{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360857593669969792.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41598-021-90020-0"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-021-90020-0.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41598-021-90020-0"}},{"identifier":{"@type":"PMID","@value":"34006987"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>G protein-coupled receptor kinase 5 (GRK5) regulates inflammatory responses via the nuclear factor-kappa B (NF-κB) pathway. This study investigated the functional involvement of GRK5 in the pathogenesis of inflammatory arthritis. Immunohistochemically, rheumatoid arthritis (RA) synovium had a significantly higher proportion of GRK5-positive cells in the synovial lining layer than healthy control synovium. Gene expression and NF-κB activation in lipopolysaccharide-stimulated human SW982 synovial cells were significantly suppressed by silencing of the <jats:italic>GRK5</jats:italic> gene. Similarly, GRK5 kinase activity inhibition in human primary RA synovial cells attenuated gene expressions of inflammatory factors. In a murine model of collagen antibody-induced arthritis, arthritis scores and serum IL6 production of <jats:italic>GRK5</jats:italic> knockout (GRK5<jats:sup>-/-</jats:sup>) mice were significantly lower than those of wild-type mice. Histologically, the degree of synovitis and cartilage degeneration in GRK5<jats:sup>-/-</jats:sup> mice was significantly lower than in wild-type mice. In in vitro analyses using activated murine macrophages and fibroblast-like synoviocytes, gene expression of inflammatory factors and p65 nuclear translocation were significantly lower in GRK5<jats:sup>-/-</jats:sup> mice compared to wild-type mice. In conclusion, our results suggested that GRK5 is deeply involved in the pathogenesis of inflammatory arthritis, therefore, GRK5 inhibition could be a potential therapeutic target for types of inflammatory arthritis such as RA.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380857593669969818","@type":"Researcher","foaf:name":[{"@value":"Masakazu Toya"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751142021888","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60568963"},{"@type":"NRID","@value":"1000060568963"},{"@type":"NRID","@value":"9000345402549"},{"@type":"NRID","@value":"9000016805681"},{"@type":"NRID","@value":"9000403293023"}],"foaf:name":[{"@value":"Yukio Akasaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969820","@type":"Researcher","foaf:name":[{"@value":"Takuya Sueishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969817","@type":"Researcher","foaf:name":[{"@value":"Ichiro Kurakazu"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969927","@type":"Researcher","foaf:name":[{"@value":"Masanari Kuwahara"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969937","@type":"Researcher","foaf:name":[{"@value":"Taisuke Uchida"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969819","@type":"Researcher","foaf:name":[{"@value":"Tomoaki Tsutsui"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969813","@type":"Researcher","foaf:name":[{"@value":"Hidetoshi Tsushima"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969797","@type":"Researcher","foaf:name":[{"@value":"Hisakata Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969670","@type":"Researcher","foaf:name":[{"@value":"Martin K. Lotz"}]},{"@id":"https://cir.nii.ac.jp/crid/1380857593669969671","@type":"Researcher","foaf:name":[{"@value":"Yasuharu Nakashima"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20452322"}],"prism:publicationName":[{"@value":"Scientific Reports"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2021-05-18","prism:volume":"11","prism:number":"1"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41598-021-90020-0.pdf"},{"@id":"https://www.nature.com/articles/s41598-021-90020-0"}],"createdAt":"2021-05-18","modifiedAt":"2022-12-03","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=G-Protein-Coupled%20Receptor%20Kinase%205","dc:title":"G-Protein-Coupled Receptor Kinase 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