223/policy-012","https://doi.org/10.15223/policy-029","https://doi.org/10.15223/policy-004"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0890623821000587?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0890623821000587?httpAccept=text/plain"}],"createdAt":"2021-04-16","modifiedAt":"2025-10-11","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Sertoli%20Cells","dc:title":"Sertoli Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Estradiol","dc:title":"Estradiol"},{"@id":"https://cir.nii.ac.jp/all?q=Maternal%20Deprivation","dc:title":"Maternal Deprivation"},{"@id":"https://cir.nii.ac.jp/all?q=Seminiferous%20Tubules","dc:title":"Seminiferous Tubules"},{"@id":"https://cir.nii.ac.jp/all?q=Spermatozoa","dc:title":"Spermatozoa"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Testis","dc:title":"Testis"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Testosterone","dc:title":"Testosterone"},{"@id":"https://cir.nii.ac.jp/all?q=Follicle%20Stimulating%20Hormone","dc:title":"Follicle Stimulating Hormone"},{"@id":"https://cir.nii.ac.jp/all?q=Spermatogenesis","dc:title":"Spermatogenesis"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Proliferation","dc:title":"Cell 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