{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360861290744927232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.1718806115"}},{"identifier":{"@type":"URI","@value":"http://www.pnas.org/syndication/doi/10.1073/pnas.1718806115"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.1718806115"}}],"dc:title":[{"@value":"Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Significance</jats:title>\n          <jats:p>Emerging coronaviruses (CoVs; severe acute respiratory syndrome-CoV and Middle East respiratory syndrome-CoV) pose serious health threats globally, with no specific antiviral treatments available. These viruses are able to faithfully synthesize their large genomic RNA. We report, however, that their main RNA polymerase, nsp12, is not accurate. To achieve accuracy, CoVs have acquired nsp14, a bifunctional enzyme able to methylate the viral RNA cap [methyltransferase (MTase)] and excise erroneous mutagenic nucleotides inserted by nsp12. Strikingly, ribavirin can be excised from the viral genome, thus showing no antiviral activity. The crystal structure of nsp14 shows that it is unique, having been replaced by other MTase types during evolution. This unprecedented RNA correction machinery has allowed RNA genome size expansion, but also provided potential nucleoside drug resistance to these deadly pathogens.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380861290744927234","@type":"Researcher","foaf:name":[{"@value":"François Ferron"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927242","@type":"Researcher","foaf:name":[{"@value":"Lorenzo Subissi"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927233","@type":"Researcher","foaf:name":[{"@value":"Ana Theresa Silveira De Morais"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927241","@type":"Researcher","foaf:name":[{"@value":"Nhung Thi Tuyet Le"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927236","@type":"Researcher","foaf:name":[{"@value":"Marion Sevajol"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927235","@type":"Researcher","foaf:name":[{"@value":"Laure Gluais"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927237","@type":"Researcher","foaf:name":[{"@value":"Etienne Decroly"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927238","@type":"Researcher","foaf:name":[{"@value":"Clemens Vonrhein"}],"jpcoar:affiliationName":[{"@value":"Global Phasing Ltd., Cambridge CB3 0AX, England"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927240","@type":"Researcher","foaf:name":[{"@value":"Gérard Bricogne"}],"jpcoar:affiliationName":[{"@value":"Global Phasing Ltd., Cambridge CB3 0AX, England"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927232","@type":"Researcher","foaf:name":[{"@value":"Bruno Canard"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861290744927239","@type":"Researcher","foaf:name":[{"@value":"Isabelle Imbert"}],"jpcoar:affiliationName":[{"@value":"Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France;"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2017-12-26","prism:volume":"115","prism:number":"2","prism:startingPage":"E162"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"http://www.pnas.org/syndication/doi/10.1073/pnas.1718806115"},{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.1718806115"}],"createdAt":"2017-12-26","modifiedAt":"2022-06-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360584340712938496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex"}]},{"@id":"https://cir.nii.ac.jp/crid/1360588381077703168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Reactive oxygen species-mediated cytotoxic and DNA-damaging mechanism of\n                    <i>N</i>\n                    <sup>4</sup>\n                    -hydroxycytidine, a metabolite of the COVID-19 therapeutic drug molnupiravir"}]},{"@id":"https://cir.nii.ac.jp/crid/2050025942155225216","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular mechanisms of the medicines for COVID-19"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.1718806115"},{"@type":"CROSSREF","@value":"10.1246/bcsj.20220179_references_DOI_2nF0aikP9sd9k37ifyLOavn8Kqv"},{"@type":"CROSSREF","@value":"10.1093/nar/gkad483_references_DOI_2nF0aikP9sd9k37ifyLOavn8Kqv"},{"@type":"CROSSREF","@value":"10.1080/10715762.2025.2469738_references_DOI_2nF0aikP9sd9k37ifyLOavn8Kqv"}]}