{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360855569943334656.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1365-313x.2007.03157.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-313X.2007.03157.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-313X.2007.03157.x"}}],"dc:title":[{"@value":"Transgenic, non‐isoprene emitting poplars don’t like it hot"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>The physiological role of isoprene emission in plants is a matter of much debate. One of the most widely propagated hypotheses suggests a function of isoprene in the protection of leaf physiological processes against thermal and oxidative stress. To test this hypothesis, we developed transgenic Grey poplar (<jats:italic>Populus</jats:italic>×<jats:italic>canescens</jats:italic>) plants in which gene expression of isoprene synthase (ISPS) was either silenced by RNA interference (RNAi) or upregulated by over‐expression of the <jats:italic>ISPS</jats:italic> gene. Despite increased <jats:italic>ISPS</jats:italic> mRNA levels, we did not observe consistent increases in isoprene emission in the over‐expressing lines, indicating post‐transcriptional control of <jats:italic>ISPS</jats:italic> by co‐suppression. In the RNAi lines, levels of isoprene emission were effectively suppressed to virtually zero. Transgenic plants were subjected to temperature stress with three transient heat phases of 38–40°C, each followed by phases of recovery at 30°C. Parallel measurements of gas exchange, chlorophyll fluorescence and isoprene emission provided new insights into the physiological link between isoprene and enhanced temperature tolerance. Transgenic non‐isoprene‐emitting poplars showed reduced rates of net assimilation and photosynthetic electron transport during heat stress, but not in the absence of stress. The decrease in the efficiency of photochemistry was inversely correlated with the increase in heat dissipation of absorbed light energy, measured as NPQ (non‐photochemical quenching). Isoprene‐repressed poplars also displayed an increased formation of the xanthophyll cycle pigment zeaxanthin in the absence of stress, which can cause increased NPQ or may indicate an increased requirement for antioxidants. In conclusion, using a molecular genetic approach, we show that down‐regulation of isoprene emission affects thermotolerance of photosynthesis and induces increased energy dissipation by NPQ pathways.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380855569943334664","@type":"Researcher","foaf:name":[{"@value":"Katja Behnke"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334662","@type":"Researcher","foaf:name":[{"@value":"Barbara Ehlting"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334658","@type":"Researcher","foaf:name":[{"@value":"Markus Teuber"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334656","@type":"Researcher","foaf:name":[{"@value":"Martina Bauerfeind"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334663","@type":"Researcher","foaf:name":[{"@value":"Sandrine Louis"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334661","@type":"Researcher","foaf:name":[{"@value":"Robert Hänsch"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334659","@type":"Researcher","foaf:name":[{"@value":"Andrea Polle"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855569943334657","@type":"Researcher","foaf:name":[{"@value":"Jörg Bohlmann"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016867742845184","@type":"Researcher","foaf:name":[{"@value":"Jörg‐Peter Schnitzler"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09607412"},{"@type":"EISSN","@value":"1365313X"}],"prism:publicationName":[{"@value":"The Plant Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-06-21","prism:volume":"51","prism:number":"3","prism:startingPage":"485","prism:endingPage":"499"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-313X.2007.03157.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-313X.2007.03157.x"}],"createdAt":"2007-06-22","modifiedAt":"2023-10-10","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050011848869271168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Isoprene emission characteristics of tall and dwarf bamboos"}]},{"@id":"https://cir.nii.ac.jp/crid/1360865815486470912","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Quantifying variabilities in monoterpene emission among natural individuals of an urban-greening species (Quercus phylliraeoides) using a rapid measuring method"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303560704","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The genetic basis of foliar terpene yield: Implications for breeding and profitability of Australian essential oil crops"}]},{"@id":"https://cir.nii.ac.jp/crid/1391131406304660096","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Review: Exchanges of volatile organic compounds between terrestrial ecosystems and the atmosphere"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1365-313x.2007.03157.x"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.1009a_references_DOI_Nu47pZ6A8PjYdg6siHxAz9L3Yca"},{"@type":"CROSSREF","@value":"10.1016/j.aeaoa.2021.100136_references_DOI_Nu47pZ6A8PjYdg6siHxAz9L3Yca"},{"@type":"CROSSREF","@value":"10.2480/agrmet.d-20-00025_references_DOI_Nu47pZ6A8PjYdg6siHxAz9L3Yca"},{"@type":"CROSSREF","@value":"10.1016/j.ufug.2024.128245_references_DOI_Nu47pZ6A8PjYdg6siHxAz9L3Yca"}]}