{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360855570555870976.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1371/journal.pone.0015650"}},{"identifier":{"@type":"URI","@value":"http://dx.plos.org/10.1371/journal.pone.0015650"}},{"identifier":{"@type":"PMID","@value":"21187926"}}],"dc:title":[{"@value":"Macroautophagy-Mediated Degradation of Whole Nuclei in the Filamentous Fungus Aspergillus oryzae"}],"description":[{"notation":[{"@value":"Filamentous fungi consist of continuum of multinucleate cells called hyphae, and proliferate by means of hyphal tip growth. Accordingly, research interest has been focusing on hyphal tip cells, but little is known about basal cells in colony interior that do not directly contribute to proliferation. Here, we show that autophagy mediates degradation of basal cell components in the filamentous fungus Aspergillus oryzae. In basal cells, enhanced green fluorescent protein (EGFP)-labeled peroxisomes, mitochondria, and even nuclei were taken up into vacuoles in an autophagy-dependent manner. During this process, crescents of autophagosome precursors matured into ring-like autophagosomes to encircle apparently whole nuclei. The ring-like autophagosomes then disappeared, followed by dispersal of the nuclear material throughout the vacuoles, suggesting the autophagy-mediated degradation of whole nuclei. We also demonstrated that colony growth in a nutrient-depleted medium was significantly inhibited in the absence of functional autophagy. This is a first report describing autophagy-mediated degradation of whole nuclei, as well as suggesting a novel strategy of filamentous fungi to degrade components of existing hyphae for use as nutrients to support mycelial growth in order to counteract starvation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380855570555870977","@type":"Researcher","foaf:name":[{"@value":"Jun-ya Shoji"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204146286339","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000001862447"},{"@type":"NRID","@value":"9000391396794"},{"@type":"NRID","@value":"9000323308114"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0204459"}],"foaf:name":[{"@value":"Takashi Kikuma"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855570555870978","@type":"Researcher","foaf:name":[{"@value":"Manabu Arioka"}]},{"@id":"https://cir.nii.ac.jp/crid/1380855570555870980","@type":"Researcher","foaf:name":[{"@value":"Katsuhiko Kitamoto"}]}],"contributor":[{"@id":"https://cir.nii.ac.jp/crid/1380855570555870976","@type":"Researcher","foaf:name":[{"@value":"Diane Bassham"}],"role":"editor"}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"19326203"}],"prism:publicationName":[{"@value":"PLoS ONE"}],"dc:publisher":[{"@value":"Public Library of Science (PLoS)"}],"prism:publicationDate":"2010-12-20","prism:volume":"5","prism:number":"12","prism:startingPage":"e15650"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"http://dx.plos.org/10.1371/journal.pone.0015650"}],"createdAt":"2010-12-20","modifiedAt":"2020-05-09","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Nucleus","dc:title":"Cell Nucleus"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=Aspergillus%20oryzae","dc:title":"Aspergillus oryzae"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=Green%20Fluorescent%20Proteins","dc:title":"Green Fluorescent Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=R","dc:title":"R"},{"@id":"https://cir.nii.ac.jp/all?q=Polymerase%20Chain%20Reaction","dc:title":"Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Mitochondria","dc:title":"Mitochondria"},{"@id":"https://cir.nii.ac.jp/all?q=Open%20Reading%20Frames","dc:title":"Open Reading Frames"},{"@id":"https://cir.nii.ac.jp/all?q=Phagosomes","dc:title":"Phagosomes"},{"@id":"https://cir.nii.ac.jp/all?q=Vacuoles","dc:title":"Vacuoles"},{"@id":"https://cir.nii.ac.jp/all?q=Autophagy","dc:title":"Autophagy"},{"@id":"https://cir.nii.ac.jp/all?q=Peroxisomes","dc:title":"Peroxisomes"},{"@id":"https://cir.nii.ac.jp/all?q=Medicine","dc:title":"Medicine"},{"@id":"https://cir.nii.ac.jp/all?q=Lysosomes","dc:title":"Lysosomes"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Deletion","dc:title":"Gene Deletion"},{"@id":"https://cir.nii.ac.jp/all?q=Research%20Article","dc:title":"Research Article"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232163314432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional analysis of AoAtg11 in selective autophagy in the filamentous fungus Aspergillus 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