Phospholipase D activation is required for 1-aminocyclopropane 1-carboxylic acid signaling during sexual reproduction in the marine red alga Neopyropia yezoensis (Rhodophyta)

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Bibliographic Information

Published
2022-04-08
Resource Type
journal article
Rights Information
  • https://creativecommons.org/licenses/by/4.0
  • https://creativecommons.org/licenses/by/4.0
DOI
  • 10.1186/s12870-022-03575-z
Publisher
Springer Science and Business Media LLC

Description

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>1-aminocyclopropane 1-carboxylic acid (ACC) is the immediate precursor of the plant hormone ethylene. However, recent studies have suggested that ACC also acts as a signaling molecule to regulate development and growth independently from ethylene biosynthesis. In red algae, ACC stimulates the switch from a vegetative to a sexual reproductive phase. However, despite evidence that ACC signaling in plants and algae is widespread, the mechanistic basis of the ACC signaling pathway remains unknown.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>We demonstrate that exogenous ACC increased the activity of phospholipase D (PLD) and induced the accumulation of PLD transcripts in the marine red alga <jats:italic>Neopyropia yezoensis</jats:italic>. The product of PLD, the lipid second messenger phosphatidic acid (PA), also increased in response to ACC. Furthermore, the pharmacological inhibition of PLD by 1-butanol blocked ACC-induced spermatangia and carpospore production, but the inactive isomer t-butanol did not. In addition, 1-butanol prevented ACC-induced growth inhibition and inhibited transcript accumulation of genes upregulated by ACC, including extracellular matrix (ECM)-related genes, and alleviated the transcriptional decrease of genes downregulated by ACC, including photosynthesis-related genes.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>These results indicate that PLD is a positive regulator of sexual cell differentiation and a negative regulator of growth. This study demonstrates that PLD and its product, PA, are components of ACC signaling during sexual reproduction in <jats:italic>N. yezoensis</jats:italic>.</jats:p> </jats:sec>

Journal

  • BMC Plant Biology

    BMC Plant Biology 22 (1), 181-, 2022-04-08

    Springer Science and Business Media LLC

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