{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1360861704787610752.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.3892/mmr.2022.12906"}},{"identifier":{"@type":"PMID","@value":"36484359"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@value":"Function of mitochondrial cytochrome c oxidase is enhanced in human lens epithelial cells at high temperatures"}],"description":[{"notation":[{"@value":"Enhancement of density via human lens epithelium (HLE) cell proliferation is the underlying cause of nuclear cataracts. Moreover, our previous epidemiological study demonstrated that the risk of nuclear cataract development is significantly higher under elevated environmental temperatures compared with under lower temperatures. The present study investigated the relationship between temperature and cell proliferation in terms of mitochondrial function, which is a nuclear cataract‑inducing risk factor, using two different HLE cell lines, SRA01/04 and immortalized human lens epithelial cells NY2 (iHLEC‑NY2). Cell proliferation was significantly enhanced under the high‑temperature condition (37.5˚C) in both cell lines. The cell growth levels of SRA01/04 and iHLEC‑NY2 cells cultured at 37.5˚C were 1.20‑ and 1.16‑fold those in the low‑temperature cultures (35.0˚C), respectively. Moreover, the levels of cytochrome c oxidase mRNA (mitochondrial genome, cytochrome c oxidase‑1‑3) and its activity in SRA01/04 and iHLEC‑NY2 cells cultured at 37.5˚C were higher compared with those in cells cultured at 35.0˚C. In addition, adenosine‑5'‑triphosphate (ATP) levels in SRA01/04 and iHLEC‑NY2 cells were also significantly higher at 37.5˚C compared with those at 35.0˚C. By contrast, no significant differences in Na"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380861704787610881","@type":"Researcher","foaf:name":[{"@value":"Shun Takeda"}],"jpcoar:affiliationName":[{"@value":"Division for Vision Research, Kanazawa Medical University Graduate School of Medical Science, Kahoku, Ishikawa 920‑0293, 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Hatsusaka"}],"jpcoar:affiliationName":[{"@value":"Department of Ophthalmology, Fujita Health University, Toyoake, Aichi 470‑1192, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861704787611011","@type":"Researcher","foaf:name":[{"@value":"Eri Kubo"}],"jpcoar:affiliationName":[{"@value":"Department of Ophthalmology, Fujita Health University, Toyoake, Aichi 470‑1192, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861704787610752","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Sasaki"}],"jpcoar:affiliationName":[{"@value":"Department of Ophthalmology, Fujita Health University, Toyoake, Aichi 470‑1192, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"17912997"},{"@type":"EISSN","@value":"17913004"}],"prism:publicationName":[{"@value":"Molecular Medicine Reports"}],"dc:publisher":[{"@value":"Spandidos 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